LOCUS       sequence-101941-        1030 bp    DNA     linear   UNA 15-DEC-2025
DEFINITION  bacterial expression of the horse sweat/saliva protein Latherin as a
            His-tagged thioredoxin fusion.
ACCESSION   .
VERSION     .
KEYWORDS    .
SOURCE      natural DNA sequence
  ORGANISM  unspecified
REFERENCE   1  (bases 1 to 1030)
  AUTHORS   Vance SJ, McDonald RE, Cooper A, Smith BO, Kennedy MW
  TITLE     The structure of latherin, a surfactant allergen protein from horse 
            sweat and saliva.
  JOURNAL   J R Soc Interface. 2013 Jun 19;10(85):20130453. doi: 
            10.1098/rsif.2013.0453. Print 2013 Aug 6.
  PUBMED    23782536
REFERENCE   2  (bases 1 to 1030)
  AUTHORS   .
  TITLE     Direct Submission
  JOURNAL   Exported Dec 15, 2025 from SnapGene Server 8.0.1
            https://www.snapgene.com
COMMENT     SGRef: number: 1; type: "Journal Article"; journalName: "J R Soc 
            Interface."; date: "2013-06-19"; volume: "10(85):20130453. doi"; 
            pages: " 10.1098/rsif.2013.0453. Print 2013 Aug 6"
COMMENT     Sequence Label: pET32-sLatherin
FEATURES             Location/Qualifiers
     source          1..1030
                     /mol_type="genomic DNA"
                     /organism="unspecified"
     primer_bind     74..93
                     /label=T7
                     /note="T7 promoter, forward primer"
     promoter        74..92
                     /label=T7 promoter
                     /note="promoter for bacteriophage T7 RNA polymerase"
     protein_bind    93..117
                     /label=lac operator
                     /bound_moiety="lac repressor encoded by lacI"
                     /note="The lac repressor binds to the lac operator to 
                     inhibit transcription in E. coli. This inhibition can be 
                     relieved by adding lactose or 
                     isopropyl-beta-D-thiogalactopyranoside (IPTG)."
     RBS             132..154
                     /note="efficient ribosome binding site from bacteriophage 
                     T7 gene 10 (Olins and Rangwala, 1989)"
     CDS             162..488
                     /codon_start=1
                     /gene="trxA"
                     /product="E. coli thioredoxin"
                     /label=TrxA
                     /translation="MSDKIIHLTDDSFDTDVLKADGAILVDFWAEWCGPCKMIAPILDE
                     IADEYQGKLTVAKLNIDQNPGTAPKYGIRGIPTLLLFKNGEVAATKVGALSKGQLKEFL
                     DANLA"
     CDS             510..527
                     /codon_start=1
                     /product="6xHis affinity tag"
                     /label=6xHis
                     /translation="HHHHHH"
     CDS             537..554
                     /codon_start=1
                     /product="thrombin recognition and cleavage site"
                     /label=thrombin site
                     /translation="LVPRGS"
     CDS             561..605
                     /codon_start=1
                     /product="affinity and epitope tag derived from pancreatic 
                     ribonuclease A"
                     /label=S-Tag
                     /translation="KETAAAKFERQHMDS"
     CDS             621..635
                     /codon_start=1
                     /product="enterokinase recognition and cleavage site"
                     /label=enterokinase site
                     /translation="DDDDK"
ORIGIN
        1 cctgtggcgc cggtgatgcc ggccacgatg cgtccggcgt agaggatcga gatcgatctc
       61 gatcccgcga aattaatacg actcactata ggggaattgt gagcggataa caattcccct
      121 ctagaaataa ttttgtttaa ctttaagaag gagatataca tatgagcgat aaaattattc
      181 acctgactga cgacagtttt gacacggatg tactcaaagc ggacggggcg atcctcgtcg
      241 atttctgggc agagtggtgc ggtccgtgca aaatgatcgc cccgattctg gatgaaatcg
      301 ctgacgaata tcagggcaaa ctgaccgttg caaaactgaa catcgatcaa aaccctggca
      361 ctgcgccgaa atatggcatc cgtggtatcc cgactctgct gctgttcaaa aacggtgaag
      421 tggcggcaac caaagtgggt gcactgtcta aaggtcagtt gaaagagttc ctcgacgcta
      481 acctggccgg ttctggttct ggccatatgc accatcatca tcatcattct tctggtctgg
      541 tgccacgcgg ttctggtatg aaagaaaccg ctgctgctaa attcgaacgc cagcacatgg
      601 acagcccaga tctgggtacc gacgacgacg acaaggccat ggctcaacaa attccgccgg
      661 aagtgagcag ccagattacc gatgcgctga cccagggcct gctggatggc aactttctga
      721 gcctgctgaa cgcgattaac ctggaaggtc tgctgaacac cattctggat caggtgaccg
      781 gcctgctgaa tattctggtg ggcccgctgc tgggtccgtc tgatgcggaa attaaactgc
      841 aggatacccg tctgctgcaa ctgtctctgg aatttagccc ggatagcaaa ggcattgata
      901 tttggattcc gctggaactg tctgtgtatc tgaaactgct gattctggaa ccgctgaccc
      961 tgtatgtgcg taccgatatt cgtgtgcagc tgcgtctgga atctgatgaa gatggcaaat
     1021 atcgtctggc
//
