LOCUS       sequence-101881-         861 bp    DNA     linear   UNA 15-DEC-2025
DEFINITION  Localization: Nucleus/Histones, Excitation: 587, Emission: 610.
ACCESSION   .
VERSION     .
KEYWORDS    .
SOURCE      natural DNA sequence
  ORGANISM  unspecified
REFERENCE   1  (bases 1 to 861)
  TITLE     Davidson lab - mCherry constructs
REFERENCE   2  (bases 1 to 861)
  AUTHORS   .
  TITLE     Direct Submission
  JOURNAL   Exported Dec 15, 2025 from SnapGene Server 8.0.1
            https://www.snapgene.com
COMMENT     SGRef: number: 1; type: "Journal Article"
COMMENT     Sequence Label: mCherry-H2B-C-10
FEATURES             Location/Qualifiers
     source          1..861
                     /mol_type="genomic DNA"
                     /organism="unspecified"
     CDS             282..659
                     /codon_start=1
                     /gene="HIST1H2BJ"
                     /product="human histone H2B"
                     /label=H2B
                     /translation="PEPAKSAPAPKKGSKKAVTKAQKKGGKKRKRSRKESYSIYVYKVL
                     KQVHPDTGISSKAMGIMNSFVNDIFERIAGEASRLAHYNKRSTITSREIQTAVRLLLPG
                     ELAKHAVSEGTKAITKYTSAK"
     primer_bind     complement(826..845)
                     /label=SV40pA-R
                     /note="SV40 polyA, reverse primer"
ORIGIN
        1 agcggatgta ccccgaggac ggcgccctga agggcgagat caagcagagg ctgaagctga
       61 aggacggcgg ccactacgac gctgaggtca agaccaccta caaggccaag aagcccgtgc
      121 agctgcccgg cgcctacaac gtcaacatca agttggacat cacctcccac aacgaggact
      181 acaccatcgt ggaacagtac gaacgcgccg agggccgcca ctccaccggc ggcatggacg
      241 agctgtacaa gtccggactc agatctggag gctccggagg cccagagcca gcgaagtctg
      301 ctcccgcccc gaaaaagggc tccaagaagg cggtgactaa ggcgcagaag aaaggcggca
      361 agaagcgcaa gcgcagccgc aaggagagct attccatcta tgtgtacaag gttctgaagc
      421 aggtccaccc tgacaccggc atttcgtcca aggccatggg catcatgaat tcgtttgtga
      481 acgacatttt cgagcgcatc gcaggtgagg cttcccgcct ggcgcattac aacaagcgct
      541 cgaccatcac ctccagggag atccagacgg ccgtgcgcct gctgctgcct ggggagttgg
      601 ccaagcacgc cgtgtccgag ggtactaagg ccatcaccaa gtacaccagc gctaagtaag
      661 gatccaccgg atctagataa ctgatcataa tcagccatac cacatttgta gaggttttac
      721 ttgctttaaa aaacctccca cacctccccc tgaacctgaa acataaaatg aatgcaattg
      781 ttgttgttaa cttgtttatt gcagcttata atggttacaa ataaagcaat agcatcacaa
      841 atttcacaaa taaagcattt t
//
