LOCUS       sequence-101828-         735 bp    DNA     linear   UNA 15-DEC-2025
DEFINITION  Bacterial expression for structure determination; may not be full 
            ORF.
ACCESSION   .
VERSION     .
KEYWORDS    .
SOURCE      natural DNA sequence
  ORGANISM  unspecified
REFERENCE   1  (bases 1 to 735)
  TITLE     SGC Toronto Q2 2014
  JOURNAL   Unpublished
REFERENCE   2  (bases 1 to 735)
  AUTHORS   .
  TITLE     Direct Submission
  JOURNAL   Exported Dec 15, 2025 from SnapGene Server 8.0.1
            https://www.snapgene.com
COMMENT     SGRef: number: 1; type: "Journal Article"; journalName: 
            "Unpublished"
COMMENT     Sequence Label: 4QQD
FEATURES             Location/Qualifiers
     source          1..735
                     /mol_type="genomic DNA"
                     /organism="unspecified"
     primer_bind     4..23
                     /label=pBRrevBam
                     /note="pBR322 vectors, tet region, downstream of BamHI, 
                     reverse primer"
     primer_bind     107..126
                     /label=T7
                     /note="T7 promoter, forward primer"
     promoter        107..125
                     /label=T7 promoter
                     /note="promoter for bacteriophage T7 RNA polymerase"
     protein_bind    126..150
                     /label=lac operator
                     /bound_moiety="lac repressor encoded by lacI"
                     /note="The lac repressor binds to the lac operator to 
                     inhibit transcription in E. coli. This inhibition can be 
                     relieved by adding lactose or 
                     isopropyl-beta-D-thiogalactopyranoside (IPTG)."
     RBS             165..187
                     /note="efficient ribosome binding site from bacteriophage 
                     T7 gene 10 (Olins and Rangwala, 1989)"
     CDS             197..214
                     /codon_start=1
                     /product="6xHis affinity tag"
                     /label=6xHis
                     /translation="HHHHHH"
     CDS             227..247
                     /codon_start=1
                     /product="tobacco etch virus (TEV) protease recognition and
                     cleavage site"
                     /label=TEV site
                     /translation="ENLYFQG"
ORIGIN
        1 atcggtgatg tcggcgatat aggcgccagc aaccgcacct gtggcgccgg tgatgccggc
       61 cacgatgcgt ccggcgtaga ggatcgagat ctcgatcccg cgaaattaat acgactcact
      121 ataggggaat tgtgagcgga taacaattcc cctctagaaa taattttgtt taactttaag
      181 aaggagatat accatgcatc atcatcatca tcacagcagc ggcagagaaa acttgtattt
      241 ccagggcatg tgggatgaga cggaattggg gctgtacaag gtcaatgagt acgtcgatgc
      301 tcgggacacg aacatggggg cgtggtttga ggcgcaggtg gtcagggtga cgcggaaggc
      361 cccctcccgg gacgagccct gcagctccac gtccaggccg gcgctggagg aggacgtcat
      421 ttaccacgtg aaatacgacg actacccgga gaacggcgtg gtccagatga actccaggga
      481 cgtccgagcg cgcgcccgca ccatcatcaa gtggcaggac ctggaggtgg gccaggtggt
      541 catgctcaac tacaaccccg acaaccccaa ggagcggggc ttctggtacg acgcggagat
      601 ctccaggaag cgcgagacca ggacggcgcg ggaactctac gccaacgtgg tgctggggga
      661 tgattctctg aacgactgtc ggatcatctt cgtggacgaa gtcttcaaga ttgagcggcc
      721 gggtgaatga tgacg
//
