LOCUS       sequence-101970-         749 bp    DNA     linear   UNA 15-DEC-2025
DEFINITION  N -terminal His tagged wild-type GUS expression vector.
ACCESSION   .
VERSION     .
KEYWORDS    .
SOURCE      natural DNA sequence
  ORGANISM  unspecified
REFERENCE   1  (bases 1 to 749)
  AUTHORS   Matsumura I, Wallingford JB, Surana NK, Vize PD, Ellington AD
  TITLE     Directed evolution of the surface chemistry of the reporter enzyme 
            beta-glucuronidase.
  JOURNAL   Nat Biotechnol. 1999 Jul;17(7):696-701.
  PUBMED    10404164
REFERENCE   2  (bases 1 to 749)
  AUTHORS   .
  TITLE     Direct Submission
  JOURNAL   Exported Dec 15, 2025 from SnapGene Server 8.0.1
            https://www.snapgene.com
COMMENT     SGRef: number: 1; type: "Journal Article"; journalName: "Nat 
            Biotechnol."; date: "1999-07"; volume: "17(7)"; pages: "696-701"
COMMENT     Sequence Label: 6his-WT gusA-pET28a+
FEATURES             Location/Qualifiers
     source          1..749
                     /mol_type="genomic DNA"
                     /organism="unspecified"
     CDS             complement(17..34)
                     /codon_start=1
                     /product="6xHis affinity tag"
                     /label=6xHis
                     /translation="HHHHHH"
ORIGIN
        1 tagcagccgg atctcagtgg tggtggtggt ggtgctcgag tgcggccgca agcttgtcga
       61 cggagctcga attctgcagt cattgtttgc ctccctgctg cggtttttca ccgaagttca
      121 tgccagtcca gcgtttttgc agcagaaaag ccgccgactt cggtttgcgg tcgcgagtga
      181 agatcccttt cttgttaccg ccaacgcgca atatgccttg cgaggtcgca aaatcggcga
      241 aattccatac ctgttcaccg acgacggcgc tgacgcgatc aaagacgcgg tgatacatat
      301 ccagccatgc acactgatac tcttcactcc acatgtcggt gtacattgag tgcagcccgg
      361 ctaacgtatc cacgccgtat tcggtgatga taatcggctg atgcagtttc tcctgccagg
      421 ccagaagttc tttttccagt accttctctg ccgtttccaa atcgccgctt tggacatacc
      481 atccgtaata acggttcagg cacagcacat caaagagatc gctgatggta tcggtgtgag
      541 cgtcgcagaa cattacattg acgcaggtga tcggacgcgt cgggtcgagt ttacgcgttg
      601 cttccgccag tggcgcgaaa tattcccgtg caccttgcgg acgggtatcc ggttcgttgg
      661 caatactcca catcaccacg cttgggtggt ttttgtcacg cgctatcagc tctttaatcg
      721 cctgtaagtg cgcttgctga gtttccccg
//
