LOCUS       sequence-101938-        1024 bp    DNA     linear   UNA 15-DEC-2025
DEFINITION  bacterial expression of ranaspumin 2 as a fusion protein with the 
            fluorescent tag iLOV.
ACCESSION   .
VERSION     .
KEYWORDS    .
SOURCE      natural DNA sequence
  ORGANISM  unspecified
REFERENCE   1  (bases 1 to 1024)
  TITLE     Brian Smith Plasmids
  JOURNAL   Unpublished
REFERENCE   2  (bases 1 to 1024)
  AUTHORS   .
  TITLE     Direct Submission
  JOURNAL   Exported Dec 15, 2025 from SnapGene Server 8.0.1
            https://www.snapgene.com
COMMENT     SGRef: number: 1; type: "Journal Article"; journalName: 
            "Unpublished"
COMMENT     Sequence Label: pET28-iLOV-Rsn2
FEATURES             Location/Qualifiers
     source          1..1024
                     /mol_type="genomic DNA"
                     /organism="unspecified"
     CDS             complement(31..48)
                     /codon_start=1
                     /product="6xHis affinity tag"
                     /label=6xHis
                     /translation="HHHHHH"
     CDS             complement(736..753)
                     /codon_start=1
                     /product="thrombin recognition and cleavage site"
                     /label=thrombin site
                     /translation="LVPRGS"
     CDS             complement(763..780)
                     /codon_start=1
                     /product="6xHis affinity tag"
                     /label=6xHis
                     /translation="HHHHHH"
     RBS             799..821
                     /note="efficient ribosome binding site from bacteriophage 
                     T7 gene 10 (Olins and Rangwala, 1989)"
     protein_bind    836..860
                     /label=lac operator
                     /bound_moiety="lac repressor encoded by lacI"
                     /note="The lac repressor binds to the lac operator to 
                     inhibit transcription in E. coli. This inhibition can be 
                     relieved by adding lactose or 
                     isopropyl-beta-D-thiogalactopyranoside (IPTG)."
     primer_bind     complement(860..879)
                     /label=T7
                     /note="T7 promoter, forward primer"
     promoter        complement(861..879)
                     /label=T7 promoter
                     /note="promoter for bacteriophage T7 RNA polymerase"
     primer_bind     complement(963..982)
                     /label=pBRrevBam
                     /note="pBR322 vectors, tet region, downstream of BamHI, 
                     reverse primer"
ORIGIN
        1 ctttcgggct ttgttagcag ccggatctca gtggtggtgg tggtggtgct cgagtgcggc
       61 cgcaagcttg tcgacggagc tcgaattcgg atcctaatat ccatcatcat catcatcgtc
      121 cttcctacat ttgcgtccga ccatctttgg aacatcgaca tcttttctaa tcttgaattt
      181 acatttctcg ccatctccag catcgaccga cacatagtaa aaccttttat cttctgatgt
      241 cttgaggatc ttaaggtctt tggtgtccac cccatatttg tccttaatat catcttgaaa
      301 cttgtcaagg agttctttac caaacagatt gtcaatgacc ggtaacttta acttgtcctt
      361 tagtaggtcc ccatctaata ttaacatatg tacgtggtca gaaccatcca gttgaacgcc
      421 gatgaaatac tgcagctcgc ctttctgatc gcggactggt tgcagatgca gcaggttcca
      481 gaatttctta ccgcttttcg tgtagttaat cagttgcacc gtagtttcac gctggtcacg
      541 gatagcatcg cgaattttct gaaccgtcgc ctgatcagtc tccgggccct gcaggaaacg
      601 ggcgttacgg cccaggatct cttcacggga atactcggtc agctccagga agccgtcgga
      661 agcgaagatg atcgggttgt ccggcagacg tggatcagtg attacgaaat tcttctcaat
      721 ggtaccgatc atatggctgc cgcgcggcac caggccgctg ctgtgatgat gatgatgatg
      781 gctgctgccc atggtatatc tccttcttaa agttaaacaa aattatttct agaggggaat
      841 tgttatccgc tcacaattcc cctatagtga gtcgtattaa tttcgcggga tcgagatctc
      901 gatcctctac gccggacgca tcgtggccgg catcaccggc gccacaggtg cggttgctgg
      961 cgcctatatc gccgacatca ccgatgggga agatcgggct cgccacttcg ggctcatgag
     1021 cgct
//
