LOCUS       sequence-101910-         929 bp    DNA     linear   UNA 15-DEC-2025
DEFINITION  Standardized mini-Tn5 delivery plasmid, kanamycin resistant.
ACCESSION   .
VERSION     .
KEYWORDS    .
SOURCE      natural DNA sequence
  ORGANISM  unspecified
REFERENCE   1  (bases 1 to 929)
  AUTHORS   Martinez-Garcia E, Aparicio T, de Lorenzo V, Nikel PI
  TITLE     New transposon tools tailored for metabolic engineering of 
            gram-negative microbial cell factories.
  JOURNAL   Front Bioeng Biotechnol. 2014 Oct 28;2:46. doi: 
            10.3389/fbioe.2014.00046. eCollection 2014.
  PUBMED    25389526
REFERENCE   2  (bases 1 to 929)
  AUTHORS   .
  TITLE     Direct Submission
  JOURNAL   Exported Dec 15, 2025 from SnapGene Server 8.0.1
            https://www.snapgene.com
COMMENT     SGRef: number: 1; type: "Journal Article"; journalName: "Front 
            Bioeng Biotechnol."; date: "2014-10-28"; volume: "2:46. doi"; pages:
            " 10.3389/fbioe.2014.00046. eCollection 2014"
COMMENT     Sequence Label: pBAMD1-2
FEATURES             Location/Qualifiers
     source          1..929
                     /mol_type="genomic DNA"
                     /organism="unspecified"
     misc_feature    39..57
                     /label=Tn5 ME
                     /note="hyperactive mosaic end for Tn5 transposase 
                     recognition (Reznikoff et al., 2004)"
ORIGIN
        1 gtcattactg gatctatcaa caggagtcca agactagtct gtctcttata cacatcttca
       61 gagattttat gcttgtaaac cgttttgtga aaaaattttt aaaataaaaa aggggacctc
      121 tagggtccgc aattaattag acaagggtct tagaaaaatt catccagcat cagatgaaat
      181 tgcagtttgt tcatatccgg attatcaatg ccatatttct gaaacagacg tttttgcagg
      241 ctcgggctaa attcgcccag gcagttccac agaatggcca gatcctgata acgatccgca
      301 atgcccacac ggcccacatc aatgcagcca atcagtttgc cttcatcgaa aatcaggtta
      361 tccaggctaa aatcgccgtg ggtcaccacg ctatccgggc taaacggcag cagtttatgc
      421 atttctttcc acacctgttc caccggccag ccgttacgtt catcatcaaa atcgctcgca
      481 tccaccaggc cgttgttcat acggctctgc gcctgggcca gacgaaacac acgatcgctg
      541 ttaaacgggc agttgcacac cggaatgcta tgcagacgac gcagaaacac ggccagcgca
      601 tccacaatgt tttcgccgct atccggatat tcttccagca cctgaaacgc ggttttgccc
      661 ggaatcgcgg tggtcagcag ccacgcatca tccggggtgc gaataaaatg tttaatggtc
      721 ggcagcggca taaattcggt cagccagttc agacgcacca tttcatcggt cacatcgttc
      781 gccacgctgc ctttgccatg tttcagaaac agttccggcg catccggttt gccatacaga
      841 cgataaatgg tcgcgccgct ctgacccacg ttatcacgcg cccatttata gccatacaga
      901 tccgcatcca tgttgctgtt cagacgcgg
//
