LOCUS       sequence-101972-        9540 bp    DNA     circular SYN 15-DEC-2025
DEFINITION  human BRN3A cDNA.
ACCESSION   .
VERSION     .
KEYWORDS    .
SOURCE      synthetic DNA construct
  ORGANISM  synthetic DNA construct
REFERENCE   1  (bases 1 to 9540)
  AUTHORS   Blanchard JW, Eade KT, Szucs A, Lo Sardo V, Tsunemoto RK, Williams 
            D, Sanna PP, Baldwin KK
  TITLE     Selective conversion of fibroblasts into peripheral sensory neurons.
  JOURNAL   Nat Neurosci. 2015 Jan;18(1):25-35. doi: 10.1038/nn.3887. Epub 2014 
            Nov 24.
  PUBMED    25420069
REFERENCE   2  (bases 1 to 9540)
  AUTHORS   .
  TITLE     Direct Submission
  JOURNAL   Exported Dec 15, 2025 from SnapGene Server 8.0.1
            https://www.snapgene.com
COMMENT     SGRef: number: 1; type: "Journal Article"; doi: "10.1038/nn.3887"; 
            journalName: "Nat Neurosci"; date: "2015-01"; volume: "18"; issue: 
            "1"; pages: "25-35"
COMMENT     Sequence Label: Teto BRN3A
FEATURES             Location/Qualifiers
     source          1..9540
                     /mol_type="other DNA"
                     /organism="synthetic DNA construct"
     primer_bind     complement(1..20)
                     /label=pRS-marker
                     /note="pRS vectors, use to sequence yeast selectable 
                     marker"
     enhancer        192..571
                     /label=CMV enhancer
                     /note="human cytomegalovirus immediate early enhancer"
     promoter        573..771
                     /label=CMV promoter
                     /note="human cytomegalovirus (CMV) immediate early 
                     promoter"
     LTR             789..969
                     /label=5' LTR (truncated)
                     /note="truncated 5' long terminal repeat (LTR) from HIV-1"
     misc_feature    1016..1141
                     /label=HIV-1 Psi
                     /note="packaging signal of human immunodeficiency virus 
                     type 1"
     misc_feature    1634..1867
                     /label=RRE
                     /note="The Rev response element (RRE) of HIV-1 allows for 
                     Rev-dependent mRNA export from the nucleus to the 
                     cytoplasm."
     CDS             2052..2096
                     /codon_start=1
                     /product="antigenic peptide corresponding to amino acids 
                     655 to 669 of the HIV envelope protein gp41 (Lutje Hulsik 
                     et al., 2013)"
                     /label=gp41 peptide
                     /note="recognized by the 2H10 single-chain llama nanobody"
                     /translation="KNEQELLELDKWASL"
     misc_feature    2394..2511
                     /label=cPPT/CTS
                     /note="central polypurine tract and central termination 
                     sequence of HIV-1"
     protein_bind    join(2599..2617,2618..2640,2641..2659,2660..2682,2683..2701,
                     2702..2724,2725..2743,2744..2766,2767..2785,2786..2808,
                     2809..2827,2828..2850,2851..2869)
                     /label=tetracycline response element
                     /bound_moiety="tetracycline repressor TetR"
                     /note="contains seven copies of the tetracycline operator 
                     tetO"
     promoter        2902..2940
                     /label=minimal CMV promoter
                     /note="human cytomegalovirus (CMV) immediate early 
                     promoter"
     primer_bind     2937..2961
                     /label=LNCX
                     /note="Human CMV promoter, forward primer"
     CDS             3346..3372
                     /codon_start=1
                     /product="9xHis affinity tag"
                     /label=9xHis
                     /translation="HHHHHHHHH"
     misc_feature    4230..4818
                     /label=WPRE
                     /note="woodchuck hepatitis virus posttranscriptional 
                     regulatory element"
     primer_bind     complement(4283..4303)
                     /label=WPRE-R
                     /note="WPRE, reverse primer"
     CDS             complement(4701..4712)
                     /codon_start=1
                     /product="Factor Xa recognition and cleavage site"
                     /label=Factor Xa site
                     /translation="IEGR"
     primer_bind     complement(4821..4837)
                     /label=KS primer
                     /note="common sequencing primer, one of multiple similar 
                     variants"
     primer_bind     complement(4822..4838)
                     /label=pBluescriptKS
                     /note="For pBluescript vector"
     LTR             5343..5523
                     /label=5' LTR (truncated)
                     /note="truncated 5' long terminal repeat (LTR) from HIV-1"
     primer_bind     complement(5549..5566)
                     /label=BGH-rev
                     /note="Bovine growth hormone terminator, reverse primer. 
