LOCUS       sequence-101862-         787 bp    DNA     linear   UNA 15-DEC-2025
DEFINITION  Expresses human OTUB1 (full-length) in E. coli..
ACCESSION   .
VERSION     .
KEYWORDS    .
SOURCE      natural DNA sequence
  ORGANISM  unspecified
REFERENCE   1  (bases 1 to 787)
  AUTHORS   Mevissen TE, Hospenthal MK, Geurink PP, Elliott PR, Akutsu M, 
            Arnaudo N, Ekkebus R, Kulathu Y, Wauer T, El Oualid F, Freund SM, 
            Ovaa H, Komander D
  TITLE     OTU Deubiquitinases Reveal Mechanisms of Linkage Specificity and 
            Enable Ubiquitin Chain Restriction Analysis.
  JOURNAL   Cell. 2013 Jul 3;154(1):169-84. doi: 10.1016/j.cell.2013.05.046.
  PUBMED    23827681
REFERENCE   2  (bases 1 to 787)
  AUTHORS   .
  TITLE     Direct Submission
  JOURNAL   Exported Dec 15, 2025 from SnapGene Server 8.0.1
            https://www.snapgene.com
COMMENT     SGRef: number: 1; type: "Journal Article"; doi: 
            "10.1016/j.cell.2013.05"; journalName: "Cell"; date: "2013-07-3- 3";
            volume: "154"; issue: "1"; pages: "169-84"
COMMENT     Sequence Label: pOPINK-OTUB1 (full-length, aa 1-271)
FEATURES             Location/Qualifiers
     source          1..787
                     /mol_type="genomic DNA"
                     /organism="unspecified"
     CDS             752..769
                     /codon_start=1
                     /product="6xHis affinity tag"
                     /label=6xHis
                     /translation="HHHHHH"
ORIGIN
        1 ccatcatggc tcagcaggac cgaattcagc aagagattgc tgtgcagaac cctctggtgt
       61 cagagcggct ggagctctcg gtcctataca aggagtatgc tgaagatgac aacatctatc
      121 aacagaagat caaggacctc cacaaaaagt actcgtacat ccgcaagacc aggcctgacg
      181 gcaactgttt ctatcgggct ttcggattct cccacttgga ggcactgctg gatgacagca
      241 aggagttgca gcggttcaag gctgtgtctg ccaagagcaa ggaagacctg gtgtcccagg
      301 gcttcactga attcacaatt gaggatttcc acaacacgtt catggacctg attgagcagg
      361 tggagaagca gacctctgtc gccgacctgc tggcctcctt caatgaccag agcacctccg
      421 actaccttgt ggtctacctg cggctgctca cctcgggcta cctgcagcgc gagagcaagt
      481 tcttcgagca cttcatcgag ggtggacgga ctgtcaagga gttctgccag caggaggtgg
      541 agcccatgtg caaggagagc gaccacatcc acatcattgc gctggcccag gccctcagcg
      601 tgtccatcca ggtggagtac atggaccgcg gcgagggcgg caccaccaat ccgcacatct
      661 tccctgaggg ctccgagccc aaggtctacc ttctctaccg gcctggacac tacgatatcc
      721 tctacaaata aagctttcta gaccatttaa acaccaccac caccaccact gagatccggc
      781 tgctaac
//
