LOCUS       sequence-101850-         856 bp    DNA     linear   UNA 15-DEC-2025
DEFINITION  Expresses vOTU (OTU domain from CCHFV) in E. coli..
ACCESSION   .
VERSION     .
KEYWORDS    .
SOURCE      natural DNA sequence
  ORGANISM  unspecified
REFERENCE   1  (bases 1 to 856)
  AUTHORS   Akutsu M, Ye Y, Virdee S, Chin JW, Komander D
  TITLE     Molecular basis for ubiquitin and ISG15 cross-reactivity in viral 
            ovarian tumor domains.
  JOURNAL   Proc Natl Acad Sci U S A. 2011 Feb 8;108(6):2228-33. doi: 
            10.1073/pnas.1015287108. Epub 2011 Jan 25.
  PUBMED    21266548
REFERENCE   2  (bases 1 to 856)
  AUTHORS   .
  TITLE     Direct Submission
  JOURNAL   Exported Dec 15, 2025 from SnapGene Server 8.0.1
            https://www.snapgene.com
COMMENT     SGRef: number: 1; type: "Journal Article"; doi: 
            "10.1073/pnas.1015287108"; journalName: "Proc Natl Acad Sci U S A"; 
            date: "2011-02-8- 8"; volume: "108"; issue: "6"; pages: "2228-33"
COMMENT     Sequence Label: pOPINK-vOTU (CCHFV OTU, aa 1-183)
FEATURES             Location/Qualifiers
     source          1..856
                     /mol_type="genomic DNA"
                     /organism="unspecified"
     primer_bind     164..186
                     /label=pGEX 5'
                     /note="pGEX vectors, Glutathione-S-transferase, forward 
                     primer"
     CDS             225..248
                     /codon_start=1
                     /product="recognition and cleavage site for human 
                     rhinovirus 3C and PreScission proteases"
                     /label=HRV 3C site
                     /translation="LEVLFQGP"
     CDS             821..838
                     /codon_start=1
                     /product="6xHis affinity tag"
                     /label=6xHis
                     /translation="HHHHHH"
ORIGIN
        1 atcctgactt catgttgtat gacgctcttg atgttgtttt atacatggac ccaatgtgcc
       61 tggatgcgtt cccaaaatta gtttgtttta aaaaacgtat tgaagctatc ccacaaattg
      121 ataagtactt gaaatccagc aagtatatag catggccttt gcagggctgg caagccacgt
      181 ttggtggtgg cgaccatcct ccaaaatcgg atctgagcag cggtctggaa gttctgtttc
      241 agggcccgat ggactttctg cgttccctgg attggacaca ggttattgcc ggtcagtatg
      301 tgagcaaccc tcgctttaac atttccgact atttcgagat cgttcgtcag cctggtgatg
      361 ggaattgctt ctatcacagc attgccgaac tgacaatgcc gaacaaaacc gatcactcgt
      421 atcattatat caaacgcctg actgaatcag cagctcgtaa atattatcag gaggagccgg
      481 aagctcgtct ggttggtctg tctctggagg attatctgaa acgtatgctg agtgacaacg
      541 aatggggtag cactctggaa gcctctatgc tggcaaaaga aatgggcatt acgatcatca
      601 tttggaccgt tgccgcctcc gatgaagttg aagccgggat caaatttgga gatggcgacg
      661 tgtttaccgc cgttaatctg ctgcatagtg gtcagaccca ctttgatgca ctgcgtattc
      721 tgccacagtt tgaaacagac acccgtgaag cgctgtctct gatggaccgt gttattgccg
      781 tggatcagct gacaagctaa agctttctag accatttaaa caccaccacc accaccactg
      841 agatccggct gctaac
//
