LOCUS       sequence-101949-         878 bp    DNA     linear   UNA 15-DEC-2025
DEFINITION  Expresses a fusion protein of FKBP12-linker peptide 2- Cre(19-59).
ACCESSION   .
VERSION     .
KEYWORDS    .
SOURCE      natural DNA sequence
  ORGANISM  unspecified
REFERENCE   1  (bases 1 to 878)
  AUTHORS   Jullien N, Sampieri F, Enjalbert A, Herman JP
  TITLE     Regulation of Cre recombinase by ligand-induced complementation of 
            inactive fragments.
  JOURNAL   Nucleic Acids Res. 2003 Nov 1;31(21):e131.
  PUBMED    14576331
REFERENCE   2  (bases 1 to 878)
  AUTHORS   .
  TITLE     Direct Submission
  JOURNAL   Exported Dec 15, 2025 from SnapGene Server 8.0.1
            https://www.snapgene.com
COMMENT     SGRef: number: 1; type: "Journal Article"; journalName: "Nucleic 
            Acids Res."; date: "2003-11-1"; volume: "31(21)"; pages: "e131"
COMMENT     Sequence Label: pcDNA_DiCre_59.F2
FEATURES             Location/Qualifiers
     source          1..878
                     /mol_type="genomic DNA"
                     /organism="unspecified"
     primer_bind     66..83
                     /label=BGH-rev
                     /note="Bovine growth hormone terminator, reverse primer. 
                     Also called BGH reverse"
     CDS             complement(283..603)
                     /codon_start=1
                     /product="human FK506-binding protein FKBP12"
                     /label=FKBP
                     /translation="GVQVETISPGDGRTFPKRGQTCVVHYTGMLEDGKKFDSSRDRNKP
                     FKFMLGKQEVIRGWEEGVAQMSVGQRAKLTISPDYAYGATGHPGIIPPHATLVFDVELL
                     KLE"
     CDS             complement(610..630)
                     /codon_start=1
                     /product="nuclear localization signal of SV40 (simian virus
                     40) large T antigen"
                     /label=SV40 NLS
                     /translation="PKKKRKV"
     regulatory      633..642
                     /label=Kozak sequence
                     /note="vertebrate consensus sequence for strong initiation 
                     of translation (Kozak, 1987)"
                     /regulatory_class="other"
     primer_bind     complement(709..728)
                     /label=T7
                     /note="T7 promoter, forward primer"
     promoter        complement(710..728)
                     /label=T7 promoter
                     /note="promoter for bacteriophage T7 RNA polymerase"
     promoter        complement(773..872)
                     /label=CMVd1 promoter
                     /note="truncated human cytomegalovirus (CMV) immediate 
                     early promoter that yields slightly reduced expression 
                     relative to the full-length promoter"
     primer_bind     complement(802..822)
                     /label=CMV-F
                     /note="Human CMV immediate early promoter, forward primer"
ORIGIN
        1 gggagtggca ccttccaggg tcaaggaagg cacgggggag gggcaaacaa cagatggctg
       61 gcaactagaa ggcacagtcg aggctgatca gcgggtttaa acgggccctc tagactcgag
      121 tcagttcagc ttgcaccagg cagcccagga tctgcacaca gacaggagca tcttccaggt
      181 gtgttcagag aaggcctgcc tgtccctgaa catgtccatc aggttcttcc tgacttcatc
      241 agaggtggat ccgttgctgg cgccggggtt gctggggcta gcttccagtt ttagaagctc
      301 cacatcgaag acgagagtgg catgtggtgg gatgatgcct gggtgcccag tggcaccata
      361 ggcataatct ggagatatag tcagtttggc tctctgaccc acactcatct gggcaacccc
      421 ttcttcccag cctcggatca cctcctgctt gcctagcata aacttaaagg gcttgtttct
      481 gtcccgggag gaatcaaatt tctttccatc ttcaagcatc ccggtgtagt gcaccacgca
      541 ggtctggccg cgcttgggga aggtgcgccc gtctcctggg gagatggttt ccacctgcac
      601 tcctctagac acctttctct tcttcttagg ggccatggtg gcgatccccc gggctgcagg
      661 aattccacca cactggacta gtggatcgat cctagccagc ttgggtctcc ctatagtgag
      721 tcgtattaat ttcgataagc cagtaagcag tgggttctct agttagccag agagctctgc
      781 ttatatagac ctcccaccgt acacgcctac cgcccatttg cgtcaatggg gcggagttgt
      841 tacgacattt tggaaagtcc cgttgatttt ggtgccaa
//
