LOCUS       sequence-101907-         985 bp    DNA     linear   UNA 15-DEC-2025
DEFINITION  Localization: Plasma Membrane, Excitation: 548, Emission: 561.
ACCESSION   .
VERSION     .
KEYWORDS    .
SOURCE      natural DNA sequence
  ORGANISM  unspecified
REFERENCE   1  (bases 1 to 985)
  TITLE     Davidson Orange Fluorescent Proteins
REFERENCE   2  (bases 1 to 985)
  AUTHORS   .
  TITLE     Direct Submission
  JOURNAL   Exported Dec 15, 2025 from SnapGene Server 8.0.1
            https://www.snapgene.com
COMMENT     SGRef: number: 1; type: "Journal Article"
COMMENT     Sequence Label: mKO-Farnesyl-5
FEATURES             Location/Qualifiers
     source          1..985
                     /mol_type="genomic DNA"
                     /organism="unspecified"
     CDS             complement(246..899)
                     /codon_start=1
                     /product="monomeric Kusabira-Orange fluorescent protein 
                     (Karasawa et al., 2004)"
                     /label=mKO
                     /translation="MVSVIKPEMKMRYYMDGSVNGHEFTIEGEGTGRPYEGHQEMTLRV
                     TMAKGGPMPFAFDLVSHVFCYGHRPFTKYPEEIPDYFKQAFPEGLSWERSLEFEDGGSA
                     SVSAHISLRGNTFYHKSKFTGVNFPADGPIMQNQSVDWEPSTEKITASDGVLKGDVTMY
                     LKLEGGGNHKCQFKTTYKAAKKILKMPGSHYISHRLVRKTEGNITELVEDAVAHS"
     promoter        complement(940..984)
                     /label=CMVd3 promoter
                     /note="truncated human cytomegalovirus (CMV) immediate 
                     early promoter that yields strongly reduced expression 
                     relative to the full-length promoter"
ORIGIN
        1 acaagttaac aacaacaatt gcattcattt tatgtttcag gttcaggggg aggtgtggga
       61 ggttttttaa agcaagtaaa acctctacaa atgtggtatg gctgattatg atcagttatc
      121 tagatccggt ggatcccggg cccgcggtac cgtcgactgc agaattctca ggagagcaca
      181 cacttgcagc tcatgcagcc ggggccactc tcatcaggag ggttcagctt agatctgagt
      241 ccggaggaat gagctactgc atcttctacc agctcagtaa tgttgccttc ggttttcctg
      301 acgaggcgat ggctgatgta atggcttcct ggcattttaa gaatcttttt tgccgccttg
      361 taagtagtct tgaattggca tttgtgattg ccgcctcctt caagttttag gtacatcgta
      421 acatcaccct tcagaactcc gtcgctggca gtaattttct cggttgatgg ctcccaatca
      481 acactttggt tttgcatgat aggaccatcg gcaggaaagt taaccccagt aaatttggat
      541 ttgtggtaga aggtgtttcc tctaaggctt atatgcgcac tgactgaagc ggacccacca
      601 tcttcgaact ccaacgacct ttcccatgac aggccttcag gaaatgcttg tttgaaatag
      661 tctggtatct cttctggata tttagtaaaa ggtctgtggc cgtaacagaa cacgtgtgac
      721 actaagtcaa acgcgaaagg cattggcccg cccttggcca ttgtgacgcg tagtgtcatc
      781 tcttgatgtc cctcgtaagg tctgcctgtg ccttcacctt caattgtgaa ctcatgccca
      841 ttgacggagc cgtccatgta gtacctcatc ttcatctctg gtttaatcac actcaccatg
      901 gtaccggtag cgctagcgga tctgacggtt cactaaacca gctctgctta tatagacctc
      961 ccaccgtaca cgcctaccgc ccatt
//
