LOCUS       sequence-101834-         706 bp    DNA     linear   UNA 15-DEC-2025
DEFINITION  Bacterial expression for structure determination; may not be full 
            ORF.
ACCESSION   .
VERSION     .
KEYWORDS    .
SOURCE      natural DNA sequence
  ORGANISM  unspecified
REFERENCE   1  (bases 1 to 706)
  TITLE     SGC Toronto Q2 2014
  JOURNAL   Unpublished
REFERENCE   2  (bases 1 to 706)
  AUTHORS   .
  TITLE     Direct Submission
  JOURNAL   Exported Dec 15, 2025 from SnapGene Server 8.0.1
            https://www.snapgene.com
COMMENT     SGRef: number: 1; type: "Journal Article"; journalName: 
            "Unpublished"
COMMENT     Sequence Label: 4QQI
FEATURES             Location/Qualifiers
     source          1..706
                     /mol_type="genomic DNA"
                     /organism="unspecified"
     CDS             30..47
                     /codon_start=1
                     /product="6xHis affinity tag"
                     /label=6xHis
                     /translation="HHHHHH"
     CDS             60..80
                     /codon_start=1
                     /product="tobacco etch virus (TEV) protease recognition and
                     cleavage site"
                     /label=TEV site
                     /translation="ENLYFQG"
     CDS             626..643
                     /codon_start=1
                     /product="6xHis affinity tag"
                     /label=6xHis
                     /translation="HHHHHH"
ORIGIN
        1 gtttaacttt aagaaggaga tataccatgc atcatcatca tcatcacagc agcggcagag
       61 aaaacttgta tttccagggc tctaccacac ctctgttagc aaattctttg tctgttcacc
      121 agttggctgc tcagggagag atgctctatc tggctactcg tatcgaacaa gaaaatgtta
      181 tcaatcacac ggatgaagaa ggatttactc ctctgatgtg ggctgcagca cacgggcaaa
      241 tagctgtggt agagttccta cttcagaatg gtgctgatcc ccaactttta ggaaaaggtc
      301 gagaaagtgc actgtcgttg gcctgtagta aaggctacac agatattgtc aaaatgctgc
      361 ttgattgtgg agttgatgta aatgaatatg attggaatgg aggaacacct ctgctttatg
      421 ctgtacatgg aaatcatgtg aaatgtgtaa agatgctctt agaaagtggg gctgatccaa
      481 caattgaaac tgactctgga tataattcta tggatctagc tgtagcccta ggctatagaa
      541 gtgttcaaca ggttattgag tcacatttgt tgaagctgct tcaaaatatc aaggagtgat
      601 gacgaagctt gcggccgcac tcgagcacca ccaccaccac cactgagatc cggctgctaa
      661 caaaagcccg aaaaggaagc tgagttggct gctgccaccg ctgagc
//
