LOCUS       sequence-101879-         892 bp    DNA     linear   UNA 15-DEC-2025
DEFINITION  Expresses human OTUD1 (OTU+UIM domains) in E. coli..
ACCESSION   .
VERSION     .
KEYWORDS    .
SOURCE      natural DNA sequence
  ORGANISM  unspecified
REFERENCE   1  (bases 1 to 892)
  AUTHORS   Mevissen TE, Hospenthal MK, Geurink PP, Elliott PR, Akutsu M, 
            Arnaudo N, Ekkebus R, Kulathu Y, Wauer T, El Oualid F, Freund SM, 
            Ovaa H, Komander D
  TITLE     OTU Deubiquitinases Reveal Mechanisms of Linkage Specificity and 
            Enable Ubiquitin Chain Restriction Analysis.
  JOURNAL   Cell. 2013 Jul 3;154(1):169-84. doi: 10.1016/j.cell.2013.05.046.
  PUBMED    23827681
REFERENCE   2  (bases 1 to 892)
  AUTHORS   .
  TITLE     Direct Submission
  JOURNAL   Exported Dec 15, 2025 from SnapGene Server 8.0.1
            https://www.snapgene.com
COMMENT     SGRef: number: 1; type: "Journal Article"; doi: 
            "10.1016/j.cell.2013.05"; journalName: "Cell"; date: "2013-07-3- 3";
            volume: "154"; issue: "1"; pages: "169-84"
COMMENT     Sequence Label: pOPINK-OTUD1 (OTU+UIM, aa 287-481)
FEATURES             Location/Qualifiers
     source          1..892
                     /mol_type="genomic DNA"
                     /organism="unspecified"
     primer_bind     164..186
                     /label=pGEX 5'
                     /note="pGEX vectors, Glutathione-S-transferase, forward 
                     primer"
     CDS             225..248
                     /codon_start=1
                     /product="recognition and cleavage site for human 
                     rhinovirus 3C and PreScission proteases"
                     /label=HRV 3C site
                     /translation="LEVLFQGP"
     CDS             857..874
                     /codon_start=1
                     /product="6xHis affinity tag"
                     /label=6xHis
                     /translation="HHHHHH"
ORIGIN
        1 atcctgactt catgttgtat gacgctcttg atgttgtttt atacatggac ccaatgtgcc
       61 tggatgcgtt cccaaaatta gtttgtttta aaaaacgtat tgaagctatc ccacaaattg
      121 ataagtactt gaaatccagc aagtatatag catggccttt gcagggctgg caagccacgt
      181 ttggtggtgg cgaccatcct ccaaaatcgg atctgagcag cggtctggaa gttctgtttc
      241 agggcccgga cgagaagctg gccctatacc tggccgaggt ggagaagcag gacaagtatc
      301 tgcggcagag gaataagtac cgattccaca tcattccaga cggcaactgc ctctaccgag
      361 ctgtcagcaa gacggtgtat ggggaccaga gcctgcaccg ggagttgagg gagcagacgg
      421 tgcactacat cgccgaccat ctcgaccact tcagccccct gattgagggc gacgtggggg
      481 agtttatcat cgctgctgcc caagacgggg catgggccgg gtacccggag ttgctggcca
      541 tggggcagat gctgaatgtg aatatccatt taactactgg agggaggctg gagagtccca
      601 cggtgtctac catgattcat tatttgggcc cagaggattc cctgaggcct agtatttggc
      661 tcagttggct cagtaacgga cactatgatg ctgtatttga tcactcctat cctaacccag
      721 agtacgacaa ctggtgcaaa caaactcaag tgcaaaggaa acgcgacgaa gaacttgcca
      781 aatctatggc catatccttg tctaaaatgt atattgaaca aaatgcatgc tcttaaagct
      841 ttctagacca tttaaacacc accaccacca ccactgagat ccggctgcta ac
//