                     Also called BGH reverse"
     polyA_signal    5555..5779
                     /label=bGH poly(A) signal
                     /note="bovine growth hormone polyadenylation signal"
     rep_origin      5825..6253
                     /direction=RIGHT
                     /label=f1 ori
                     /note="f1 bacteriophage origin of replication; arrow 
                     indicates direction of (+) strand synthesis"
     primer_bind     complement(5912..5931)
                     /label=F1ori-R
                     /note="F1 origin, reverse primer"
     primer_bind     6122..6143
                     /label=F1ori-F
                     /note="F1 origin, forward primer"
     primer_bind     complement(6262..6282)
                     /label=pBABE 3'
                     /note="SV40 enhancer, reverse primer for pBABE vectors"
     promoter        6267..6596
                     /label=SV40 promoter
                     /note="SV40 enhancer and early promoter"
     rep_origin      6447..6582
                     /label=SV40 ori
                     /note="SV40 origin of replication"
     promoter        6644..6691
                     /label=EM7 promoter
                     /note="synthetic bacterial promoter "
     CDS             6710..7084
                     /codon_start=1
                     /gene="Sh ble from Streptoalloteichus hindustanus"
                     /product="antibiotic-binding protein"
                     /label=BleoR
                     /note="confers resistance to bleomycin, phleomycin, and 
                     Zeocin(TM)"
                     /translation="MAKLTSAVPVLTARDVAGAVEFWTDRLGFSRDFVEDDFAGVVRDD
                     VTLFISAVQDQVVPDNTLAWVWVRGLDELYAEWSEVVSTNFRDASGPAMTEIGEQPWGR
                     EFALRDPAGNCVHFVAEEQD"
     polyA_signal    7214..7335
                     /label=SV40 poly(A) signal
                     /note="SV40 polyadenylation signal"
     primer_bind     complement(7251..7270)
                     /label=SV40pA-R
                     /note="SV40 polyA, reverse primer"
     primer_bind     7305..7324
                     /label=EBV-rev
                     /note="SV40 polyA terminator, reverse primer"
     primer_bind     complement(7384..7400)
                     /label=M13 rev
                     /note="common sequencing primer, one of multiple similar 
                     variants"
     primer_bind     complement(7384..7400)
                     /label=M13 Reverse
                     /note="In lacZ gene. Also called M13-rev"
     primer_bind     complement(7397..7419)
                     /label=M13/pUC Reverse
                     /note="In lacZ gene"
     protein_bind    7408..7424
                     /label=lac operator
                     /bound_moiety="lac repressor encoded by lacI"
                     /note="The lac repressor binds to the lac operator to 
                     inhibit transcription in E. coli. This inhibition can be 
                     relieved by adding lactose or 
                     isopropyl-beta-D-thiogalactopyranoside (IPTG)."
     promoter        complement(join(7432..7438,7439..7456,7457..7462))
                     /label=lac promoter
                     /note="promoter for the E. coli lac operon"
     protein_bind    7477..7498
                     /label=CAP binding site
                     /bound_moiety="E. coli catabolite activator protein"
                     /note="CAP binding activates transcription in the presence 
                     of cAMP."
