LOCUS       sequence-101858-         658 bp    DNA     linear   UNA 15-DEC-2025
DEFINITION  Expresses human OTULIN (full-length) in E. coli..
ACCESSION   .
VERSION     .
KEYWORDS    .
SOURCE      natural DNA sequence
  ORGANISM  unspecified
REFERENCE   1  (bases 1 to 658)
  AUTHORS   Keusekotten K, Elliott PR, Glockner L, Fiil BK, Damgaard RB, Kulathu
            Y, Wauer T, Hospenthal MK, Gyrd-Hansen M, Krappmann D, Hofmann K, 
            Komander D
  TITLE     OTULIN antagonizes LUBAC signaling by specifically hydrolyzing 
            Met1-linked polyubiquitin.
  JOURNAL   Cell. 2013 Jun 6;153(6):1312-26. doi: 10.1016/j.cell.2013.05.014.
  PUBMED    23746843
REFERENCE   2  (bases 1 to 658)
  AUTHORS   .
  TITLE     Direct Submission
  JOURNAL   Exported Dec 15, 2025 from SnapGene Server 8.0.1
            https://www.snapgene.com
COMMENT     SGRef: number: 1; type: "Journal Article"; doi: 
            "10.1016/j.cell.2013.05"; journalName: "Cell"; date: "2013-06-6- 6";
            volume: "153"; issue: "6"; pages: "1312-26"
COMMENT     Sequence Label: pOPINB-OTULIN (full-length, aa 1-352)
FEATURES             Location/Qualifiers
     source          1..658
                     /mol_type="genomic DNA"
                     /organism="unspecified"
     CDS             623..640
                     /codon_start=1
                     /product="6xHis affinity tag"
                     /label=6xHis
                     /translation="HHHHHH"
ORIGIN
        1 agctcatgct gttaccagaa aaactcataa gcaaatacaa ctggatcaag caatggaaac
       61 ttggactgaa atttgatggg aagaatgagg acctggttga taaaattaaa gagtccctta
      121 ctctgctgag gaagaagtgg gcaggcttgg ctgaaatgag aactgctgaa gcaagacaga
      181 tagcttgtga tgaactattc acaaatgagg cggaggaata tagcctctat gaagctgtaa
      241 aatttctaat gctaaacaga gccattgaac tatataatga taaagagaaa ggaaaggaag
      301 taccattttt ctctgtgctt ctgtttgctc gggacacatc aaatgaccca ggacagcttc
      361 tgaggaacca cctcaaccag gtgggacaca ctggtggtct tgaacaggtt gaaatgttcc
      421 ttcttgccta tgctgtgcgc cacaccatcc aggtgtaccg gctctccaag tacaacacgg
      481 aagaattcat cacagtctac cccaccgacc cacccaagga ctggccagtg gtaacgctca
      541 ttgctgagga cgatcggcac tataacatcc ccgtcagagt gtgtgaggag accagtctat
      601 aaagctttct agaccattta aacaccacca ccaccaccac tgagatccgg ctgctaac
//
