LOCUS       sequence-101936-        1057 bp    DNA     linear   UNA 15-DEC-2025
DEFINITION  A human codon-optimized Cas9 from Staphylococcus aureus (SaCas9)..
ACCESSION   .
VERSION     .
KEYWORDS    .
SOURCE      natural DNA sequence
  ORGANISM  unspecified
REFERENCE   1  (bases 1 to 1057)
  AUTHORS   Ran FA, Cong L, Yan WX, Scott DA, Gootenberg JS, Kriz AJ, Zetsche B,
            Shalem O, Wu X, Makarova KS, Koonin EV, Sharp PA, Zhang F
  TITLE     In vivo genome editing using Staphylococcus aureus Cas9.
  JOURNAL   Nature. 2015 Apr 1. doi: 10.1038/nature14299.
  PUBMED    25830891
REFERENCE   2  (bases 1 to 1057)
  AUTHORS   .
  TITLE     Direct Submission
  JOURNAL   Exported Dec 15, 2025 from SnapGene Server 8.0.1
            https://www.snapgene.com
COMMENT     SGRef: number: 1; type: "Journal Article"; journalName: "Nature. 
            2015 Apr 1. doi: 10.1038/nature14299."
COMMENT     Sequence Label: pX600-AAV-CMV::NLS-SaCas9-NLS-3xHA-bGHpA
FEATURES             Location/Qualifiers
     source          1..1057
                     /mol_type="genomic DNA"
                     /organism="unspecified"
ORIGIN
        1 tctttcttgg tcttgctgat tctgcccttg cccttggcca gattcaggat gtgcttcttg
       61 aaggtttcgt agctgatctt gctgtcgctg ctgctcaggt actggaatgg ggtccggttg
      121 cccttcttgc tgttttcttc ctgcttcacg agcaccttgt tgttgaagct gttgtcgaag
      181 gacacgcttc tggggatgat gtggtccacc tcatagttga aggggttgtt cagcagatct
      241 tccagaggga tggcttccag gctgtacagg cacttgcctt cctgcatgtc gtgcagcttg
      301 atcttctcga tcaggtactt ggcgttctct ttgccggtgg tccggatgat ttcctcgatc
      361 cgctcgttgg tctgccggtt ccgcttctgc atctcgttga tcattttctg ggcgtccttg
      421 gagttcttct cgcgggccag ctcgataatg atgtcgttgg gcaggccgta cttcttgatg
      481 atggcgttga tcactttgat gctctggatg aagcttctct tcacgacggg gctcaggatg
      541 aagtcgtcca ccagggtggt ggggatctct ttctgctggg acaggtccac cttcttgggc
      601 accagcttca gccggttgaa gatagcgatc tggttgtcgt tggtgtgcca cagctcgtcc
      661 aggatcaggt tgatggcctt caggctcagg ttgtgggtgc cggtatagcc cttcagatta
      721 gagatctgct cgatctcttc ctgggtcagc tcggagttca gattggtcag ttcttcctgg
      781 atgtcctcgc tgctctggta gatggtcagg atcttggcaa tctgatccag cagctcggcg
      841 ttctcaataa tctctttccg ggcggtaatg tccttgatgt cgtggtacac cttcaggttg
      901 gtgaactcgg gcttgccggt gctggtcact ctgtagccct taatatcctc ttcgttcacg
      961 aggatttctt tggcgatctg cttcagggtg ggcttcttct tctgcttgaa cacgttctcg
     1021 atgatctgga acttctcgta atattccagc ttctcgt
//
