LOCUS       sequence-101839-         756 bp    DNA     linear   UNA 15-DEC-2025
DEFINITION  Bacterial expression for structure determination; may not be full 
            ORF.
ACCESSION   .
VERSION     .
KEYWORDS    .
SOURCE      natural DNA sequence
  ORGANISM  unspecified
REFERENCE   1  (bases 1 to 756)
  TITLE     SGC Toronto Q2 2014
  JOURNAL   Unpublished
REFERENCE   2  (bases 1 to 756)
  AUTHORS   .
  TITLE     Direct Submission
  JOURNAL   Exported Dec 15, 2025 from SnapGene Server 8.0.1
            https://www.snapgene.com
COMMENT     SGRef: number: 1; type: "Journal Article"; journalName: 
            "Unpublished"
COMMENT     Sequence Label: 4QQ4
FEATURES             Location/Qualifiers
     source          1..756
                     /mol_type="genomic DNA"
                     /organism="unspecified"
     primer_bind     267..286
                     /label=pBRrevBam
                     /note="pBR322 vectors, tet region, downstream of BamHI, 
                     reverse primer"
     primer_bind     370..389
                     /label=T7
                     /note="T7 promoter, forward primer"
     promoter        370..388
                     /label=T7 promoter
                     /note="promoter for bacteriophage T7 RNA polymerase"
     protein_bind    389..413
                     /label=lac operator
                     /bound_moiety="lac repressor encoded by lacI"
                     /note="The lac repressor binds to the lac operator to 
                     inhibit transcription in E. coli. This inhibition can be 
                     relieved by adding lactose or 
                     isopropyl-beta-D-thiogalactopyranoside (IPTG)."
     RBS             428..450
                     /note="efficient ribosome binding site from bacteriophage 
                     T7 gene 10 (Olins and Rangwala, 1989)"
     CDS             460..477
                     /codon_start=1
                     /product="6xHis affinity tag"
                     /label=6xHis
                     /translation="HHHHHH"
     CDS             490..510
                     /codon_start=1
                     /product="tobacco etch virus (TEV) protease recognition and
                     cleavage site"
                     /label=TEV site
                     /translation="ENLYFQG"
     CDS             723..740
                     /codon_start=1
                     /product="6xHis affinity tag"
                     /label=6xHis
                     /translation="HHHHHH"
ORIGIN
        1 gactctcttc cgggcgctat catgccatac cgcgaaaggt tttgcgccat tcgatggtgt
       61 ccgggatctc gacgctctcc cttatgcgac tcctgcatta ggaagcagcc cagtagtagg
      121 ttgaggccgt tgagcaccgc cgccgcaagg aatggtgcat gcaaggagat ggcgcccaac
      181 agtcccccgg ccacggggcc tgccaccata cccacgccga aacaagcgct catgagcccg
      241 aagtggcgag cccgatcttc cccatcggtg atgtcggcga tataggcgcc agcaaccgca
      301 cctgtggcgc cggtgatgcc ggccacgatg cgtccggcgt agaggatcga gatctcgatc
      361 ccgcgaaatt aatacgactc actatagggg aattgtgagc ggataacaat tcccctctag
      421 aaataatttt gtttaacttt aagaaggaga tataccatgc atcatcatca tcatcacagc
      481 agcggcagag aaaacttgta tttccagggc gaagatatac agaagcgtcc tgatcagaca
      541 tgggttcagt gtgatgcctg tctaaagtgg cggaaattac ctgatgggat ggatcaactt
      601 cctgaaaaat ggtattgctc caataaccct gacccacagt tcagaaattg tgaggttcca
      661 gaagaacctg aagatgagga tttggtacat ccctgatgac gaagcttgcg gccgcactcg
      721 agcaccacca ccaccaccac tgagatcccg gctgct
//
