LOCUS       sequence-101812-         580 bp    DNA     linear   UNA 15-DEC-2025
DEFINITION  encodes sgRNA sequence for targeting mouse Mettl14 locus 
            (Cas9-Nickase strategy).
ACCESSION   .
VERSION     .
KEYWORDS    .
SOURCE      natural DNA sequence
  ORGANISM  unspecified
REFERENCE   1  (bases 1 to 580)
  AUTHORS   Geula S, Moshitch-Moshkovitz S, Dominissini D, Mansour AA, Kol N, 
            Salmon-Divon M, Hershkovitz V, Peer E, Mor N, Manor YS, Ben-Haim MS,
            Eyal E, Yunger S, Pinto Y, Jaitin DA, Viukov S, Rais Y, Krupalnik V,
            Chomsky E, Zerbib M, Maza I, Rechavi Y, Massarwa R, Hanna S, Amit I,
            Levanon EY, Amariglio N, Stern-Ginossar N, Novershtern N, Rechavi G,
            Hanna JH
  TITLE     Stem cells. m6A mRNA methylation facilitates resolution of naive 
            pluripotency toward differentiation.
  JOURNAL   Science. 2015 Feb 27;347(6225):1002-6. doi: 10.1126/science.1261417.
            Epub 2015 Jan 1.
  PUBMED    25569111
REFERENCE   2  (bases 1 to 580)
  AUTHORS   .
  TITLE     Direct Submission
  JOURNAL   Exported Dec 15, 2025 from SnapGene Server 8.0.1
            https://www.snapgene.com
COMMENT     SGRef: number: 1; type: "Journal Article"; doi: 
            "10.1126/science.1261417"; journalName: "Science"; date: 
            "2015-02-27- 27"; volume: "347"; issue: "6225"; pages: "1002-6"
COMMENT     Sequence Label: px335 Mettl14 sgRNA #1
FEATURES             Location/Qualifiers
     source          1..580
                     /mol_type="genomic DNA"
                     /organism="unspecified"
     primer_bind     118..137
                     /label=LKO.1 5'
                     /note="Human U6 promoter, forward primer"
     misc_RNA        217..292
                     /label=gRNA scaffold
                     /note="guide RNA scaffold for the Streptococcus pyogenes 
                     CRISPR/Cas9 system"
ORIGIN
        1 agagagataa ttggaattaa tttgactgta aacacaaaga tattagtaca aaatacgtga
       61 cgtagaaagt aataatttct tgggtagttt gcagttttaa aattatgttt taaaatggac
      121 tatcatatgc ttaccgtaac ttgaaagtat ttcgatttct tggctttata tatcttgtgg
      181 aaaggacgaa acaccggcgg cagctcctag ctcagcgttt tagagctaga aatagcaagt
      241 taaaataagg ctagtccgtt atcaacttga aaaagtggca ccgagtcggt gcttttttgt
      301 tttagagcta gaaatagcaa gttaaaataa ggctagtccg tttttagcgc gtgcgccaat
      361 tctgcagaca aatggctcta gaggtacccg ttacataact tacggtaaat ggcccgcctg
      421 gctgaccgcc caacgacccc cgcccattga cgtcaatagt aacgccaata gggactttcc
      481 attgacgtca atgggtggag tatttacggt aaactgccca cttggcagta catcaagtgt
      541 atcatatgcc aagtacgccc cctattgacg tcaatgacgg
//
