LOCUS       sequence-101842-         717 bp    DNA     linear   UNA 15-DEC-2025
DEFINITION  Bacterial expression for structure determination; may not be full 
            ORF.
ACCESSION   .
VERSION     .
KEYWORDS    .
SOURCE      natural DNA sequence
  ORGANISM  unspecified
REFERENCE   1  (bases 1 to 717)
  TITLE     SGC Toronto Q2 2014
  JOURNAL   Unpublished
REFERENCE   2  (bases 1 to 717)
  AUTHORS   .
  TITLE     Direct Submission
  JOURNAL   Exported Dec 15, 2025 from SnapGene Server 8.0.1
            https://www.snapgene.com
COMMENT     SGRef: number: 1; type: "Journal Article"; journalName: 
            "Unpublished"
COMMENT     Sequence Label: 2MJ8
FEATURES             Location/Qualifiers
     source          1..717
                     /mol_type="genomic DNA"
                     /organism="unspecified"
     primer_bind     224..243
                     /label=pBRrevBam
                     /note="pBR322 vectors, tet region, downstream of BamHI, 
                     reverse primer"
     primer_bind     327..346
                     /label=T7
                     /note="T7 promoter, forward primer"
     promoter        327..345
                     /label=T7 promoter
                     /note="promoter for bacteriophage T7 RNA polymerase"
     protein_bind    346..370
                     /label=lac operator
                     /bound_moiety="lac repressor encoded by lacI"
                     /note="The lac repressor binds to the lac operator to 
                     inhibit transcription in E. coli. This inhibition can be 
                     relieved by adding lactose or 
                     isopropyl-beta-D-thiogalactopyranoside (IPTG)."
     RBS             385..407
                     /note="efficient ribosome binding site from bacteriophage 
                     T7 gene 10 (Olins and Rangwala, 1989)"
     CDS             417..434
                     /codon_start=1
                     /product="6xHis affinity tag"
                     /label=6xHis
                     /translation="HHHHHH"
     CDS             447..467
                     /codon_start=1
                     /product="tobacco etch virus (TEV) protease recognition and
                     cleavage site"
                     /label=TEV site
                     /translation="ENLYFQG"
     CDS             686..703
                     /codon_start=1
                     /product="6xHis affinity tag"
                     /label=6xHis
                     /translation="HHHHHH"
ORIGIN
        1 gcgccattcg atggtgtccg ggatctcgac gctctccctt atgcgactcc tgcattagga
       61 agcagcccag tagtaggttg aggccgttga gcaccgccgc cgcaaggaat ggtgcatgca
      121 aggagatggc gcccaacagt cccccggcca cggggcctgc caccataccc acgccgaaac
      181 aagcgctcat gagcccgaag tggcgagccc gatcttcccc atcggtgatg tcggcgatat
      241 aggcgccagc aaccgcacct gtggcgccgg tgatgccggc cacgatgcgt ccggcgtaga
      301 ggatcgagat ctcgatcccg cgaaattaat acgactcact ataggggaat tgtgagcgga
      361 taacaattcc cctctagaaa taattttgtt taactttaag aaggagatat accatgcatc
      421 atcatcatca tcacagcagc ggcagagaaa acttgtattt ccagggcgct tctggggacc
      481 tttacgaggt tgaaaggatt gtagacaaga ggaagaacaa gaaaggaaaa tgggagtatc
      541 ttatccgatg gaaaggctac gggagcaccg aggacacgtg ggagccggag caccacctct
      601 tgcactgtga ggagtttatt gatgaattca atgggttgca catgtccaag gacaagtgat
      661 gacgaagctt gcggccgcac tcgagcacca ccaccaccac cactgagatc cggctgc
//
