LOCUS       sequence-101823-         774 bp    DNA     linear   UNA 15-DEC-2025
DEFINITION  Bacterial expression for structure determination; may not be full 
            ORF.
ACCESSION   .
VERSION     .
KEYWORDS    .
SOURCE      natural DNA sequence
  ORGANISM  unspecified
REFERENCE   1  (bases 1 to 774)
  TITLE     SGC Toronto Q2 2014
  JOURNAL   Unpublished
REFERENCE   2  (bases 1 to 774)
  AUTHORS   .
  TITLE     Direct Submission
  JOURNAL   Exported Dec 15, 2025 from SnapGene Server 8.0.1
            https://www.snapgene.com
COMMENT     SGRef: number: 1; type: "Journal Article"; journalName: 
            "Unpublished"
COMMENT     Sequence Label: 4QL1
FEATURES             Location/Qualifiers
     source          1..774
                     /mol_type="genomic DNA"
                     /organism="unspecified"
     RBS             2..24
                     /note="efficient ribosome binding site from bacteriophage 
                     T7 gene 10 (Olins and Rangwala, 1989)"
     CDS             34..51
                     /codon_start=1
                     /product="6xHis affinity tag"
                     /label=6xHis
                     /translation="HHHHHH"
     CDS             64..84
                     /codon_start=1
                     /product="tobacco etch virus (TEV) protease recognition and
                     cleavage site"
                     /label=TEV site
                     /translation="ENLYFQG"
ORIGIN
        1 ttttgtttaa ctttaagaag gagatatacc atgcatcatc atcatcatca cagcagcggc
       61 agagaaaact tgtatttcca gggcactcag agcaagccta cacctgtgaa gccaaactat
      121 gctctaaagt tcacccttgc tggccacacc aaagcagtgt cctccgtgaa attcagcccg
      181 aatggagagt ggctggcaag ttcatctgct gataaactta ttaaaatttg gggcgcgtat
      241 gatgggaaat ttgagaaaac catatctggt cacaagctgg gaatatccga tgtagcctgg
      301 tcgtcagatt ctaaccttct tgtttctgcc tcagatgaca aaaccttgaa gatatgggac
      361 gtgagctcgg gcaagtgtct gaaaaccctg aagggacaca gtaattatgt cttttgctgc
      421 aacttcaatc cccagtccaa ccttattgtc tcaggatcct ttgacgaaag cgtgaggata
      481 tgggatgtga aaacagggaa gtgcctcaag actttgccag ctcactcgga tccagtctcg
      541 gccgttcatt ttaatcgtga tggatccttg atagtttcaa gtagctatga tggtctctgt
      601 cgcatctggg acaccgcctc gggccagtgc ctgaagacgc tcatcgatga cgacaacccc
      661 cccgtgtctt ttgtgaagtt ctccccgaac ggcaaataca tcctggccgc cacgctggac
      721 aacactctga agctctggga ctacagcaag gggaagtgcc tgaagacgta cact
//
