LOCUS       sequence-101845-         847 bp    DNA     linear   UNA 15-DEC-2025
DEFINITION  Expresses human USP21 (USP domain) in E. coli..
ACCESSION   .
VERSION     .
KEYWORDS    .
SOURCE      natural DNA sequence
  ORGANISM  unspecified
REFERENCE   1  (bases 1 to 847)
  AUTHORS   Ye Y, Akutsu M, Reyes-Turcu F, Enchev RI, Wilkinson KD, Komander D
  TITLE     Polyubiquitin binding and cross-reactivity in the USP domain 
            deubiquitinase USP21.
  JOURNAL   EMBO Rep. 2011 Apr;12(4):350-7. doi: 10.1038/embor.2011.17. Epub 
            2011 Mar 11.
  PUBMED    21399617
REFERENCE   2  (bases 1 to 847)
  AUTHORS   .
  TITLE     Direct Submission
  JOURNAL   Exported Dec 15, 2025 from SnapGene Server 8.0.1
            https://www.snapgene.com
COMMENT     SGRef: number: 1; type: "Journal Article"; doi: 
            "10.1038/embor.2011.17"; journalName: "EMBO Rep"; date: "2011-04"; 
            volume: "12"; issue: "4"; pages: "350-7"
COMMENT     Sequence Label: pOPINS-USP21 (USP, aa 196-565)
FEATURES             Location/Qualifiers
     source          1..847
                     /mol_type="genomic DNA"
                     /organism="unspecified"
     CDS             33..50
                     /codon_start=1
                     /product="6xHis affinity tag"
                     /label=6xHis
                     /translation="HHHHHH"
ORIGIN
        1 ctttaagaag gagatatacc atgggtagca gccatcacca tcatcatcac gggagcgata
       61 gcgaagtgaa ccaggaagcg aaaccggaag ttaaaccgga agtgaaaccg gaaacccata
      121 ttaatctgaa agttagcgac ggcagcagcg aaatcttttt taaaattaaa aaaaccaccc
      181 cgctgcgtcg cctgatggaa gcctttgcga aacgtcaggg taaagaaatg gatagcctgc
      241 gctttctgta tgacggcatc cgtattcagg ccgatcagac cccggaagac ctggatatgg
      301 aagacaacga tattattgaa gcgcatcgcg aacagatcgg tggttctgat gacaagatgg
      361 ctcatcacac actccttctg ggctctggtc atgttggcct tcgaaacctg ggaaacacgt
      421 gcttcctgaa tgctgtgctg cagtgtctga gcagcactcg acctcttcgg gacttctgtc
      481 tgagaaggga cttccggcaa gaggtgcctg gaggaggccg agcccaagag ctcactgaag
      541 cctttgcaga tgtgattggt gccctctggc accctgactc ctgcgaagct gtgaatccta
      601 ctcgattccg agctgtcttc cagaaatatg ttccctcctt ctctggatac agccagcagg
      661 atgcccaaga gttcctgaag ctcctcatgg agcggctaca ccttgaaatc aaccgccgag
      721 gccgccgggc tccaccgata cttgccaatg gtccagttcc ctctccaccc cgccgaggag
      781 gggctctgct agaagaacct gagttaagtg atgatgaccg agccaaccta atgtggaaac
      841 gttacct
//
