LOCUS       sequence-101820-         824 bp    DNA     linear   UNA 15-DEC-2025
DEFINITION  Bacterial expression for structure determination; may not be full 
            ORF.
ACCESSION   .
VERSION     .
KEYWORDS    .
SOURCE      natural DNA sequence
  ORGANISM  unspecified
REFERENCE   1  (bases 1 to 824)
  TITLE     SGC Toronto Q2 2014
  JOURNAL   Unpublished
REFERENCE   2  (bases 1 to 824)
  AUTHORS   .
  TITLE     Direct Submission
  JOURNAL   Exported Dec 15, 2025 from SnapGene Server 8.0.1
            https://www.snapgene.com
COMMENT     SGRef: number: 1; type: "Journal Article"; journalName: 
            "Unpublished"
COMMENT     Sequence Label: 4QQ6
FEATURES             Location/Qualifiers
     source          1..824
                     /mol_type="genomic DNA"
                     /organism="unspecified"
     promoter        complement(38..115)
                     /gene="lacI"
                     /label=lacI promoter
     primer_bind     321..340
                     /label=pBRrevBam
                     /note="pBR322 vectors, tet region, downstream of BamHI, 
                     reverse primer"
     primer_bind     424..443
                     /label=T7
                     /note="T7 promoter, forward primer"
     promoter        424..442
                     /label=T7 promoter
                     /note="promoter for bacteriophage T7 RNA polymerase"
     protein_bind    443..467
                     /label=lac operator
                     /bound_moiety="lac repressor encoded by lacI"
                     /note="The lac repressor binds to the lac operator to 
                     inhibit transcription in E. coli. This inhibition can be 
                     relieved by adding lactose or 
                     isopropyl-beta-D-thiogalactopyranoside (IPTG)."
     RBS             482..504
                     /note="efficient ribosome binding site from bacteriophage 
                     T7 gene 10 (Olins and Rangwala, 1989)"
     CDS             514..531
                     /codon_start=1
                     /product="6xHis affinity tag"
                     /label=6xHis
                     /translation="HHHHHH"
     CDS             544..564
                     /codon_start=1
                     /product="tobacco etch virus (TEV) protease recognition and
                     cleavage site"
                     /label=TEV site
                     /translation="ENLYFQG"
     CDS             792..809
                     /codon_start=1
                     /product="6xHis affinity tag"
                     /label=6xHis
                     /translation="HHHHHH"
ORIGIN
        1 catactctgc gacatcgtat aacgttactg gtttcacatt caccaccctg aattgactct
       61 cttccgggcg ctatcatgcc ataccgcgaa aggttttgcg ccattcgatg gtgtccggga
      121 tctcgacgct ctcccttatg cgactcctgc attaggaagc agcccagtag taggttgagg
      181 ccgttgagca ccgccgccgc aaggaatggt gcatgcaagg agatggcgcc caacagtccc
      241 ccggccacgg ggcctgccac catacccacg ccgaaacaag cgctcatgag cccgaagtgg
      301 cgagcccgat cttccccatc ggtgatgtcg gcgatatagg cgccagcaac cgcacctgtg
      361 gcgccggtga tgccggccac gatgcgtccg gcgtagagga tcgagatctc gatcccgcga
      421 aattaatacg actcactata ggggaattgt gagcggataa caattcccct ctagaaataa
      481 ttttgtttaa ctttaagaag gagatatacc atgcatcatc atcatcatca cagcagcggc
      541 agagaaaact tgtatttcca gggcaagaag aatactgcag cttccttaca acagtggaaa
      601 gttggggaca aatgttctgc catttggtca gaagacggtt gcatttaccc agctaccatt
      661 gcttcaattg attttaagag agaaacctgt gttgtggttt acactggata tggaaataga
      721 gaggagcaaa atctgtccga tctactttcc ccaatctgtg aatgatgacg aagcttgcgg
      781 ccgcactcga gcaccaccac caccaccact gagatccggc tgct
//
