LOCUS       sequence-101835-         726 bp    DNA     linear   UNA 15-DEC-2025
DEFINITION  Bacterial expression for structure determination; may not be full 
            ORF.
ACCESSION   .
VERSION     .
KEYWORDS    .
SOURCE      natural DNA sequence
  ORGANISM  unspecified
REFERENCE   1  (bases 1 to 726)
  TITLE     SGC Toronto Q2 2014
  JOURNAL   Unpublished
REFERENCE   2  (bases 1 to 726)
  AUTHORS   .
  TITLE     Direct Submission
  JOURNAL   Exported Dec 15, 2025 from SnapGene Server 8.0.1
            https://www.snapgene.com
COMMENT     SGRef: number: 1; type: "Journal Article"; journalName: 
            "Unpublished"
COMMENT     Sequence Label: 4QQI
FEATURES             Location/Qualifiers
     source          1..726
                     /mol_type="genomic DNA"
                     /organism="unspecified"
     primer_bind     8..27
                     /label=T7
                     /note="T7 promoter, forward primer"
     promoter        8..26
                     /label=T7 promoter
                     /note="promoter for bacteriophage T7 RNA polymerase"
     protein_bind    27..51
                     /label=lac operator
                     /bound_moiety="lac repressor encoded by lacI"
                     /note="The lac repressor binds to the lac operator to 
                     inhibit transcription in E. coli. This inhibition can be 
                     relieved by adding lactose or 
                     isopropyl-beta-D-thiogalactopyranoside (IPTG)."
     RBS             66..88
                     /note="efficient ribosome binding site from bacteriophage 
                     T7 gene 10 (Olins and Rangwala, 1989)"
     CDS             98..115
                     /codon_start=1
                     /product="6xHis affinity tag"
                     /label=6xHis
                     /translation="HHHHHH"
     CDS             128..148
                     /codon_start=1
                     /product="tobacco etch virus (TEV) protease recognition and
                     cleavage site"
                     /label=TEV site
                     /translation="ENLYFQG"
     CDS             694..711
                     /codon_start=1
                     /product="6xHis affinity tag"
                     /label=6xHis
                     /translation="HHHHHH"
ORIGIN
        1 gcgaaattaa tacgactcac tataggggaa ttgtgagcgg ataacaattc ccctctagaa
       61 ataattttgt ttaactttaa gaaggagata taccatgcat catcatcatc atcacagcag
      121 cggcagagaa aacttgtatt tccagggctc taccacacct ctgttagcaa attctttgtc
      181 tgttcaccag ttggctgctc agggagagat gctctatctg gctactcgta tcgaacaaga
      241 aaatgttatc aatcacacgg atgaagaagg atttactcct ctgatgtggg ctgcagcaca
      301 cgggcaaata gctgtggtag agttcctact tcagaatggt gctgatcccc aacttttagg
      361 aaaaggtcga gaaagtgcac tgtcgttggc ctgtagtaaa ggctacacag atattgtcaa
      421 aatgctgctt gattgtggag ttgatgtaaa tgaatatgat tggaatggag gaacacctct
      481 gctttatgct gtacatggaa atcatgtgaa atgtgtaaag atgctcttag aaagtggggc
      541 tgatccaaca attgaaactg actctggata taattctatg gatctagctg tagccctagg
      601 ctatagaagt gttcaacagg ttattgagtc acatttgttg aagctgcttc aaaatatcaa
      661 ggagtgatga cgaagcttgc ggccgcactc gagcaccacc accaccacca ctgagatccg
      721 gctgct
//
