LOCUS       sequence-101829-         652 bp    DNA     linear   UNA 15-DEC-2025
DEFINITION  Bacterial expression for structure determination; may not be full 
            ORF.
ACCESSION   .
VERSION     .
KEYWORDS    .
SOURCE      natural DNA sequence
  ORGANISM  unspecified
REFERENCE   1  (bases 1 to 652)
  TITLE     SGC Toronto Q2 2014
  JOURNAL   Unpublished
REFERENCE   2  (bases 1 to 652)
  AUTHORS   .
  TITLE     Direct Submission
  JOURNAL   Exported Dec 15, 2025 from SnapGene Server 8.0.1
            https://www.snapgene.com
COMMENT     SGRef: number: 1; type: "Journal Article"; journalName: 
            "Unpublished"
COMMENT     Sequence Label: 4QQG
FEATURES             Location/Qualifiers
     source          1..652
                     /mol_type="genomic DNA"
                     /organism="unspecified"
     CDS             17..34
                     /codon_start=1
                     /product="6xHis affinity tag"
                     /label=6xHis
                     /translation="HHHHHH"
     CDS             47..67
                     /codon_start=1
                     /product="tobacco etch virus (TEV) protease recognition and
                     cleavage site"
                     /label=TEV site
                     /translation="ENLYFQG"
     CDS             337..354
                     /codon_start=1
                     /product="6xHis affinity tag"
                     /label=6xHis
                     /translation="HHHHHH"
     primer_bind     complement(407..425)
                     /label=T7 Term
                     /note="T7 terminator, reverse primer"
     terminator      421..468
                     /label=T7 terminator
                     /note="transcription terminator for bacteriophage T7 RNA 
                     polymerase"
     primer_bind     complement(592..611)
                     /label=F1ori-R
                     /note="F1 origin, reverse primer"
ORIGIN
        1 aaggagatat accatgcatc atcatcatca tcactgcagc ggcagagaaa acttgtattt
       61 ccagggcatg gcggaggtgg gggagataat cgagggctgc cgcctacccg tgctgcggcg
      121 gaaccaggac aacgaagatg agtggcccct ggccgagatc ctgagcgtga aggacatcag
      181 tggccggaag cttttctacg tccattacat tgacttcaac aaacgtctgg atgaatgggt
      241 gacgcatgag cggctggacc taaagaagat ccagttcccc aagaaagagg ccaagacccc
      301 cactaagtga tgacgaagct tgcggccgca ctcgagcacc accaccacca ccactgagat
      361 ccggctgcta acaaagcccg aaaggaagct gagttggctg ctgccaccgc tgagcaataa
      421 ctagcataac cccttggggc ctctaaacgg gtcttgaggg gttttttgct gaaaggagga
      481 actatatccg gattggcgaa tgggacgcgc cctgtagcgg cgcattaagc gcggcgggtg
      541 tggtggttac gcgcagcgtg accgctacac ttgccagcgc cctagcgccc gctcctttcg
      601 ctttcttccc ttcctttctc gccacgttcg ccggctttcc ccgtcaagct ct
//
