LOCUS       sequence-101808-         226 bp    DNA     linear   UNA 15-DEC-2025
DEFINITION  encodes sgRNA sequence for targeting mouse Mettl14 locus 
            (Cas9-Nickase strategy).
ACCESSION   .
VERSION     .
KEYWORDS    .
SOURCE      natural DNA sequence
  ORGANISM  unspecified
REFERENCE   1  (bases 1 to 226)
  AUTHORS   Geula S, Moshitch-Moshkovitz S, Dominissini D, Mansour AA, Kol N, 
            Salmon-Divon M, Hershkovitz V, Peer E, Mor N, Manor YS, Ben-Haim MS,
            Eyal E, Yunger S, Pinto Y, Jaitin DA, Viukov S, Rais Y, Krupalnik V,
            Chomsky E, Zerbib M, Maza I, Rechavi Y, Massarwa R, Hanna S, Amit I,
            Levanon EY, Amariglio N, Stern-Ginossar N, Novershtern N, Rechavi G,
            Hanna JH
  TITLE     Stem cells. m6A mRNA methylation facilitates resolution of naive 
            pluripotency toward differentiation.
  JOURNAL   Science. 2015 Feb 27;347(6225):1002-6. doi: 10.1126/science.1261417.
            Epub 2015 Jan 1.
  PUBMED    25569111
REFERENCE   2  (bases 1 to 226)
  AUTHORS   .
  TITLE     Direct Submission
  JOURNAL   Exported Dec 15, 2025 from SnapGene Server 8.0.1
            https://www.snapgene.com
COMMENT     SGRef: number: 1; type: "Journal Article"; doi: 
            "10.1126/science.1261417"; journalName: "Science"; date: 
            "2015-02-27- 27"; volume: "347"; issue: "6225"; pages: "1002-6"
COMMENT     Sequence Label: px335 Mettl14 sgRNA #2
FEATURES             Location/Qualifiers
     source          1..226
                     /mol_type="genomic DNA"
                     /organism="unspecified"
     primer_bind     109..128
                     /label=LKO.1 5'
                     /note="Human U6 promoter, forward primer"
ORIGIN
        1 attggaatta atttgactgt aaacacaaag atattagtac aaaatacgtg acgtacaaag
       61 taataatttc ttgggtagtt tgcagtttta aaattatgtt ttaaaatgga ctatcatatg
      121 cttaccgtaa cttgaaagta tttcgatttc tgtggcttta tatatcttgt ggaaaggacg
      181 aaacaccggc cgctcccgga tctcctgcgt tttagagcta gaaata
//
