LOCUS       sequence-101897-         881 bp    DNA     linear   UNA 15-DEC-2025
DEFINITION  Expresses human OTUD6B (OTU domain) in E. coli..
ACCESSION   .
VERSION     .
KEYWORDS    .
SOURCE      natural DNA sequence
  ORGANISM  unspecified
REFERENCE   1  (bases 1 to 881)
  AUTHORS   Mevissen TE, Hospenthal MK, Geurink PP, Elliott PR, Akutsu M, 
            Arnaudo N, Ekkebus R, Kulathu Y, Wauer T, El Oualid F, Freund SM, 
            Ovaa H, Komander D
  TITLE     OTU Deubiquitinases Reveal Mechanisms of Linkage Specificity and 
            Enable Ubiquitin Chain Restriction Analysis.
  JOURNAL   Cell. 2013 Jul 3;154(1):169-84. doi: 10.1016/j.cell.2013.05.046.
  PUBMED    23827681
REFERENCE   2  (bases 1 to 881)
  AUTHORS   .
  TITLE     Direct Submission
  JOURNAL   Exported Dec 15, 2025 from SnapGene Server 8.0.1
            https://www.snapgene.com
COMMENT     SGRef: number: 1; type: "Journal Article"; doi: 
            "10.1016/j.cell.2013.05"; journalName: "Cell"; date: "2013-07-3- 3";
            volume: "154"; issue: "1"; pages: "169-84"
COMMENT     Sequence Label: pOPINK-OTUD6B (OTU, aa 133-293)
FEATURES             Location/Qualifiers
     source          1..881
                     /mol_type="genomic DNA"
                     /organism="unspecified"
     primer_bind     255..277
                     /label=pGEX 5'
                     /note="pGEX vectors, Glutathione-S-transferase, forward 
                     primer"
     CDS             316..339
                     /codon_start=1
                     /product="recognition and cleavage site for human 
                     rhinovirus 3C and PreScission proteases"
                     /label=HRV 3C site
                     /translation="LEVLFQGP"
     CDS             846..863
                     /codon_start=1
                     /product="6xHis affinity tag"
                     /label=6xHis
                     /translation="HHHHHH"
ORIGIN
        1 gttgattttc ttagcaagct acctgaaatg ctgaaaatgt tcgaagatcg tttatgtcat
       61 aaaacatatt taaatggtga tcatgtaacc catcctgact tcatgttgta tgacgctctt
      121 gatgttgttt tatacatgga cccaatgtgc ctggatgcgt tcccaaaatt agtttgtttt
      181 aaaaaacgta ttgaagctat cccacaaatt gataagtact tgaaatccag caagtatata
      241 gcatggcctt tgcagggctg gcaagccacg tttggtggtg gcgaccatcc tccaaaatcg
      301 gatctgagca gcggtctgga agttctgttt cagggcccga tggaaagtga gaaacttgct
      361 caaatattgg cagctagaca gttagaaatt aaacagattc catctgatgg ccactgtatg
      421 tataaagcca ttgaagatca actgaaagaa aaggattgtg ctctgactgt ggttgccttg
      481 agaagtcaga ccgctgagta tatgcaaagc catgtggaag actttctgcc atttttaaca
      541 aaccctaata caggagatat gtatactcca gaagaatttc agaagtactg tgaagatatt
      601 gtaaacacag ctgcatgggg aggtcagctt gagctaagag ctctgtctca cattttacaa
      661 acaccaatag agataataca ggcagattct cctcccatta tagttggtga agaatattca
      721 aaaaaaccac taatacttgt atatatgaga catgcatatg gcttaggaga acattataat
      781 tcggttacac ggttggtaaa catagttact gaaaattgca gctaaagctt tctagaccat
      841 ttaaacacca ccaccaccac cactgagatc cggctgctaa c
//