     primer_bind     complement(7615..7632)
                     /label=L4440
                     /note="L4440 vector, forward primer"
     rep_origin      complement(7786..8374)
                     /direction=LEFT
                     /label=ori
                     /note="high-copy-number ColE1/pMB1/pBR322/pUC origin of 
                     replication"
     primer_bind     complement(7866..7885)
                     /label=pBR322ori-F
                     /note="pBR322 origin, forward primer"
     CDS             complement(join(8545..9336,9337..9405))
                     /codon_start=1
                     /gene="bla"
                     /product="beta-lactamase"
                     /label=AmpR
                     /note="confers resistance to ampicillin, carbenicillin, and
                     related antibiotics"
                     /translation="MSIQHFRVALIPFFAAFCLPVFAHPETLVKVKDAEDQLGARVGYI
                     ELDLNSGKILESFRPEERFPMMSTFKVLLCGAVLSRIDAGQEQLGRRIHYSQNDLVEYS
                     PVTEKHLTDGMTVRELCSAAITMSDNTAANLLLTTIGGPKELTAFLHNMGDHVTRLDRW
                     EPELNEAIPNDERDTTMPVAMATTLRKLLTGELLTLASRQQLIDWMEADKVAGPLLRSA
                     LPAGWFIADKSGAGERGSRGIIAALGPDGKPSRIVVIYTTGSQATMDERNRQIAEIGAS
                     LIKHW"
     primer_bind     9168..9187
                     /label=Amp-R
                     /note="Ampicillin resistance gene, reverse primer"
     promoter        complement(9406..9510)
                     /gene="bla"
                     /label=AmpR promoter
ORIGIN
        1 tacaatctgc tctgatgccg catagttaag ccagtatctg ctccctgctt gtgtgttgga
       61 ggtcgctgag tagtgcgcga gcaaaattta agctacaaca aggcaaggct tgaccgacaa
      121 ttgcatgaag aatctgctta gggttaggcg ttttgcgctg cttcgcgatg tacgggccag
      181 atatacgcgt tgacattgat tattgactag ttattaatag taatcaatta cggggtcatt
      241 agttcatagc ccatatatgg agttccgcgt tacataactt acggtaaatg gcccgcctgg
      301 ctgaccgccc aacgaccccc gcccattgac gtcaataatg acgtatgttc ccatagtaac
      361 gccaataggg actttccatt gacgtcaatg ggtggagtat ttacggtaaa ctgcccactt
      421 ggcagtacat caagtgtatc atatgccaag tacgccccct attgacgtca atgacggtaa
      481 atggcccgcc tggcattatg cccagtacat gaccttatgg gactttccta cttggcagta
      541 catctacgta ttagtcatcg ctattaccat ggtgatgcgg ttttggcagt acatcaatgg
      601 gcgtggatag cggtttgact cacggggatt tccaagtctc caccccattg acgtcaatgg
      661 gagtttgttt tggcaccaaa atcaacggga ctttccaaaa tgtcgtaaca actccgcccc
      721 attgacgcaa atgggcggta ggcgtgtacg gtgggaggtc tatataagca gcgcgttttg
      781 cctgtactgg gtctctctgg ttagaccaga tctgagcctg ggagctctct ggctaactag
      841 ggaacccact gcttaagcct caataaagct tgccttgagt gcttcaagta gtgtgtgccc
      901 gtctgttgtg tgactctggt aactagagat ccctcagacc cttttagtca gtgtggaaaa
      961 tctctagcag tggcgcccga acagggactt gaaagcgaaa gggaaaccag aggagctctc
     1021 tcgacgcagg actcggcttg ctgaagcgcg cacggcaaga ggcgaggggc ggcgactggt
     1081 gagtacgcca aaaattttga ctagcggagg ctagaaggag agagatgggt gcgagagcgt
     1141 cagtattaag cgggggagaa ttagatcgcg atgggaaaaa attcggttaa ggccaggggg
     1201 aaagaaaaaa tataaattaa aacatatagt atgggcaagc agggagctag aacgattcgc
     1261 agttaatcct ggcctgttag aaacatcaga aggctgtaga caaatactgg gacagctaca
     1321 accatccctt cagacaggat cagaagaact tagatcatta tataatacag tagcaaccct
     1381 ctattgtgtg catcaaagga tagagataaa agacaccaag gaagctttag acaagataga
     1441 ggaagagcaa aacaaaagta agaccaccgc acagcaagcg gccgctgatc ttcagacctg
     1501 gaggaggaga tatgagggac aattggagaa gtgaattata taaatataaa gtagtaaaaa
     1561 ttgaaccatt aggagtagca cccaccaagg caaagagaag agtggtgcag agagaaaaaa
     1621 gagcagtggg aataggagct ttgttccttg ggttcttggg agcagcagga agcactatgg
