LOCUS       sequence-101925-         938 bp    DNA     linear   UNA 15-DEC-2025
DEFINITION  natural linear DNA
ACCESSION   .
VERSION     .
KEYWORDS    .
SOURCE      natural DNA sequence
  ORGANISM  unspecified
REFERENCE   1  (bases 1 to 938)
  TITLE     Davidson Orange Fluorescent Proteins
REFERENCE   2  (bases 1 to 938)
  AUTHORS   .
  TITLE     Direct Submission
  JOURNAL   Exported Dec 15, 2025 from SnapGene Server 8.0.1
            https://www.snapgene.com
COMMENT     SGRef: number: 1; type: "Journal Article"
COMMENT     Sequence Label: mKO2-Farnesyl-5
FEATURES             Location/Qualifiers
     source          1..938
                     /mol_type="genomic DNA"
                     /organism="unspecified"
     CDS             complement(244..897)
                     /codon_start=1
                     /product="monomeric Kusabira-Orange 2 fluorescent protein"
                     /label=mKO2
                     /translation="MVSVIKPEMKMRYYMDGSVNGHEFTIEGEGTGRPYEGHQEMTLRV
                     TMAEGGPMPFAFDLVSHVFCYGHRVFTKYPEEIPDYFKQAFPEGLSWERSLEFEDGGSA
                     SVSAHISLRGNTFYHKSKFTGVNFPADGPIMQNQSVDWEPSTEKITASDGVLKGDVTMY
                     LKLEGGGNHKCQMKTTYKAAKEILEMPGDHYIGHRLVRKTEGNITEQVEDAVAHS"
     regulatory      894..903
                     /label=Kozak sequence
                     /note="vertebrate consensus sequence for strong initiation 
                     of translation (Kozak, 1987)"
                     /regulatory_class="other"
ORIGIN
        1 aagttaacaa caacaattgc attcatttta tgtttcaggt tcagggggag gtgtgggagg
       61 ttttttaaag caagtaaaac ctctacaaat gtggtatggc tgattatgat cagttatcta
      121 gatccggtgg atcccgggcc cgcggtaccg tcgactgcag aattctcagg agagcacaca
      181 cttgcagctc atgcagccgg ggccactctc atcaggaggg ttcagcttag atctgagtcc
      241 ggagctatga gctactgcat cttctacctg ctcagtaatg ttgccttcgg ttttcctgac
      301 gaggcgatgg ccgatgtaat ggtctcctgg catttcaaga atctcttttg ccgccttgta
      361 agtagtcttc atttggcatt tgtgattgcc gcctccttca agttttaggt acatcgtaac
      421 atcacccttc agaactccgt cgctggcagt aattttctcg gttgatggct cccaatcaac
      481 actttggttt tgcatgatag gaccatcggc aggaaagtta accccagtaa atttggattt
      541 gtggtagaag gtgtttcctc taaggcttat atgcgcactg actgaagcgg acccaccatc
      601 ttcgaactcc aacgaccttt cccatgacag gccttcagga aatgcttgtt tgaaatagtc
      661 tggtatctct tctggatatt tagtaaatac tctgtggccg taacagaaca cgtgtgacac
      721 taagtcaaac gcgaaaggca ttggcccgcc ctcggccatt gtgacgcgta gtgtcatctc
      781 ttgatgtccc tcgtaaggtc tgcctgtgcc ttcaccttca attgtgaact catgcccatt
      841 gacggagccg tccatgtagt acctcatctt catctctggt ttaatcacac tcaccatggt
      901 ggcgaccggt agcgctagcg gatctgacgg ttcactaa
//
