LOCUS       sequence-101919-         781 bp    DNA     linear   UNA 15-DEC-2025
DEFINITION  Expresses human OTUD3 (OTU domain) in E. coli..
ACCESSION   .
VERSION     .
KEYWORDS    .
SOURCE      natural DNA sequence
  ORGANISM  unspecified
REFERENCE   1  (bases 1 to 781)
  AUTHORS   Mevissen TE, Hospenthal MK, Geurink PP, Elliott PR, Akutsu M, 
            Arnaudo N, Ekkebus R, Kulathu Y, Wauer T, El Oualid F, Freund SM, 
            Ovaa H, Komander D
  TITLE     OTU Deubiquitinases Reveal Mechanisms of Linkage Specificity and 
            Enable Ubiquitin Chain Restriction Analysis.
  JOURNAL   Cell. 2013 Jul 3;154(1):169-84. doi: 10.1016/j.cell.2013.05.046.
  PUBMED    23827681
REFERENCE   2  (bases 1 to 781)
  AUTHORS   .
  TITLE     Direct Submission
  JOURNAL   Exported Dec 15, 2025 from SnapGene Server 8.0.1
            https://www.snapgene.com
COMMENT     SGRef: number: 1; type: "Journal Article"; doi: 
            "10.1016/j.cell.2013.05"; journalName: "Cell"; date: "2013-07-3- 3";
            volume: "154"; issue: "1"; pages: "169-84"
COMMENT     Sequence Label: pOPINK-OTUD3 (OTU, aa 52-209)
FEATURES             Location/Qualifiers
     source          1..781
                     /mol_type="genomic DNA"
                     /organism="unspecified"
     CDS             33..50
                     /codon_start=1
                     /product="6xHis affinity tag"
                     /label=6xHis
                     /translation="HHHHHH"
     CDS             51..704
                     /codon_start=1
                     /product="glutathione S-transferase from Schistosoma 
                     japonicum"
                     /label=GST
                     /translation="MSPILGYWKIKGLVQPTRLLLEYLEEKYEEHLYERDEGDKWRNKK
                     FELGLEFPNLPYYIDGDVKLTQSMAIIRYIADKHNMLGGCPKERAEISMLEGAVLDIRY
                     GVSRIAYSKDFETLKVDFLSKLPEMLKMFEDRLCHKTYLNGDHVTHPDFMLYDALDVVL
                     YMDPMCLDAFPKLVCFKKRIEAIPQIDKYLKSSKYIAWPLQGWQATFGGGDHPPK"
     primer_bind     662..684
                     /label=pGEX 5'
                     /note="pGEX vectors, Glutathione-S-transferase, forward 
                     primer"
     CDS             723..746
                     /codon_start=1
                     /product="recognition and cleavage site for human 
                     rhinovirus 3C and PreScission proteases"
                     /label=HRV 3C site
                     /translation="LEVLFQGP"
ORIGIN
        1 gtttaacttt aagaaggaga tataccatgg cacatcacca tcaccatcac atgtccccta
       61 tactaggtta ttggaaaatt aagggccttg tgcaacccac tcgacttctt ttggaatatc
      121 ttgaagaaaa atatgaagag catttgtatg agcgcgatga aggtgataaa tggcgaaaca
      181 aaaagtttga attgggtttg gagtttccca atcttcctta ttatattgat ggtgatgtta
      241 aattaacaca gtctatggcc atcatacgtt atatagctga caagcacaac atgttgggtg
      301 gttgtccaaa agagcgtgca gagatttcaa tgcttgaagg agcggttttg gatattagat
      361 acggtgtttc gagaattgca tatagtaaag actttgaaac tctcaaagtt gattttctta
      421 gcaagctacc tgaaatgctg aaaatgttcg aagatcgttt atgtcataaa acatatttaa
      481 atggtgatca tgtaacccat cctgacttca tgttgtatga cgctcttgat gttgttttat
      541 acatggaccc aatgtgcctg gatgcgttcc caaaattagt ttgttttaaa aaacgtattg
      601 aagctatccc acaaattgat aagtacttga aatccagcaa gtatatagca tggcctttgc
      661 agggctggca agccacgttt ggtggtggcg accatcctcc aaaatcggat ctgagcagcg
      721 gtctggaagt tctgtttcag ggcccggagt tcgtcagctt cgccaaccag ctgcaggccc
      781 t
//
