LOCUS       sequence-101855-        4313 bp    DNA     circular SYN 15-DEC-2025
DEFINITION  QUAS transgenesis vector.
ACCESSION   .
VERSION     .
KEYWORDS    .
SOURCE      synthetic DNA construct
  ORGANISM  synthetic DNA construct
REFERENCE   1  (bases 1 to 4313)
  AUTHORS   Horstick EJ, Jordan DC, Bergeron SA, Tabor KM, Serpe M, Feldman B, 
            Burgess HA
  TITLE     Increased functional protein expression using nucleotide sequence 
            features enriched in highly expressed genes in zebrafish.
  JOURNAL   Nucleic Acids Res. 2015 Jan 27. pii: gkv035.
  PUBMED    25628360
REFERENCE   2  (bases 1 to 4313)
  AUTHORS   .
  TITLE     Direct Submission
  JOURNAL   Exported Dec 15, 2025 from SnapGene Server 8.0.1
            https://www.snapgene.com
COMMENT     SGRef: number: 1; type: "Journal Article"; journalName: "Nucleic 
            Acids Res. 2015 Jan 27. pii: gkv035."
COMMENT     Sequence Label: pT1QciMP
FEATURES             Location/Qualifiers
     source          1..4313
                     /mol_type="other DNA"
                     /organism="synthetic DNA construct"
     protein_bind    106..127
                     /label=CAP binding site
                     /bound_moiety="E. coli catabolite activator protein"
                     /note="CAP binding activates transcription in the presence 
                     of cAMP."
     promoter        join(142..147,148..165,166..172)
                     /label=lac promoter
                     /note="promoter for the E. coli lac operon"
     protein_bind    180..196
                     /label=lac operator
                     /bound_moiety="lac repressor encoded by lacI"
                     /note="The lac repressor binds to the lac operator to 
                     inhibit transcription in E. coli. This inhibition can be 
                     relieved by adding lactose or 
                     isopropyl-beta-D-thiogalactopyranoside (IPTG)."
     primer_bind     185..207
                     /label=M13/pUC Reverse
                     /note="In lacZ gene"
     primer_bind     204..220
                     /label=M13 rev
                     /note="common sequencing primer, one of multiple similar 
                     variants"
     primer_bind     204..220
                     /label=M13 Reverse
                     /note="In lacZ gene. Also called M13-rev"
     protein_bind    428..448
                     /gene="mutant version of attB"
                     /label=attB4
                     /bound_moiety="BP Clonase(TM)"
                     /note="core recombination site for the Gateway(R) BP 
                     reaction"
     regulatory      1323..1332
                     /label=Kozak sequence
                     /note="vertebrate consensus sequence for strong initiation 
                     of translation (Kozak, 1987)"
                     /regulatory_class="other"
     primer_bind     complement(1917..1934)
                     /label=M13 Forward
                     /note="In lacZ gene. Also called M13-F20 or M13 (-21) 
                     Forward"
     primer_bind     complement(1917..1933)
                     /label=M13 fwd
                     /note="common sequencing primer, one of multiple similar 
                     variants"
     primer_bind     complement(1926..1948)
                     /label=M13/pUC Forward
                     /note="In lacZ gene"
     primer_bind     complement(2142..2161)
                     /label=pRS-marker
                     /note="pRS vectors, use to sequence yeast selectable 
                     marker"
     primer_bind     2261..2283
