LOCUS       sequence-101971-         457 bp    DNA     linear   UNA 15-DEC-2025
DEFINITION  N-terminal His tagged round 3 aldehyde-resistant GUS expression 
            vector.
ACCESSION   .
VERSION     .
KEYWORDS    .
SOURCE      natural DNA sequence
  ORGANISM  unspecified
REFERENCE   1  (bases 1 to 457)
  AUTHORS   Matsumura I, Wallingford JB, Surana NK, Vize PD, Ellington AD
  TITLE     Directed evolution of the surface chemistry of the reporter enzyme 
            beta-glucuronidase.
  JOURNAL   Nat Biotechnol. 1999 Jul;17(7):696-701.
  PUBMED    10404164
REFERENCE   2  (bases 1 to 457)
  AUTHORS   .
  TITLE     Direct Submission
  JOURNAL   Exported Dec 15, 2025 from SnapGene Server 8.0.1
            https://www.snapgene.com
COMMENT     SGRef: number: 1; type: "Journal Article"; journalName: "Nat 
            Biotechnol."; date: "1999-07"; volume: "17(7)"; pages: "696-701"
COMMENT     Sequence Label: 6his R.3-AR GUSpET28a+/pLysS
FEATURES             Location/Qualifiers
     source          1..457
                     /mol_type="genomic DNA"
                     /organism="unspecified"
     CDS             complement(16..33)
                     /codon_start=1
                     /product="6xHis affinity tag"
                     /label=6xHis
                     /translation="HHHHHH"
ORIGIN
        1 agcagccgga tctcagtggt ggtggtggtg gtgctcgagt gcggccgcaa gcttgtcgac
       61 ggagctcgaa ttctgcagtc attgtttgcc tccctgctgc ggtttttcac cgaagttcat
      121 gccagtccag cgtttttgca gcagaaaagc cgccgacttc ggtttgcggt cgcgagtgaa
      181 gatccctttc ctgttaccgc caacgcgcaa tatgccttgc gaggtcgcaa aatcggcgaa
      241 attccatacc tgttcaccga cgacggcgct gacgcgatca aagacgcggt gatacatatc
      301 cagccatgca cactgatact cttcactcca catgtcggtg tacattgagt gcagcccggc
      361 taacgtttcc acgccgtatt cggtgatgat aatcggccga tgcagtttct cctgccaggc
      421 cagaagttct ttttccagta ccttctctgc cgcttcc
//
