LOCUS       sequence-101819-         698 bp    DNA     linear   UNA 15-DEC-2025
DEFINITION  Bacterial expression for structure determination; may not be full 
            ORF.
ACCESSION   .
VERSION     .
KEYWORDS    .
SOURCE      natural DNA sequence
  ORGANISM  unspecified
REFERENCE   1  (bases 1 to 698)
  TITLE     SGC Toronto Q2 2014
  JOURNAL   Unpublished
REFERENCE   2  (bases 1 to 698)
  AUTHORS   .
  TITLE     Direct Submission
  JOURNAL   Exported Dec 15, 2025 from SnapGene Server 8.0.1
            https://www.snapgene.com
COMMENT     SGRef: number: 1; type: "Journal Article"; journalName: 
            "Unpublished"
COMMENT     Sequence Label: 4QQ6
FEATURES             Location/Qualifiers
     source          1..698
                     /mol_type="genomic DNA"
                     /organism="unspecified"
     CDS             18..35
                     /codon_start=1
                     /product="6xHis affinity tag"
                     /label=6xHis
                     /translation="HHHHHH"
     CDS             48..68
                     /codon_start=1
                     /product="tobacco etch virus (TEV) protease recognition and
                     cleavage site"
                     /label=TEV site
                     /translation="ENLYFQG"
     CDS             296..313
                     /codon_start=1
                     /product="6xHis affinity tag"
                     /label=6xHis
                     /translation="HHHHHH"
     primer_bind     complement(366..384)
                     /label=T7 Term
                     /note="T7 terminator, reverse primer"
     terminator      380..427
                     /label=T7 terminator
                     /note="transcription terminator for bacteriophage T7 RNA 
                     polymerase"
     primer_bind     complement(551..570)
                     /label=F1ori-R
                     /note="F1 origin, reverse primer"
ORIGIN
        1 gaaggagata taccatgcat catcatcatc atcacagcag cggcagagaa aacttgtatt
       61 tccagggcaa gaagaatact gcagcttcct tacaacagtg gaaagttggg gacaaatgtt
      121 ctgccatttg gtcagaagac ggttgcattt acccagctac cattgcttca attgatttta
      181 agagagaaac ctgtgttgtg gtttacactg gatatggaaa tagagaggag caaaatctgt
      241 ccgatctact ttccccaatc tgtgaatgat gacgaagctt gcggccgcac tcgagcacca
      301 ccaccaccac cactgagatc cggctgctaa caaagcccga aaggaagctg agttggctgc
      361 tgccaccgct gagcaataac tagcataacc ccttggggcc tctaaacggg tcttgagggg
      421 ttttttgctg aaaggaggaa ctatatccgg attggcgaat gggacgcgcc ctgtagcggc
      481 gcattaagcg cggcgggtgt ggtggttacg cgcagcgtga ccgctacact tgccagcgcc
      541 ctagcgcccg ctcctttcgc tttcttccct tcctttctcg ccacgttcgc cggctttccc
      601 cgtcaagctc taaatcgggg gctcccttta gggttccgat ttagtgcttt acggcacctc
      661 gaccccaaaa aacttgatta gggtgatggt tcacgtag
//
