LOCUS       sequence-101915-         828 bp    DNA     linear   UNA 15-DEC-2025
DEFINITION  Expresses human OTUD1 (OTU domain) in E. coli..
ACCESSION   .
VERSION     .
KEYWORDS    .
SOURCE      natural DNA sequence
  ORGANISM  unspecified
REFERENCE   1  (bases 1 to 828)
  AUTHORS   Mevissen TE, Hospenthal MK, Geurink PP, Elliott PR, Akutsu M, 
            Arnaudo N, Ekkebus R, Kulathu Y, Wauer T, El Oualid F, Freund SM, 
            Ovaa H, Komander D
  TITLE     OTU Deubiquitinases Reveal Mechanisms of Linkage Specificity and 
            Enable Ubiquitin Chain Restriction Analysis.
  JOURNAL   Cell. 2013 Jul 3;154(1):169-84. doi: 10.1016/j.cell.2013.05.046.
  PUBMED    23827681
REFERENCE   2  (bases 1 to 828)
  AUTHORS   .
  TITLE     Direct Submission
  JOURNAL   Exported Dec 15, 2025 from SnapGene Server 8.0.1
            https://www.snapgene.com
COMMENT     SGRef: number: 1; type: "Journal Article"; doi: 
            "10.1016/j.cell.2013.05"; journalName: "Cell"; date: "2013-07-3- 3";
            volume: "154"; issue: "1"; pages: "169-84"
COMMENT     Sequence Label: pOPINK-OTUD1 (OTU, aa 287-437)
FEATURES             Location/Qualifiers
     source          1..828
                     /mol_type="genomic DNA"
                     /organism="unspecified"
     primer_bind     232..254
                     /label=pGEX 5'
                     /note="pGEX vectors, Glutathione-S-transferase, forward 
                     primer"
     CDS             293..316
                     /codon_start=1
                     /product="recognition and cleavage site for human 
                     rhinovirus 3C and PreScission proteases"
                     /label=HRV 3C site
                     /translation="LEVLFQGP"
     CDS             793..810
                     /codon_start=1
                     /product="6xHis affinity tag"
                     /label=6xHis
                     /translation="HHHHHH"
ORIGIN
        1 tgaaatgctg aaaatgttcg aagatcgttt atgtcataaa acatatttaa atggtgatca
       61 tgtaacccat cctgacttca tgttgtatga cgctcttgat gttgttttat acatggaccc
      121 aatgtgcctg gatgcgttcc caaaattagt ttgttttaaa aaacgtattg aagctatccc
      181 acaaattgat aagtacttga aatccagcaa gtatatagca tggcctttgc agggctggca
      241 agccacgttt ggtggtggcg accatcctcc aaaatcggat ctgagcagcg gtctggaagt
      301 tctgtttcag ggcccggacg agaagctggc cctatacctg gccgaggtgg agaagcagga
      361 caagtatctg cggcagagga ataagtaccg attccacatc attccagacg gcaactgcct
      421 ctaccgagct gtcagcaaga cggtgtatgg ggaccagagc ctgcaccggg agttgaggga
      481 gcagacggtg cactacatcg ccgaccatct cgaccacttc agccccctga ttgagggcga
      541 cgtgggggag tttatcatcg ctgctgccca agacggggca tgggccgggt acccggagtt
      601 gctggccatg gggcagatgc tgaatgtgaa tatccattta actactggag ggaggctgga
      661 gagtcccacg gtgtctacca tgattcatta tttgggccca gaggattccc tgaggcctag
      721 tatttggctc agttggctca gtaacggaca ctatgatgct gtatttgatt aaagctttct
      781 agaccattta aacaccacca ccaccaccac tgagatccgg ctgctaac
//
