LOCUS       sequence-101917-         826 bp    DNA     linear   UNA 15-DEC-2025
DEFINITION  Expresses human Cezanne2 (OTU+UBA domains) in E. coli..
ACCESSION   .
VERSION     .
KEYWORDS    .
SOURCE      natural DNA sequence
  ORGANISM  unspecified
REFERENCE   1  (bases 1 to 826)
  AUTHORS   Mevissen TE, Hospenthal MK, Geurink PP, Elliott PR, Akutsu M, 
            Arnaudo N, Ekkebus R, Kulathu Y, Wauer T, El Oualid F, Freund SM, 
            Ovaa H, Komander D
  TITLE     OTU Deubiquitinases Reveal Mechanisms of Linkage Specificity and 
            Enable Ubiquitin Chain Restriction Analysis.
  JOURNAL   Cell. 2013 Jul 3;154(1):169-84. doi: 10.1016/j.cell.2013.05.046.
  PUBMED    23827681
REFERENCE   2  (bases 1 to 826)
  AUTHORS   .
  TITLE     Direct Submission
  JOURNAL   Exported Dec 15, 2025 from SnapGene Server 8.0.1
            https://www.snapgene.com
COMMENT     SGRef: number: 1; type: "Journal Article"; doi: 
            "10.1016/j.cell.2013.05"; journalName: "Cell"; date: "2013-07-3- 3";
            volume: "154"; issue: "1"; pages: "169-84"
COMMENT     Sequence Label: pOPINK-Cezanne2 (OTU+UBA, aa 1-462)
FEATURES             Location/Qualifiers
     source          1..826
                     /mol_type="genomic DNA"
                     /organism="unspecified"
     CDS             791..808
                     /codon_start=1
                     /product="6xHis affinity tag"
                     /label=6xHis
                     /translation="HHHHHH"
ORIGIN
        1 atgggaactg ccttttacat gctgcttcac tgggaatgtg ggggtttcac gaccgggacc
       61 tggtgttacg gaaagctctc tataccatga tgaggacggg agctgagagg gaagccctga
      121 agcggaggtg gaggtggcag cagacgcagc agaataagga gtcagggctg gtgtacacag
      181 aggaggagtg ggagcgggag tggacggagc tgctgaagct ggcctccagc gagccgcgca
      241 cacacttcag caagaatggc ggcacgggcg ggggtgtgga caactctgag gaccccgtgt
      301 acgagagcct ggaagagttc cacgtttttg tcctagccca tatattaaga aggcccatcg
      361 ttgttgtggc agatacaatg ttaagagact caggtggaga agcgttcgca cccatcccat
      421 tcggagggat ctacctgccc ttggaggtcc ctcccaacag atgccactgc tcgcctctgg
      481 ttctggccta tgatcaagcc catttctctg cccttgtgtc catggaacag agagaccagc
      541 aaagagaaca agccgtgatc cccctgacgg attctgagca caagctgctg cctctgcact
      601 ttgcagtgga ccctggcaag gactgggagt gggggaaaga cgacaacgat aacgcccggc
      661 tggcccacct tatcctgtcg ctagaagcca agctgaacct tctgcacagc tacatgaacg
      721 tgacgtggat ccggatcccc tccgagacac gggcgcccct ggcacagtaa agctttctag
      781 accatttaaa caccaccacc accaccactg agatccggct gctaac
//