                     /label=pGEX 3'
                     /note="pGEX vectors, reverse primer"
     primer_bind     complement(2321..2339)
                     /label=pBRforEco
                     /note="pBR322 vectors, upsteam of EcoRI site, forward 
                     primer"
     promoter        2407..2511
                     /gene="bla"
                     /label=AmpR promoter
     CDS             join(2512..2580,2581..3372)
                     /codon_start=1
                     /gene="bla"
                     /product="beta-lactamase"
                     /label=AmpR
                     /note="confers resistance to ampicillin, carbenicillin, and
                     related antibiotics"
                     /translation="MSIQHFRVALIPFFAAFCLPVFAHPETLVKVKDAEDQLGARVGYI
                     ELDLNSGKILESFRPEERFPMMSTFKVLLCGAVLSRIDAGQEQLGRRIHYSQNDLVEYS
                     PVTEKHLTDGMTVRELCSAAITMSDNTAANLLLTTIGGPKELTAFLHNMGDHVTRLDRW
                     EPELNEAIPNDERDTTMPVAMATTLRKLLTGELLTLASRQQLIDWMEADKVAGPLLRSA
                     LPAGWFIADKSGAGERGSRGIIAALGPDGKPSRIVVIYTTGSQATMDERNRQIAEIGAS
                     LIKHW"
     primer_bind     complement(2730..2749)
                     /label=Amp-R
                     /note="Ampicillin resistance gene, reverse primer"
     rep_origin      3543..4131
                     /direction=RIGHT
                     /label=ori
                     /note="high-copy-number ColE1/pMB1/pBR322/pUC origin of 
                     replication"
     primer_bind     4032..4051
                     /label=pBR322ori-F
                     /note="pBR322 origin, forward primer"
     primer_bind     4285..4302
                     /label=L4440
                     /note="L4440 vector, forward primer"
ORIGIN
        1 gcgcccaata cgcaaaccgc ctctccccgc gcgttggccg attcattaat gcagctggca
       61 cgacaggttt cccgactgga aagcgggcag tgagcgcaac gcaattaatg tgagttagct
      121 cactcattag gcaccccagg ctttacactt tatgcttccg gctcgtatgt tgtgtggaat
      181 tgtgagcgga taacaatttc acacaggaaa cagctatgac catgattacg ccaagcttgc
      241 atggtccctt tagccagtag cggttctagg cacgggccgt ccgggcggtg gcctggggcg
      301 gaaaactgaa ggggggcggc accggcggct cagcccttgt aatatattat atgcaccact
      361 attggtttac ttatgtcaca gtttgtaagt ttgtaacagc ctgaacctgg atcagcttag
      421 gtaatgccaa ctttgtatag aaaagttggg gtaatcgctt atcctcggat aaacaattat
      481 cctcacgggt aatcgcttat ccgctcgggt aatcgcttat cctcgggtaa tcgcttatcc
      541 ttgcaagggt cgactctaga gggtatataa tggatcccat cgcgtctcag cctcactttg
      601 agctcctcca cacgaattcg atactaagga tccttgtttg tgagtatgat gttaaatata
      661 agcaatcgac gtttgttttg tcgtttgtta aatctagcaa tgttttattt caattaaagt
      721 gttaaccggt tcatttaaag gtgtatttct gaatgagcta acgtattagc ttttaacgct
      781 agccggactg tttggttttg aggatcaata aaccaacgtt tttttctgat ttattttgct
      841 ttaaaatata aaggtataat tcgtcgtcga gaattttggg ttttgttcga gtttagaaat
      901 ttggcggcgt ttttttgtac cgcgaaacag ggcggtggtt cggtgagctt ctcaattcat
      961 ctgcttgaca aacggttttc ggcaactgta aatgcgctat gatgctttta ttcttttcag
     1021 cgtgtaactt tgttaaatat aattgacttt ccgactatcg acgttcgttt catctccgtg
     1081 ttattgttta atggccattt tctgtggtaa actgtcgcca ttttgttttc agtcgtccgt
     1141 ttgttccgcc cttactcaca gctgtgtcag cattagtgat gactgttctc acctggcctg
     1201 tttgctttca ttattgatga agagctttct agagaattct gaagctcgtc tttgtcctga
     1261 attcatctgt attcatttaa aaaaaaaaat taaccttaat ttttttttct tctcagcgta
     1321 aagccaccat ggagatctcg agactagtaa ttaagtctca gccaccgtta actgaacatg
     1381 tcaaaacctg tggagactgt tgagatttga tgttctgaaa agataaagcc tataaataaa
