LOCUS       sequence-101827-         705 bp    DNA     linear   UNA 15-DEC-2025
DEFINITION  Bacterial expression for structure determination; may not be full 
            ORF.
ACCESSION   .
VERSION     .
KEYWORDS    .
SOURCE      natural DNA sequence
  ORGANISM  unspecified
REFERENCE   1  (bases 1 to 705)
  TITLE     SGC Toronto Q2 2014
  JOURNAL   Unpublished
REFERENCE   2  (bases 1 to 705)
  AUTHORS   .
  TITLE     Direct Submission
  JOURNAL   Exported Dec 15, 2025 from SnapGene Server 8.0.1
            https://www.snapgene.com
COMMENT     SGRef: number: 1; type: "Journal Article"; journalName: 
            "Unpublished"
COMMENT     Sequence Label: 4QQD
FEATURES             Location/Qualifiers
     source          1..705
                     /mol_type="genomic DNA"
                     /organism="unspecified"
     CDS             24..41
                     /codon_start=1
                     /product="6xHis affinity tag"
                     /label=6xHis
                     /translation="HHHHHH"
     CDS             54..74
                     /codon_start=1
                     /product="tobacco etch virus (TEV) protease recognition and
                     cleavage site"
                     /label=TEV site
                     /translation="ENLYFQG"
     CDS             584..601
                     /codon_start=1
                     /product="6xHis affinity tag"
                     /label=6xHis
                     /translation="HHHHHH"
ORIGIN
        1 ctttaagaag gagatatacc atgcatcatc atcatcatca cagcagcggc agagaaaact
       61 tgtatttcca gggcatgtgg gatgagacgg aattggggct gtacaaggtc aatgagtacg
      121 tcgatgctcg ggacacgaac atgggggcgt ggtttgaggc gcaggtggtc agggtgacgc
      181 ggaaggcccc ctcccgggac gagccctgca gctccacgtc caggccggcg ctggaggagg
      241 acgtcattta ccacgtgaaa tacgacgact acccggagaa cggcgtggtc cagatgaact
      301 ccagggacgt ccgagcgcgc gcccgcacca tcatcaagtg gcaggacctg gaggtgggcc
      361 aggtggtcat gctcaactac aaccccgaca accccaagga gcggggcttc tggtacgacg
      421 cggagatctc caggaagcgc gagaccagga cggcgcggga actctacgcc aacgtggtgc
      481 tgggggatga ttctctgaac gactgtcgga tcatcttcgt ggacgaagtc ttcaagattg
      541 agcggccggg tgaatgatga cgaagcttgc ggccgcactc gagcaccacc accaccacca
      601 ctgagatccg gctgctaaca aagcccgaaa ggaagctgag ttggctgctg ccaccgctga
      661 gcaataaact agcataaccc cttggggcct ctaaacgggt cttga
//
