LOCUS       sequence-101937-        1028 bp    DNA     linear   UNA 15-DEC-2025
DEFINITION  bacterial expression of ranaspumin 2 as a fusion protein with the 
            fluorescent tag iLOV.
ACCESSION   .
VERSION     .
KEYWORDS    .
SOURCE      natural DNA sequence
  ORGANISM  unspecified
REFERENCE   1  (bases 1 to 1028)
  TITLE     Brian Smith Plasmids
  JOURNAL   Unpublished
REFERENCE   2  (bases 1 to 1028)
  AUTHORS   .
  TITLE     Direct Submission
  JOURNAL   Exported Dec 15, 2025 from SnapGene Server 8.0.1
            https://www.snapgene.com
COMMENT     SGRef: number: 1; type: "Journal Article"; journalName: 
            "Unpublished"
COMMENT     Sequence Label: pET28-iLOV-Rsn2
FEATURES             Location/Qualifiers
     source          1..1028
                     /mol_type="genomic DNA"
                     /organism="unspecified"
     primer_bind     68..87
                     /label=T7
                     /note="T7 promoter, forward primer"
     promoter        68..86
                     /label=T7 promoter
                     /note="promoter for bacteriophage T7 RNA polymerase"
     protein_bind    87..111
                     /label=lac operator
                     /bound_moiety="lac repressor encoded by lacI"
                     /note="The lac repressor binds to the lac operator to 
                     inhibit transcription in E. coli. This inhibition can be 
                     relieved by adding lactose or 
                     isopropyl-beta-D-thiogalactopyranoside (IPTG)."
     RBS             126..148
                     /note="efficient ribosome binding site from bacteriophage 
                     T7 gene 10 (Olins and Rangwala, 1989)"
     CDS             167..184
                     /codon_start=1
                     /product="6xHis affinity tag"
                     /label=6xHis
                     /translation="HHHHHH"
     CDS             194..211
                     /codon_start=1
                     /product="thrombin recognition and cleavage site"
                     /label=thrombin site
                     /translation="LVPRGS"
     CDS             899..916
                     /codon_start=1
                     /product="6xHis affinity tag"
                     /label=6xHis
                     /translation="HHHHHH"
     primer_bind     complement(969..987)
                     /label=T7 Term
                     /note="T7 terminator, reverse primer"
ORIGIN
        1 tgtggcgccg gtgatgccgg ccacgatgcg tccggcgtag aggatcgaga tctcgatccc
       61 gcgaaattaa tacgactcac tataggggaa ttgtgagcgg ataacaattc ccctctagaa
      121 ataattttgt ttaactttaa gaaggagata taccatgggc agcagccatc atcatcatca
      181 tcacagcagc ggcctggtgc cgcgcggcag ccatatgatc ggtaccattg agaagaattt
      241 cgtaatcact gatccacgtc tgccggacaa cccgatcatc ttcgcttccg acggcttcct
      301 ggagctgacc gagtattccc gtgaagagat cctgggccgt aacgcccgtt tcctgcaggg
      361 cccggagact gatcaggcga cggttcagaa aattcgcgat gctatccgtg accagcgtga
      421 aactacggtg caactgatta actacacgaa aagcggtaag aaattctgga acctgctgca
      481 tctgcaacca gtccgcgatc agaaaggcga gctgcagtat ttcatcggcg ttcaactgga
      541 tggttctgac cacgtacata tgttaatatt agatggggac ctactaaagg acaagttaaa
      601 gttaccggtc attgacaatc tgtttggtaa agaactcctt gacaagtttc aagatgatat
      661 taaggacaaa tatggggtgg acaccaaaga ccttaagatc ctcaagacat cagaagataa
      721 aaggttttac tatgtgtcgg tcgatgctgg agatggcgag aaatgtaaat tcaagattag
      781 aaaagatgtc gatgttccaa agatggtcgg acgcaaatgt aggaaggacg atgatgatga
      841 tgatggatat taggatccga attcgagctc cgtcgacaag cttgcggccg cactcgagca
      901 ccaccaccac caccactgag atccggctgc taacaaagcc cgaaaggaag ctgagttggc
      961 tgctgccacc gctgagcaat aactagcata accccttggg gcctctaaac gggtcttgag
     1021 gggttttt
//
