LOCUS       sequence-101899-         849 bp    DNA     linear   UNA 15-DEC-2025
DEFINITION  Expresses human OTUD4 (OTU domain) in E. coli..
ACCESSION   .
VERSION     .
KEYWORDS    .
SOURCE      natural DNA sequence
  ORGANISM  unspecified
REFERENCE   1  (bases 1 to 849)
  AUTHORS   Mevissen TE, Hospenthal MK, Geurink PP, Elliott PR, Akutsu M, 
            Arnaudo N, Ekkebus R, Kulathu Y, Wauer T, El Oualid F, Freund SM, 
            Ovaa H, Komander D
  TITLE     OTU Deubiquitinases Reveal Mechanisms of Linkage Specificity and 
            Enable Ubiquitin Chain Restriction Analysis.
  JOURNAL   Cell. 2013 Jul 3;154(1):169-84. doi: 10.1016/j.cell.2013.05.046.
  PUBMED    23827681
REFERENCE   2  (bases 1 to 849)
  AUTHORS   .
  TITLE     Direct Submission
  JOURNAL   Exported Dec 15, 2025 from SnapGene Server 8.0.1
            https://www.snapgene.com
COMMENT     SGRef: number: 1; type: "Journal Article"; doi: 
            "10.1016/j.cell.2013.05"; journalName: "Cell"; date: "2013-07-3- 3";
            volume: "154"; issue: "1"; pages: "169-84"
COMMENT     Sequence Label: pOPINK-OTUD4 (OTU, aa 22-196)
FEATURES             Location/Qualifiers
     source          1..849
                     /mol_type="genomic DNA"
                     /organism="unspecified"
     primer_bind     181..203
                     /label=pGEX 5'
                     /note="pGEX vectors, Glutathione-S-transferase, forward 
                     primer"
     CDS             242..265
                     /codon_start=1
                     /product="recognition and cleavage site for human 
                     rhinovirus 3C and PreScission proteases"
                     /label=HRV 3C site
                     /translation="LEVLFQGP"
     CDS             814..831
                     /codon_start=1
                     /product="6xHis affinity tag"
                     /label=6xHis
                     /translation="HHHHHH"
ORIGIN
        1 tggtgatcat gtaacccatc ctgacttcat gttgtatgac gctcttgatg ttgttttata
       61 catggaccca atgtgcctgg atgcgttccc aaaattagtt tgttttaaaa aacgtattga
      121 agctatccca caaattgata agtacttgaa atccagcaag tatatagcat ggcctttgca
      181 gggctggcaa gccacgtttg gtggtggcga ccatcctcca aaatcggatc tgagcagcgg
      241 tctggaagtt ctgtttcagg gcccggcgac gcccatggac gcctatctgc ggaaactggg
      301 cttgtatcgg aaactggtcg ccaaggacgg gtcgtgcctg ttccgggccg tggcggagca
      361 ggtattgcac tctcagtctc gccatgttga agtcagaatg gcctgtattc actatcttcg
      421 agagaacaga gagaaatttg aagcgtttat agaaggatca tttgaagaat atttaaagcg
      481 tttggaaaat ccacaggaat gggtaggaca agtggaaata agtgcccttt ctcttatgta
      541 caggaaagat tttataattt atcgggaacc aaatgtttct ccttcacaag taacagaaaa
      601 taattttcct gaaaaggtgt tactgtgttt ttcaaatgga aatcattatg atattgtgta
      661 tcccataaag tataaagaaa gctctgctat gtgtcagtct ctcctttatg aattgctgta
      721 tgagaaggta tttaaaactg atgttagtaa aattgtgatg gaactagaca cgttggaagt
      781 agctgatgaa taaagctttc tagaccattt aaacaccacc accaccacca ctgagatccg
      841 gctgctaac
//
