LOCUS       sequence-101811-         799 bp    DNA     linear   UNA 15-DEC-2025
DEFINITION  encodes sgRNA sequence for targeting mouse Mettl14 locus 
            (Cas9-Nickase strategy).
ACCESSION   .
VERSION     .
KEYWORDS    .
SOURCE      natural DNA sequence
  ORGANISM  unspecified
REFERENCE   1  (bases 1 to 799)
  AUTHORS   Geula S, Moshitch-Moshkovitz S, Dominissini D, Mansour AA, Kol N, 
            Salmon-Divon M, Hershkovitz V, Peer E, Mor N, Manor YS, Ben-Haim MS,
            Eyal E, Yunger S, Pinto Y, Jaitin DA, Viukov S, Rais Y, Krupalnik V,
            Chomsky E, Zerbib M, Maza I, Rechavi Y, Massarwa R, Hanna S, Amit I,
            Levanon EY, Amariglio N, Stern-Ginossar N, Novershtern N, Rechavi G,
            Hanna JH
  TITLE     Stem cells. m6A mRNA methylation facilitates resolution of naive 
            pluripotency toward differentiation.
  JOURNAL   Science. 2015 Feb 27;347(6225):1002-6. doi: 10.1126/science.1261417.
            Epub 2015 Jan 1.
  PUBMED    25569111
REFERENCE   2  (bases 1 to 799)
  AUTHORS   .
  TITLE     Direct Submission
  JOURNAL   Exported Dec 15, 2025 from SnapGene Server 8.0.1
            https://www.snapgene.com
COMMENT     SGRef: number: 1; type: "Journal Article"; doi: 
            "10.1126/science.1261417"; journalName: "Science"; date: 
            "2015-02-27- 27"; volume: "347"; issue: "6225"; pages: "1002-6"
COMMENT     Sequence Label: px335 Mettl14 sgRNA #1
FEATURES             Location/Qualifiers
     source          1..799
                     /mol_type="genomic DNA"
                     /organism="unspecified"
     CDS             55..81
                     /codon_start=1
                     /product="HA (human influenza hemagglutinin) epitope tag"
                     /label=HA
                     /translation="YPYDVPDYA"
     CDS             85..105
                     /codon_start=1
                     /product="nuclear localization signal of SV40 (simian virus
                     40) large T antigen"
                     /label=SV40 NLS
                     /translation="PKKKRKV"
ORIGIN
        1 cctggagcac ctgcctgaaa tcactttttt tcaggttgga ccggtgccac catgtaccca
       61 tacgatgttc cagattacgc ttcgccgaag aaaaagcgca aggtcgaagc gtccgacaag
      121 aagtacagca tcggcctggc catcggcacc aactctgtgg gctgggccgt gatcaccgac
      181 gagtacaagg tgcccagcaa gaaattcaag gtgctgggca acaccgaccg gcacagcatc
      241 aagaagaacc tgatcggagc cctgctgttc gacagcggcg aaacagccga ggccacccgg
      301 ctgaagagaa ccgccagaag aagatacacc agacggaaga accggatctg ctatctgcaa
      361 gagatcttca gcaacgagat ggccaaggtg gacgacagct tcttccacag actggaagag
      421 tccttcctgg tggaagagga taagaagcac gagcggcacc ccatcttcgg caacatcgtg
      481 gacgaggtgg cctaccacga gaagtacccc accatctacc acctgagaaa gaaactggtg
      541 gacagcaccg acaaggccga cctgcggctg atctatctgg ccctggccca catgatcaag
      601 ttccggggcc acttcctgat cgagggcgac ctgaaccccg acaacagcga cgtggacaag
      661 ctgttcatcc agctggtgca gacctacaac cagctgttcg aggaaaaccc catcaacgcc
      721 agcggcgtgg acgccaaggc catcctgtct gccagactga gcaagagcag acggctggaa
      781 aatctgatcg cccagctgc
//
