LOCUS       sequence-101825-         706 bp    DNA     linear   UNA 15-DEC-2025
DEFINITION  Bacterial expression for structure determination; may not be full 
            ORF.
ACCESSION   .
VERSION     .
KEYWORDS    .
SOURCE      natural DNA sequence
  ORGANISM  unspecified
REFERENCE   1  (bases 1 to 706)
  TITLE     SGC Toronto Q2 2014
  JOURNAL   Unpublished
REFERENCE   2  (bases 1 to 706)
  AUTHORS   .
  TITLE     Direct Submission
  JOURNAL   Exported Dec 15, 2025 from SnapGene Server 8.0.1
            https://www.snapgene.com
COMMENT     SGRef: number: 1; type: "Journal Article"; journalName: 
            "Unpublished"
COMMENT     Sequence Label: 4P7A
FEATURES             Location/Qualifiers
     source          1..706
                     /mol_type="genomic DNA"
                     /organism="unspecified"
     CDS             24..41
                     /codon_start=1
                     /product="6xHis affinity tag"
                     /label=6xHis
                     /translation="HHHHHH"
     CDS             54..74
                     /codon_start=1
                     /product="tobacco etch virus (TEV) protease recognition and
                     cleavage site"
                     /label=TEV site
                     /translation="ENLYFQG"
ORIGIN
        1 ctttaagaag gagatatacc atgcatcatc atcatcatca cagcagcggc agagaaaact
       61 tgtatttcca gggcatgtcg tttgtggcag gggttattcg gcggctggac gagacagtgg
      121 tgaaccgcat cgcggcgggg gaagttatcc agcggccagc taatgctatc aaagagatga
      181 ttgagaactg tttagatgca aaatccacaa gtattcaagt gattgttaaa gagggaggcc
      241 tgaagttgat tcagatccaa gacaatggca ccgggatcag gaaagaagat ctggatattg
      301 tatgtgaaag gttcactact agtaaactgc agtcctttga ggatttagcc agtatttcta
      361 cctatggctt tcgaggtgag gctttggcca gcataagcca tgtggctcat gttactatta
      421 caacgaaaac agctgatgga aagtgtgcat acagagcaag ttactcagat ggaaaactga
      481 aagcccctcc taaaccatgt gctggcaatc aagggaccca gatcacggtg gaggaccttt
      541 tttacaacat agccacgagg agaaaagctt taaaaaatcc aagtgaagaa tatgggaaaa
      601 ttttggaagt tgttggcagg tattcagtac acaatgcagg cattagtttc tcagttaaaa
      661 aacaaggaga gacagtagct gatgttagga cactacccaa tgcctc
//
