LOCUS       sequence-101848-         797 bp    DNA     linear   UNA 15-DEC-2025
DEFINITION  Expresses vOTU (OTU domain from CCHFV) in E. coli..
ACCESSION   .
VERSION     .
KEYWORDS    .
SOURCE      natural DNA sequence
  ORGANISM  unspecified
REFERENCE   1  (bases 1 to 797)
  AUTHORS   Akutsu M, Ye Y, Virdee S, Chin JW, Komander D
  TITLE     Molecular basis for ubiquitin and ISG15 cross-reactivity in viral 
            ovarian tumor domains.
  JOURNAL   Proc Natl Acad Sci U S A. 2011 Feb 8;108(6):2228-33. doi: 
            10.1073/pnas.1015287108. Epub 2011 Jan 25.
  PUBMED    21266548
REFERENCE   2  (bases 1 to 797)
  AUTHORS   .
  TITLE     Direct Submission
  JOURNAL   Exported Dec 15, 2025 from SnapGene Server 8.0.1
            https://www.snapgene.com
COMMENT     SGRef: number: 1; type: "Journal Article"; doi: 
            "10.1073/pnas.1015287108"; journalName: "Proc Natl Acad Sci U S A"; 
            date: "2011-02-8- 8"; volume: "108"; issue: "6"; pages: "2228-33"
COMMENT     Sequence Label: pOPINK-vOTU (CCHFV OTU, aa 1-183)
FEATURES             Location/Qualifiers
     source          1..797
                     /mol_type="genomic DNA"
                     /organism="unspecified"
     CDS             27..44
                     /codon_start=1
                     /product="6xHis affinity tag"
                     /label=6xHis
                     /translation="HHHHHH"
     CDS             45..698
                     /codon_start=1
                     /product="glutathione S-transferase from Schistosoma 
                     japonicum"
                     /label=GST
                     /translation="MSPILGYWKIKGLVQPTRLLLEYLEEKYEEHLYERDEGDKWRNKK
                     FELGLEFPNLPYYIDGDVKLTQSMAIIRYIADKHNMLGGCPKERAEISMLEGAVLDIRY
                     GVSRIAYSKDFETLKVDFLSKLPEMLKMFEDRLCHKTYLNGDHVTHPDFMLYDALDVVL
                     YMDPMCLDAFPKLVCFKKRIEAIPQIDKYLKSSKYIAWPLQGWQATFGGGDHPPK"
     primer_bind     656..678
                     /label=pGEX 5'
                     /note="pGEX vectors, Glutathione-S-transferase, forward 
                     primer"
     CDS             717..740
                     /codon_start=1
                     /product="recognition and cleavage site for human 
                     rhinovirus 3C and PreScission proteases"
                     /label=HRV 3C site
                     /translation="LEVLFQGP"
ORIGIN
        1 ctttaagaag gagatatacc atggcacatc accatcacca tcacatgtcc cctatactag
       61 gttattggaa aattaagggc cttgtgcaac ccactcgact tcttttggaa tatcttgaag
      121 aaaaatatga agagcatttg tatgagcgcg atgaaggtga taaatggcga aacaaaaagt
      181 ttgaattggg tttggagttt cccaatcttc cttattatat tgatggtgat gttaaattaa
      241 cacagtctat ggccatcata cgttatatag ctgacaagca caacatgttg ggtggttgtc
      301 caaaagagcg tgcagagatt tcaatgcttg aaggagcggt tttggatatt agatacggtg
      361 tttcgagaat tgcatatagt aaagactttg aaactctcaa agttgatttt cttagcaagc
      421 tacctgaaat gctgaaaatg ttcgaagatc gtttatgtca taaaacatat ttaaatggtg
      481 atcatgtaac ccatcctgac ttcatgttgt atgacgctct tgatgttgtt ttatacatgg
      541 acccaatgtg cctggatgcg ttcccaaaat tagtttgttt taaaaaacgt attgaagcta
      601 tcccacaaat tgataagtac ttgaaatcca gcaagtatat agcatggcct ttgcagggct
      661 ggcaagccac gtttggtggt ggcgaccatc ctccaaaatc ggatctgagc agcggtctgg
      721 aagttctgtt tcagggcccg atggactttc tgcgttccct ggattggaca caggttattg
      781 ccggtcagta tgtgagc
//
