LOCUS       sequence-101967-         892 bp    DNA     linear   UNA 15-DEC-2025
DEFINITION  N -terminal His tagged wild-type GUS expression vector.
ACCESSION   .
VERSION     .
KEYWORDS    .
SOURCE      natural DNA sequence
  ORGANISM  unspecified
REFERENCE   1  (bases 1 to 892)
  AUTHORS   Matsumura I, Wallingford JB, Surana NK, Vize PD, Ellington AD
  TITLE     Directed evolution of the surface chemistry of the reporter enzyme 
            beta-glucuronidase.
  JOURNAL   Nat Biotechnol. 1999 Jul;17(7):696-701.
  PUBMED    10404164
REFERENCE   2  (bases 1 to 892)
  AUTHORS   .
  TITLE     Direct Submission
  JOURNAL   Exported Dec 15, 2025 from SnapGene Server 8.0.1
            https://www.snapgene.com
COMMENT     SGRef: number: 1; type: "Journal Article"; journalName: "Nat 
            Biotechnol."; date: "1999-07"; volume: "17(7)"; pages: "696-701"
COMMENT     Sequence Label: 6his-WT gusA-pET28a+
FEATURES             Location/Qualifiers
     source          1..892
                     /mol_type="genomic DNA"
                     /organism="unspecified"
     primer_bind     53..72
                     /label=T7
                     /note="T7 promoter, forward primer"
     promoter        53..71
                     /label=T7 promoter
                     /note="promoter for bacteriophage T7 RNA polymerase"
     protein_bind    72..96
                     /label=lac operator
                     /bound_moiety="lac repressor encoded by lacI"
                     /note="The lac repressor binds to the lac operator to 
                     inhibit transcription in E. coli. This inhibition can be 
                     relieved by adding lactose or 
                     isopropyl-beta-D-thiogalactopyranoside (IPTG)."
     RBS             111..133
                     /note="efficient ribosome binding site from bacteriophage 
                     T7 gene 10 (Olins and Rangwala, 1989)"
     CDS             152..169
                     /codon_start=1
                     /product="6xHis affinity tag"
                     /label=6xHis
                     /translation="HHHHHH"
     CDS             179..196
                     /codon_start=1
                     /product="thrombin recognition and cleavage site"
                     /label=thrombin site
                     /translation="LVPRGS"
ORIGIN
        1 gccggccacg atgcgtccgg cgtagaggat cgagatctcg atcccgcgaa attaatacga
       61 ctcactatag gggaattgtg agcggataac aattcccctc tagaaataat tttgtttaac
      121 tttaagaagg agatatacca tgggcagcag ccatcatcat catcatcaca gcagcggcct
      181 ggtgccgcgc ggcagccata tgttacgtcc tgtagaaacc ccaacccgtg aaatcaaaaa
      241 actcgacggc ctgtgggcat tcagtctgga tcgcgaaaac tgtggaattg atcagcgttg
      301 gtgggaaagc gcgttacaag aaagccgggc aattgctgtg ccaggcagtt ttaacgatca
      361 gttcgccgat gcagatattc gtaattatgc gggcaacgtc tggtatcagc gcgaagtctt
      421 tataccgaaa ggttgggcag gccagcgtat cgtgctgcgt ttcgatgcgg tcactcatta
      481 cggcaaagtg tgggtcaata atcaggaagt gatggagcat cagggcggct atacgccatt
      541 tgaagccgat gtcacgccgt atgttattgc cgggaaaagt gtacgtatca ccgtttgtgt
      601 gaacaacgaa ctgaactggc agactatccc gccgggaatg gtgattaccg acgaaaacgg
      661 caagaaaaag cagtcttact tccatgattt ctttaactat gccgggatcc atcgcagcgt
      721 aatgctctac accacgccga acacctgggt ggacgatatc accgtggtga cgcatgtcgc
      781 gcaagactgt aaccacgcgt ctgttgactg gcaggtggtg gccaatggtg atgtcagcgt
      841 tgaactgcgt gatgcggatc aacaggtggt tgcaactgga caaggcacta gc
//
