LOCUS       sequence-101901-         824 bp    DNA     linear   UNA 15-DEC-2025
DEFINITION  Localization: Nucleus/Centromeres, Excitation: 587, Emission: 610.
ACCESSION   .
VERSION     .
KEYWORDS    .
SOURCE      natural DNA sequence
  ORGANISM  unspecified
REFERENCE   1  (bases 1 to 824)
  TITLE     Davidson lab - mCherry constructs
REFERENCE   2  (bases 1 to 824)
  AUTHORS   .
  TITLE     Direct Submission
  JOURNAL   Exported Dec 15, 2025 from SnapGene Server 8.0.1
            https://www.snapgene.com
COMMENT     SGRef: number: 1; type: "Journal Article"
COMMENT     Sequence Label: mCherry-CENPB-N-22
FEATURES             Location/Qualifiers
     source          1..824
                     /mol_type="genomic DNA"
                     /organism="unspecified"
     regulatory      542..551
                     /label=Kozak sequence
                     /note="vertebrate consensus sequence for strong initiation 
                     of translation (Kozak, 1987)"
                     /regulatory_class="other"
     promoter        complement(578..781)
                     /label=CMV promoter
                     /note="human cytomegalovirus (CMV) immediate early 
                     promoter"
     primer_bind     complement(607..627)
                     /label=CMV-F
                     /note="Human CMV immediate early promoter, forward primer"
ORIGIN
        1 ggtaccgtcg actgcagaat tcgaagcttg agctcgagag tgctcccgcc actgccctcc
       61 gagggcaccg cggccggact ggcaggcgcc gccggggtgc ggggggcagc gtttcgcgcg
      121 cgggcgcggg ccacgccgct gcaggacacc acgccgtggc gccggcggaa gcggtccagc
      181 cagccgttgg aggcggtgaa gtcgtccatg cccagctcct cggctatgcg cagcgccttc
      241 tccttgagga tgatgccctt gaccggcagg ccggcggcgc ggatctgctg gaaccaggcg
      301 atgagcaagc cctcgagctt gtcgtaggga gacagcttgt tggtcttgcg gcaggtggag
      361 gccaccccgt acttgcgctc cgacgccagg atggcgcgct tgttcttcag gatcgtgctc
      421 agcgtggacg gcgggatgtt gaagcgccgc gcgatctcgc ccttgcgtag gtccgggttc
      481 tcctccacct cctggatgat ccgcgacttc tcccggaacg tcagctgccg ccgcttgggg
      541 cccatggtgg ctagcggatc tgacggttca ctaaaccagc tctgcttata tagacctccc
      601 accgtacacg cctaccgccc atttgcgtca atggggcgga gttgttacga cattttggaa
      661 agtcccgttg attttggtgc caaaacaaac tcccattgac gtcaatgggg tggagacttg
      721 gaaatccccg tgagtcaaac cgctatccac gcccattgat gtactgccaa aaccgcatca
      781 ccatggtaat agcgatgact aatacgtaga tgtactgcca agta
//
