LOCUS       sequence-101895-         830 bp    DNA     linear   UNA 15-DEC-2025
DEFINITION  Expresses human OTUD3 (OTU+UBA domains) in E. coli..
ACCESSION   .
VERSION     .
KEYWORDS    .
SOURCE      natural DNA sequence
  ORGANISM  unspecified
REFERENCE   1  (bases 1 to 830)
  AUTHORS   Mevissen TE, Hospenthal MK, Geurink PP, Elliott PR, Akutsu M, 
            Arnaudo N, Ekkebus R, Kulathu Y, Wauer T, El Oualid F, Freund SM, 
            Ovaa H, Komander D
  TITLE     OTU Deubiquitinases Reveal Mechanisms of Linkage Specificity and 
            Enable Ubiquitin Chain Restriction Analysis.
  JOURNAL   Cell. 2013 Jul 3;154(1):169-84. doi: 10.1016/j.cell.2013.05.046.
  PUBMED    23827681
REFERENCE   2  (bases 1 to 830)
  AUTHORS   .
  TITLE     Direct Submission
  JOURNAL   Exported Dec 15, 2025 from SnapGene Server 8.0.1
            https://www.snapgene.com
COMMENT     SGRef: number: 1; type: "Journal Article"; doi: 
            "10.1016/j.cell.2013.05"; journalName: "Cell"; date: "2013-07-3- 3";
            volume: "154"; issue: "1"; pages: "169-84"
COMMENT     Sequence Label: pOPINK-OTUD3 (OTU+UBA, aa 52-275)
FEATURES             Location/Qualifiers
     source          1..830
                     /mol_type="genomic DNA"
                     /organism="unspecified"
     primer_bind     16..38
                     /label=pGEX 5'
                     /note="pGEX vectors, Glutathione-S-transferase, forward 
                     primer"
     CDS             77..100
                     /codon_start=1
                     /product="recognition and cleavage site for human 
                     rhinovirus 3C and PreScission proteases"
                     /label=HRV 3C site
                     /translation="LEVLFQGP"
     CDS             796..813
                     /codon_start=1
                     /product="6xHis affinity tag"
                     /label=6xHis
                     /translation="HHHHHH"
ORIGIN
        1 agcatggcct ttgcagggct ggcaagccac gtttggtggt ggcgaccatc ctccaaaatc
       61 ggatctgagc agcggtctgg aagttctgtt tcagggcccg gagttcgtca gcttcgccaa
      121 ccagctgcag gccctggggc tgaagctgcg ggaggtgccg ggggacggca attgcttgtt
      181 cagagctctt ggtgatcaat tggagggaca ctcacgaaat catctcaagc acagacagga
      241 gacagtggac tacatgataa agcagcggga agattttgaa ccctttgtag aagatgacat
      301 tccttttgag aagcatgtgg ccagtttggc aaagcctggt acttttgctg gcaatgatgc
      361 aattgtagcc tttgcaagaa atcatcagtt gaatgtagtg attcatcaac ttaatgcccc
      421 tttgtggcag attcgtggta cagagaaaag cagcgtgagg gagttacaca tcgcatatcg
      481 gtatggagag cactacgaca gtgttcggag gatcaatgac aactcagagg cacctgcaca
      541 tctccagacg gattttcaga tgcttcatca agatgaatca aataaaagag aaaagatcaa
      601 gacaaaggga atggactctg aagacgacct gagagatgaa gtagaggatg ctgtccagaa
      661 agtttgtaat gcaactggat gttcagattt taatttaata gtccagaacc tggaagctga
      721 aaattataat attgaatctg caataattgc cgtgcttcgg atgaaccaag ggtaaagctt
      781 tctagaccat ttaaacacca ccaccaccac cactgagatc cggctgctaa
//
