LOCUS       sequence-101857-         814 bp    DNA     linear   UNA 15-DEC-2025
DEFINITION  Expresses human Cezanne (OTU domain) in E. coli..
ACCESSION   .
VERSION     .
KEYWORDS    .
SOURCE      natural DNA sequence
  ORGANISM  unspecified
REFERENCE   1  (bases 1 to 814)
  AUTHORS   Mevissen TE, Hospenthal MK, Geurink PP, Elliott PR, Akutsu M, 
            Arnaudo N, Ekkebus R, Kulathu Y, Wauer T, El Oualid F, Freund SM, 
            Ovaa H, Komander D
  TITLE     OTU Deubiquitinases Reveal Mechanisms of Linkage Specificity and 
            Enable Ubiquitin Chain Restriction Analysis.
  JOURNAL   Cell. 2013 Jul 3;154(1):169-84. doi: 10.1016/j.cell.2013.05.046.
  PUBMED    23827681
REFERENCE   2  (bases 1 to 814)
  AUTHORS   .
  TITLE     Direct Submission
  JOURNAL   Exported Dec 15, 2025 from SnapGene Server 8.0.1
            https://www.snapgene.com
COMMENT     SGRef: number: 1; type: "Journal Article"; doi: 
            "10.1016/j.cell.2013.05"; journalName: "Cell"; date: "2013-07-3- 3";
            volume: "154"; issue: "1"; pages: "169-84"
COMMENT     Sequence Label: pOPINK-Cezanne (OTU, aa 53-446)
FEATURES             Location/Qualifiers
     source          1..814
                     /mol_type="genomic DNA"
                     /organism="unspecified"
     CDS             779..796
                     /codon_start=1
                     /product="6xHis affinity tag"
                     /label=6xHis
                     /translation="HHHHHH"
ORIGIN
        1 tcctgcatgc agcctccctt ggaatgtggg gtttccatga tcgggacttg atgctgcgga
       61 aagctttgta tgcactgatg gagaagggag ttgagaagga agcgttgaaa aggcgctgga
      121 ggtggcagca gacacagcag aataaagagt cagggctggt atacacagaa gatgaatggc
      181 agaaggagtg gaatgaactg atcaagcttg cctcaagtga accccgaatg catctaggta
      241 ccaatggagc caactgtggt ggggtggaga gttctgagga gcctgtatat gagagccttg
      301 aagagtttca cgtctttgtc cttgctcatg tgcttaggag gcccatagtc gtcgtggcag
      361 acaccatgct gagggactcc ggaggggaag catttgcccc tattcccttt ggaggaatct
      421 atctgccttt ggaggtccca gccagccagt gtcaccgctc ccctctggtg ctcgcctatg
      481 atcaggccca cttttctgca ctcgtgtcca tggagcagaa ggagaatacc aaggaacaag
      541 ctgtgatccc acttacagat tcagagtata agctgctgcc cttgcacttt gctgtggacc
      601 ctggaaaggg ctgggagtgg ggcaaagatg atagtgacaa tgtccgattg gccagtgtaa
      661 ttctgtccct agaggtcaaa ttgcatctgc tgcatagcta catgaatgtg aagtggatcc
      721 cactgtcctc tgatgcacag gctcctctgg cccagtaaag ctttctagac catttaaaca
      781 ccaccaccac caccactgag atccggctgc taac
//
