LOCUS       sequence-101920-         846 bp    DNA     linear   UNA 15-DEC-2025
DEFINITION  Expresses human OTUD3 (OTU domain) in E. coli..
ACCESSION   .
VERSION     .
KEYWORDS    .
SOURCE      natural DNA sequence
  ORGANISM  unspecified
REFERENCE   1  (bases 1 to 846)
  AUTHORS   Mevissen TE, Hospenthal MK, Geurink PP, Elliott PR, Akutsu M, 
            Arnaudo N, Ekkebus R, Kulathu Y, Wauer T, El Oualid F, Freund SM, 
            Ovaa H, Komander D
  TITLE     OTU Deubiquitinases Reveal Mechanisms of Linkage Specificity and 
            Enable Ubiquitin Chain Restriction Analysis.
  JOURNAL   Cell. 2013 Jul 3;154(1):169-84. doi: 10.1016/j.cell.2013.05.046.
  PUBMED    23827681
REFERENCE   2  (bases 1 to 846)
  AUTHORS   .
  TITLE     Direct Submission
  JOURNAL   Exported Dec 15, 2025 from SnapGene Server 8.0.1
            https://www.snapgene.com
COMMENT     SGRef: number: 1; type: "Journal Article"; doi: 
            "10.1016/j.cell.2013.05"; journalName: "Cell"; date: "2013-07-3- 3";
            volume: "154"; issue: "1"; pages: "169-84"
COMMENT     Sequence Label: pOPINK-OTUD3 (OTU, aa 52-209)
FEATURES             Location/Qualifiers
     source          1..846
                     /mol_type="genomic DNA"
                     /organism="unspecified"
     primer_bind     232..254
                     /label=pGEX 5'
                     /note="pGEX vectors, Glutathione-S-transferase, forward 
                     primer"
     CDS             293..316
                     /codon_start=1
                     /product="recognition and cleavage site for human 
                     rhinovirus 3C and PreScission proteases"
                     /label=HRV 3C site
                     /translation="LEVLFQGP"
     CDS             814..831
                     /codon_start=1
                     /product="6xHis affinity tag"
                     /label=6xHis
                     /translation="HHHHHH"
ORIGIN
        1 tgaaatgctg aaaatgttcg aagatcgttt atgtcataaa acatatttaa atggtgatca
       61 tgtaacccat cctgacttca tgttgtatga cgctcttgat gttgttttat acatggaccc
      121 aatgtgcctg gatgcgttcc caaaattagt ttgttttaaa aaacgtattg aagctatccc
      181 acaaattgat aagtacttga aatccagcaa gtatatagca tggcctttgc agggctggca
      241 agccacgttt ggtggtggcg accatcctcc aaaatcggat ctgagcagcg gtctggaagt
      301 tctgtttcag ggcccggagt tcgtcagctt cgccaaccag ctgcaggccc tggggctgaa
      361 gctgcgggag gtgccggggg acggcaattg cttgttcaga gctcttggtg atcaattgga
      421 gggacactca cgaaatcatc tcaagcacag acaggagaca gtggactaca tgataaagca
      481 gcgggaagat tttgaaccct ttgtagaaga tgacattcct tttgagaagc atgtggccag
      541 tttggcaaag cctggtactt ttgctggcaa tgatgcaatt gtagcctttg caagaaatca
      601 tcagttgaat gtagtgattc atcaacttaa tgcccctttg tggcagattc gtggtacaga
      661 gaaaagcagc gtgagggagt tacacatcgc atatcggtat ggagagcact acgacagtgt
      721 tcggaggatc aatgacaact cagaggcacc tgcacatctc cagacggatt ttcagatgct
      781 tcatcaagat taaagctttc tagaccattt aaacaccacc accaccacca ctgagatccg
      841 gctgct
//
