LOCUS       sequence-101805-        1085 bp    DNA     linear   UNA 15-DEC-2025
DEFINITION  Expresses PTDH mutant Opt12x.
ACCESSION   .
VERSION     .
KEYWORDS    .
SOURCE      natural DNA sequence
  ORGANISM  unspecified
REFERENCE   1  (bases 1 to 1085)
  AUTHORS   Johannes TW, Woodyer RD, Zhao H
  TITLE     Efficient regeneration of NADPH using an engineered phosphite 
            dehydrogenase.
  JOURNAL   Biotechnol Bioeng. 2007 Jan 1;96(1):18-26.
  PUBMED    16948172
REFERENCE   2  (bases 1 to 1085)
  AUTHORS   .
  TITLE     Direct Submission
  JOURNAL   Exported Dec 15, 2025 from SnapGene Server 8.0.1
            https://www.snapgene.com
COMMENT     SGRef: number: 1; type: "Journal Article"; journalName: "Biotechnol 
            Bioeng."; date: "2007-01-1"; volume: "96(1)"; pages: "18-26"
COMMENT     Sequence Label: pET15b-12x
FEATURES             Location/Qualifiers
     source          1..1085
                     /mol_type="genomic DNA"
                     /organism="unspecified"
     CDS             complement(1040..1057)
                     /codon_start=1
                     /product="thrombin recognition and cleavage site"
                     /label=thrombin site
                     /translation="LVPRGS"
     CDS             complement(1067..1084)
                     /codon_start=1
                     /product="6xHis affinity tag"
                     /label=6xHis
                     /translation="HHHHHH"
ORIGIN
        1 ggctttgtta gcagccggat ccttatcagt ctgcggcagg attggccttg ggcagacggt
       61 tcacagcgtt gattgggcgc tcacctgcca atgcctggag gatgttctgc gctgcacaac
      121 gttcaatctc caggcgcacc gcgcgcactg ccgaccctat gtgcggagtg aacagcgtat
      181 tcggatgcgc gagcagcgca ggatcgatct gctgcggccg gtccgcgcga gcccagtctt
      241 ccatttcgaa tacatccgcc gcatacccgc cgagctggcc tcgctcaagc gccgcgagca
      301 cggcggcttc atccactacc gagccacgac aggggtttac aagcagagcg cccggccgta
      361 cgagggcaag cagctcggcg ttgaccagat gcagggtatc ggcattcaag ggaagcgcca
      421 gcaggatgaa gtccgagctg gcgaagagtt cgctgcacgc cacctggcgc aggccgagcc
      481 gttgctcggt ttgtgtatcc agagccttcg ccgcgtggta ctgcagggtc gcgccccatc
      541 cctgcaagcg atcagccatg gccagtccga tggcgcccat gccaaggaag ccgaccgtag
      601 cgttatccag ccccgtgccg tagaaccgtg gttgccagcc ccggaacttg ccagagcgga
      661 cgaacgcatc tgctgcccgc agatgccgcc ccagccccac cgccagtccg atcgccagct
      721 cggcagtcgg gaccgtcaac agatcaggca cgaaggtcag ccagaccccg cgggcagtac
      781 aggcgtccac atcgaaattg tcgaagccct tgagcgcgca gccgattaca cgcagctcag
      841 ggcaggcttg aagaaagtct gcatcgaccc gatcgggcat gaacgccatc atcgcctgag
      901 catcgcgaca gcggcgcaga atttcctcgc gcgtcagcgt gctgtcggtc tggttggtta
      961 tcagctcgca atgtggcgcc agcagttgca ggatctcttc gtgtactcgg tgagttataa
     1021 cgagtttcgg cagcatatgg ctgccgcgcg gcaccaggcc gctgctgtga tgatgatgat
     1081 gatgg
//
