LOCUS       sequence-101903-         890 bp    DNA     linear   UNA 15-DEC-2025
DEFINITION  Expresses human OTUD2 (OTU domain) in E. coli..
ACCESSION   .
VERSION     .
KEYWORDS    .
SOURCE      natural DNA sequence
  ORGANISM  unspecified
REFERENCE   1  (bases 1 to 890)
  AUTHORS   Mevissen TE, Hospenthal MK, Geurink PP, Elliott PR, Akutsu M, 
            Arnaudo N, Ekkebus R, Kulathu Y, Wauer T, El Oualid F, Freund SM, 
            Ovaa H, Komander D
  TITLE     OTU Deubiquitinases Reveal Mechanisms of Linkage Specificity and 
            Enable Ubiquitin Chain Restriction Analysis.
  JOURNAL   Cell. 2013 Jul 3;154(1):169-84. doi: 10.1016/j.cell.2013.05.046.
  PUBMED    23827681
REFERENCE   2  (bases 1 to 890)
  AUTHORS   .
  TITLE     Direct Submission
  JOURNAL   Exported Dec 15, 2025 from SnapGene Server 8.0.1
            https://www.snapgene.com
COMMENT     SGRef: number: 1; type: "Journal Article"; doi: 
            "10.1016/j.cell.2013.05"; journalName: "Cell"; date: "2013-07-3- 3";
            volume: "154"; issue: "1"; pages: "169-84"
COMMENT     Sequence Label: pOPINK-OTUD2 (OTU, aa 132-314)
FEATURES             Location/Qualifiers
     source          1..890
                     /mol_type="genomic DNA"
                     /organism="unspecified"
     primer_bind     198..220
                     /label=pGEX 5'
                     /note="pGEX vectors, Glutathione-S-transferase, forward 
                     primer"
     CDS             259..282
                     /codon_start=1
                     /product="recognition and cleavage site for human 
                     rhinovirus 3C and PreScission proteases"
                     /label=HRV 3C site
                     /translation="LEVLFQGP"
     CDS             855..872
                     /codon_start=1
                     /product="6xHis affinity tag"
                     /label=6xHis
                     /translation="HHHHHH"
ORIGIN
        1 cataaaacat atttaaatgg tgatcatgta acccatcctg acttcatgtt gtatgacgct
       61 cttgatgttg ttttatacat ggacccaatg tgcctggatg cgttcccaaa attagtttgt
      121 tttaaaaaac gtattgaagc tatcccacaa attgataagt acttgaaatc cagcaagtat
      181 atagcatggc ctttgcaggg ctggcaagcc acgtttggtg gtggcgacca tcctccaaaa
      241 tcggatctga gcagcggtct ggaagttctg tttcagggcc cggcatttac taaacgtggt
      301 gcttctagtt acgtcaggga aactttgcct gtgcttacca gaaccgtggt cccagcagac
      361 aactcttgcc tctttactag tgtgtactat gtcgtcgaag gaggagtctt gaatccagct
      421 tgtgcccctg agatgagacg cctcatagca caaattgtag caagcgatcc agacttctat
      481 agtgaggcaa tactgggaaa aacaaatcaa gagtactgtg actggatcaa aagggatgac
      541 acttggggag gagcaataga gatatcgatt ttgtccaagt tttaccaatg tgaaatatgt
      601 gtagtggata cacagacagt aagaattgat cgttttgggg aagatgcagg atataccaaa
      661 agggttctgc ttatttatga tggcatccac tatgatccac ttcagcgtaa cttccctgat
      721 ccagatacac ctcctctgac cattttctcc tctaatgatg atattgttct tgtacaagca
      781 ctggaattag cagatgaagc tagaagaagg agacagttta ctgatgtcaa ctaaagcttt
      841 ctagaccatt taaacaccac caccaccacc actgagatcc ggctgctaac
//
