LOCUS       sequence-101893-         695 bp    DNA     linear   UNA 15-DEC-2025
DEFINITION  Expresses human OTUD6A (OTU domain) in E. coli..
ACCESSION   .
VERSION     .
KEYWORDS    .
SOURCE      natural DNA sequence
  ORGANISM  unspecified
REFERENCE   1  (bases 1 to 695)
  AUTHORS   Mevissen TE, Hospenthal MK, Geurink PP, Elliott PR, Akutsu M, 
            Arnaudo N, Ekkebus R, Kulathu Y, Wauer T, El Oualid F, Freund SM, 
            Ovaa H, Komander D
  TITLE     OTU Deubiquitinases Reveal Mechanisms of Linkage Specificity and 
            Enable Ubiquitin Chain Restriction Analysis.
  JOURNAL   Cell. 2013 Jul 3;154(1):169-84. doi: 10.1016/j.cell.2013.05.046.
  PUBMED    23827681
REFERENCE   2  (bases 1 to 695)
  AUTHORS   .
  TITLE     Direct Submission
  JOURNAL   Exported Dec 15, 2025 from SnapGene Server 8.0.1
            https://www.snapgene.com
COMMENT     SGRef: number: 1; type: "Journal Article"; doi: 
            "10.1016/j.cell.2013.05"; journalName: "Cell"; date: "2013-07-3- 3";
            volume: "154"; issue: "1"; pages: "169-84"
COMMENT     Sequence Label: pOPINK-OTUD6A (OTU, aa 128-288)
FEATURES             Location/Qualifiers
     source          1..695
                     /mol_type="genomic DNA"
                     /organism="unspecified"
     primer_bind     69..91
                     /label=pGEX 5'
                     /note="pGEX vectors, Glutathione-S-transferase, forward 
                     primer"
     CDS             130..153
                     /codon_start=1
                     /product="recognition and cleavage site for human 
                     rhinovirus 3C and PreScission proteases"
                     /label=HRV 3C site
                     /translation="LEVLFQGP"
     CDS             660..677
                     /codon_start=1
                     /product="6xHis affinity tag"
                     /label=6xHis
                     /translation="HHHHHH"
ORIGIN
        1 cgtattgaag ctatcccaca aattgataag tacttgaaat ccagcaagta tatagcatgg
       61 cctttgcagg gctggcaagc cacgtttggt ggtggcgacc atcctccaaa atcggatctg
      121 agcagcggtc tggaagttct gtttcagggc ccggaggagg agaagctcgc cgccatcctg
      181 ggagccaggg gtctggagat gaaagcgatc ccggccgacg gccactgcat gtaccgcgcc
      241 atccaagacc agctggtgtt cagcgtgtct gtggagatgc tgcgctgccg caccgccagc
      301 tacatgaaga agcacgtcga cgagttcctg cccttcttca gcaaccccga gaccagcgac
      361 tccttcggct acgacgactt catgatctac tgcgacaaca tcgtgcgcac cacggcatgg
      421 ggaggccagc tggagctgag ggccctgtcg cacgtcctga agacccccat cgaggtgatc
      481 caggccgact cgcccacctt gatcatcggg gaggagtacg tcaagaagcc gatcatcctg
      541 gtctacctgc gctatgccta cagcctcggc gagcactaca actccgtgac accgctcgag
      601 gccggcgccg ccgggggcgt gctcccgcgt ctcctgtaaa gctttctaga ccatttaaac
      661 accaccacca ccaccactga gatccggctg ctaac
//
