LOCUS       sequence-101944-         873 bp    DNA     linear   UNA 15-DEC-2025
DEFINITION  Expresses a fusion protein of FKBP12(F36V)-linker peptide 2- 
            Cre(19-59).
ACCESSION   .
VERSION     .
KEYWORDS    .
SOURCE      natural DNA sequence
  ORGANISM  unspecified
REFERENCE   1  (bases 1 to 873)
  TITLE     Herman's lab DiCre2-59.F2 plasmid
  JOURNAL   Unpublished
REFERENCE   2  (bases 1 to 873)
  AUTHORS   .
  TITLE     Direct Submission
  JOURNAL   Exported Dec 15, 2025 from SnapGene Server 8.0.1
            https://www.snapgene.com
COMMENT     SGRef: number: 1; type: "Journal Article"; journalName: 
            "Unpublished"
COMMENT     Sequence Label: pcDNA_DiCre2_59.F2
FEATURES             Location/Qualifiers
     source          1..873
                     /mol_type="genomic DNA"
                     /organism="unspecified"
     primer_bind     53..72
                     /label=T7
                     /note="T7 promoter, forward primer"
     promoter        53..71
                     /label=T7 promoter
                     /note="promoter for bacteriophage T7 RNA polymerase"
     regulatory      139..148
                     /label=Kozak sequence
                     /note="vertebrate consensus sequence for strong initiation 
                     of translation (Kozak, 1987)"
                     /regulatory_class="other"
     CDS             151..171
                     /codon_start=1
                     /product="nuclear localization signal of SV40 (simian virus
                     40) large T antigen"
                     /label=SV40 NLS
                     /translation="PKKKRKV"
     CDS             178..498
                     /codon_start=1
                     /product="F36V mutant of FK506-binding protein FKBP12"
                     /label=FKBP-V
                     /note="binds synthetic ligands that do not bind wild-type 
                     FKBP"
                     /translation="GVQVETISPGDGRTFPKRGQTCVVHYTGMLEDGKKVDSSRDRNKP
                     FKFMLGKQEVIRGWEEGVAQMSVGQRAKLTISPDYAYGATGHPGIIPPHATLVFDVELL
                     KLE"
     primer_bind     complement(698..715)
                     /label=BGH-rev
                     /note="Bovine growth hormone terminator, reverse primer. 
                     Also called BGH reverse"
     polyA_signal    704..815
                     /label=bGH poly(A) signal
                     /note="bovine growth hormone polyadenylation signal"
ORIGIN
        1 gcagagctct ctggctaact agagaaccca ctgcttactg gcttatcgaa attaatacga
       61 ctcactatag ggagacccaa gctggctagg atcgatccac tagtccagtg tggtggaatt
      121 cctgcagccc gggggatcgc caccatggcc cctaagaaga agagaaaggt gtctagagga
      181 gtgcaggtgg aaaccatctc cccaggagac gggcgcacct tccccaagcg cggccagacc
      241 tgcgtggtgc actacaccgg gatgcttgaa gatggaaaga aagttgattc ctcccgggac
      301 agaaacaagc cctttaagtt tatgctaggc aagcaggagg tgatccgagg ctgggaagaa
      361 ggggttgccc agatgagtgt gggtcagaga gccaaactga ctatatctcc agattatgcc
      421 tatggtgcca ctgggcaccc aggcatcatc ccaccacatg ccactctcgt cttcgatgtg
      481 gagcttctaa aactggaagc tagccccagc aaccccggcg ccagcaacgg atccacctct
      541 gatgaagtca ggaagaacct gatggacatg ttcagggaca ggcaggcctt ctctgaacac
      601 acctggaaga tgctcctgtc tgtgtgcaga tcctgggctg cctggtgcaa gctgaactga
      661 ctcgagtcta gagggcccgt ttaaacccgc tgatcagcct cgactgtgcc ttctagttgc
      721 cagccatctg ttgtttgccc ctcccccgtg ccttccttga ccctggaagg tgccactccc
      781 actgtccttt cctaataaaa tgaggaaatt gcatcgcatt gtctgagtag gtgtcattct
      841 attctggggg gtggggtggg gcaggacagc aag
//