     1441 atgttgccca aatttcctgc ctgatgtttt tctttgtctt tgctacatgg ctttgctgct
     1501 cggatcggct cactctgtgt atgccacgtt cactttgtac tctccttctc acggtaggtt
     1561 tattattttt agatgtgcag ttagtttctg tgaaataaca caccacacac tgatattgtc
     1621 tgtgcattga cttggtgagt gcacattgtt tttgatcttg acatatttat atttgattga
     1681 tcaggtgaac tgtgtgaatc taaagtgctc catacagatg ttctgcattg aaaatattct
     1741 cattttatta gtggaagtga gtgtatgcca gcggccgcgg taccctgcag tctagacatt
     1801 tcagttgacg aagacaaaca aagttctgtt gtgactatgg gggggggggg cgcctgggga
     1861 tggtctcgcc cggggagtaa ttcagggtag aaccgccact gcctttagcg acattcactg
     1921 gccgtcgttt tacaacgtcg tgactgggaa aaccctggcg ttacccaact taatcgcctt
     1981 gcagcacatc cccctttcgc cagctggcgt aatagcgaag aggcccgcac cgatcgccct
     2041 tcccaacagt tgcgcagcct gaatggcgaa tggcgcctga tgcggtattt tctccttacg
     2101 catctgtgcg gtatttcaca ccgcatatgg tgcactctca gtacaatctg ctctgatgcc
     2161 gcatagttaa gccagccccg acacccgcca acacccgctg acgcgccctg acgggcttgt
     2221 ctgctcccgg catccgctta cagacaagct gtgaccgtct ccgggagctg catgtgtcag
     2281 aggttttcac cgtcatcacc gaaacgcgcg agacgaaagg gcctcgtgat acgcctattt
     2341 ttataggtta atgtcatgat aataatggtt tcttagacgt caggtggcac ttttcgggga
     2401 aatgtgcgcg gaacccctat ttgtttattt ttctaaatac attcaaatat gtatccgctc
     2461 atgagacaat aaccctgata aatgcttcaa taatattgaa aaaggaagag tatgagtatt
     2521 caacatttcc gtgtcgccct tattcccttt tttgcggcat tttgccttcc tgtttttgct
     2581 cacccagaaa cgctggtgaa agtaaaagat gctgaagatc agttgggtgc acgagtgggt
     2641 tacatcgaac tggatctcaa cagcggtaag atccttgaga gttttcgccc cgaagaacgt
     2701 tttccaatga tgagcacttt taaagttctg ctatgtggcg cggtattatc ccgtattgac
     2761 gccgggcaag agcaactcgg tcgccgcata cactattctc agaatgactt ggttgagtac
     2821 tcaccagtca cagaaaagca tcttacggat ggcatgacag taagagaatt atgcagtgct
     2881 gccataacca tgagtgataa cactgcggcc aacttacttc tgacaacgat cggaggaccg
     2941 aaggagctaa ccgctttttt gcacaacatg ggggatcatg taactcgcct tgatcgttgg
     3001 gaaccggagc tgaatgaagc cataccaaac gacgagcgtg acaccacgat gcctgtagca
     3061 atggcaacaa cgttgcgcaa actattaact ggcgaactac ttactctagc ttcccggcaa
     3121 caattaatag actggatgga ggcggataaa gttgcaggac cacttctgcg ctcggccctt
     3181 ccggctggct ggtttattgc tgataaatct ggagccggtg agcgtgggtc tcgcggtatc
     3241 attgcagcac tggggccaga tggtaagccc tcccgtatcg tagttatcta cacgacgggg
     3301 agtcaggcaa ctatggatga acgaaataga cagatcgctg agataggtgc ctcactgatt
     3361 aagcattggt aactgtcaga ccaagtttac tcatatatac tttagattga tttaaaactt
     3421 catttttaat ttaaaaggat ctaggtgaag atcctttttg ataatctcat gaccaaaatc
     3481 ccttaacgtg agttttcgtt ccactgagcg tcagaccccg tagaaaagat caaaggatct
     3541 tcttgagatc ctttttttct gcgcgtaatc tgctgcttgc aaacaaaaaa accaccgcta
     3601 ccagcggtgg tttgtttgcc ggatcaagag ctaccaactc tttttccgaa ggtaactggc
     3661 ttcagcagag cgcagatacc aaatactgtt cttctagtgt agccgtagtt aggccaccac
     3721 ttcaagaact ctgtagcacc gcctacatac ctcgctctgc taatcctgtt accagtggct
     3781 gctgccagtg gcgataagtc gtgtcttacc gggttggact caagacgata gttaccggat
     3841 aaggcgcagc ggtcgggctg aacggggggt tcgtgcacac agcccagctt ggagcgaacg
     3901 acctacaccg aactgagata cctacagcgt gagctatgag aaagcgccac gcttcccgaa
     3961 gggagaaagg cggacaggta tccggtaagc ggcagggtcg gaacaggaga gcgcacgagg
     4021 gagcttccag ggggaaacgc ctggtatctt tatagtcctg tcgggtttcg ccacctctga
     4081 cttgagcgtc gatttttgtg atgctcgtca ggggggcgga gcctatggaa aaacgccagc
     4141 aacgcggcct ttttacggtt cctggccttt tgctggcctt ttgctcacat gttctttcct
     4201 gcgttatccc ctgattctgt ggataaccgt attaccgcct ttgagtgagc tgataccgct
     4261 cgccgcagcc gaacgaccga gcgcagcgag tcagtgagcg aggaagcgga aga
//
