LOCUS       sequence-101809-         717 bp    DNA     linear   UNA 15-DEC-2025
DEFINITION  encodes sgRNA sequence for targeting mouse Mettl14 locus 
            (Cas9-Nickase strategy).
ACCESSION   .
VERSION     .
KEYWORDS    .
SOURCE      natural DNA sequence
  ORGANISM  unspecified
REFERENCE   1  (bases 1 to 717)
  AUTHORS   Geula S, Moshitch-Moshkovitz S, Dominissini D, Mansour AA, Kol N, 
            Salmon-Divon M, Hershkovitz V, Peer E, Mor N, Manor YS, Ben-Haim MS,
            Eyal E, Yunger S, Pinto Y, Jaitin DA, Viukov S, Rais Y, Krupalnik V,
            Chomsky E, Zerbib M, Maza I, Rechavi Y, Massarwa R, Hanna S, Amit I,
            Levanon EY, Amariglio N, Stern-Ginossar N, Novershtern N, Rechavi G,
            Hanna JH
  TITLE     Stem cells. m6A mRNA methylation facilitates resolution of naive 
            pluripotency toward differentiation.
  JOURNAL   Science. 2015 Feb 27;347(6225):1002-6. doi: 10.1126/science.1261417.
            Epub 2015 Jan 1.
  PUBMED    25569111
REFERENCE   2  (bases 1 to 717)
  AUTHORS   .
  TITLE     Direct Submission
  JOURNAL   Exported Dec 15, 2025 from SnapGene Server 8.0.1
            https://www.snapgene.com
COMMENT     SGRef: number: 1; type: "Journal Article"; doi: 
            "10.1126/science.1261417"; journalName: "Science"; date: 
            "2015-02-27- 27"; volume: "347"; issue: "6225"; pages: "1002-6"
COMMENT     Sequence Label: px335 Mettl14 sgRNA #2
FEATURES             Location/Qualifiers
     source          1..717
                     /mol_type="genomic DNA"
                     /organism="unspecified"
     CDS             45..71
                     /codon_start=1
                     /product="HA (human influenza hemagglutinin) epitope tag"
                     /label=HA
                     /translation="YPYDVPDYA"
     CDS             75..95
                     /codon_start=1
                     /product="nuclear localization signal of SV40 (simian virus
                     40) large T antigen"
                     /label=SV40 NLS
                     /translation="PKKKRKV"
ORIGIN
        1 cctgcctgaa tcactttttt tcaggttgga ccggtgccac catgtaccca tacgatgttc
       61 cagattacgc ttcgccgaag aaaaagcgca aggtcgaagc gtccgacaag aagtacagca
      121 tcggcctggc catcggcacc aactctgtgg gctgggccgt gatcaccgac gagtacaagg
      181 tgcccagcaa gaaattcaag gtgctgggca acaccgaccg gcacagcatc aagaagaacc
      241 tgatcggagc cctgctgttc gacagcggcg aaacagccga ggccacccgg ctgaagagaa
      301 ccgccagaag aagatacacc agacggaaga accggatctg ctatctgcaa gagatcttca
      361 gcaacgagat ggccaaggtg gacgacagct tcttccacag actggaagag tccttcctgg
      421 tggaagagga taagaagcac gagcggcacc ccatcttcgg caacatcgtg gacgaggtgg
      481 cctaccacga gaagtacccc accatctacc acctgagaaa gaaactggtg gacagcaccg
      541 acaaggccga cctgcggctg atctatctgg ccctggccca catgatcaag ttccggggcc
      601 acttcctgat cgagggcgac ctgaaccccg acaacagcga cgtggacaag ctgttcatcc
      661 agctggtgca gacctacaac cagctgttcg aggaaaaccc catcaacgcc agcggcg
//