     1681 gcgcagcgtc aatgacgctg acggtacagg ccagacaatt attgtctggt atagtgcagc
     1741 agcagaacaa tttgctgagg gctattgagg cgcaacagca tctgttgcaa ctcacagtct
     1801 ggggcatcaa gcagctccag gcaagaatcc tggctgtgga aagataccta aaggatcaac
     1861 agctcctggg gatttggggt tgctctggaa aactcatttg caccactgct gtgccttgga
     1921 atgctagttg gagtaataaa tctctggaac agatttggaa tcacacgacc tggatggagt
     1981 gggacagaga aattaacaat tacacaagct taatacactc cttaattgaa gaatcgcaaa
     2041 accagcaaga aaagaatgaa caagaattat tggaattaga taaatgggca agtttgtgga
     2101 attggtttaa cataacaaat tggctgtggt atataaaatt attcataatg atagtaggag
     2161 gcttggtagg tttaagaata gtttttgctg tactttctat agtgaataga gttaggcagg
     2221 gatattcacc attatcgttt cagacccacc tcccaacccc gaggggaccc gacaggcccg
     2281 aaggaataga agaagaaggt ggagagagag acagagacag atccattcga ttagtgaacg
     2341 gatcggcact gcgtgcgcca attctgcaga caaatggcag tattcatcca caattttaaa
     2401 agaaaagggg ggattggggg gtacagtgca ggggaaagaa tagtagacat aatagcaaca
     2461 gacatacaaa ctaaagaatt acaaaaacaa attacaaaaa ttcaaaattt tcgggtttat
     2521 tacagggaca gcagagatcc agtttggtta attaaaggcg tatcacgagg ccctttcgtc
     2581 ttcactcgag tttaccactc cctatcagtg atagagaaaa gtgaaagtcg agtttaccac
     2641 tccctatcag tgatagagaa aagtgaaagt cgagtttacc actccctatc agtgatagag
     2701 aaaagtgaaa gtcgagttta ccactcccta tcagtgatag agaaaagtga aagtcgagtt
     2761 taccactccc tatcagtgat agagaaaagt gaaagtcgag tttaccactc cctatcagtg
     2821 atagagaaaa gtgaaagtcg agtttaccac tccctatcag tgatagagaa aagtgaaagt
     2881 cgagctcggt acccgggtcg aggtaggcgt gtacggtggg aggcctatat aagcagagct
     2941 cgtttagtga accgtcagat cgcctggaga cgccatccac gctgttttga cctccataga
     3001 agacaccggg accgatccag cctcctgcag gtctagagga tcgaattcat gatgtccatg
     3061 aacagcaagc agcctcactt tgccatgcat cccaccctcc ctgagcacaa gtacccgtcg
     3121 ctgcactcca gctccgaggc catccggcgg gcctgcctgc ccacgccgcc gctgcagagc
     3181 aacctcttcg ccagcctgga cgagacgctg ctggcgcggg ccgaggcgct ggcggccgtg
     3241 gacatcgccg tgtcccaggg caagagccat cctttcaagc cggacgccac gtaccacacg
     3301 atgaacagcg tgccgtgcac gtccacttcc acggtgcctc tggcgcacca ccaccaccac
     3361 caccaccacc accaggcgct cgaacccggc gatctgctgg accacatctc ctcgccgtcg
     3421 ctcgcgctca tggccggcgc gggcggcgcg ggcgcggcgg ccggcggcgg gctcctgggc
     3481 ggctccgcgc accctcaccc gcatatgcac agcctgggcc acctgtcgca ccccgcggcg
     3541 gcggccgcca tgaacatgcc gtccgggctg ccgcaccccg ggctggtggc ggcggcggcg
     3601 caccacggcg cggcagcggc agcggcggcg gcggcggccg ggcaggtggc agcggcatcg
     3661 gcggcggcgg ccgtggtggg cgcagcgggc ctggcgtcca tctgcgactc ggacacggac
     3721 ccgcgcgagc tcgaggcgtt cgcggagcgc ttcaagcagc ggcgcatcaa gctgggcgtg
     3781 acgcaggccg acgtgggctc ggcgctggcc aacctcaaga tcccgggcgt gggctcactc
     3841 agccagagca ccatctgcag gttcgagtcg ctcacgctct cgcacaacaa catgatcgcg
     3901 ctcaagccca tcctgcaggc gtggctcgag gaggccgagg gcgcccagcg cgagaaaatg
     3961 aacaagcctg agctcttcaa cggcggcgag aagaagcgca agcggacttc catcgccgcg
     4021 cccgagaagc gctccctcga ggcctacttc gccgtgcagc cccggccctc gtccgagaag
     4081 atcgccgcca tcgccgagaa actggacctc aaaaagaacg tggtgcgggt gtggttttgc
     4141 aaccagagac agaagcagaa gcggatgaaa ttctctgcca cttactgagc tagcggatcc
     4201 gttaacaatt cgatatcaag cttatcgata atcaacctct ggattacaaa atttgtgaaa
     4261 gattgactgg tattcttaac tatgttgctc cttttacgct atgtggatac gctgctttaa
     4321 tgcctttgta tcatgctatt gcttcccgta tggctttcat tttctcctcc ttgtataaat
     4381 cctggttgct gtctctttat gaggagttgt ggcccgttgt caggcaacgt ggcgtggtgt
     4441 gcactgtgtt tgctgacgca acccccactg gttggggcat tgccaccacc tgtcagctcc
     4501 tttccgggac tttcgctttc cccctcccta ttgccacggc ggaactcatc gccgcctgcc
     4561 ttgcccgctg ctggacaggg gctcggctgt tgggcactga caattccgtg gtgttgtcgg
     4621 ggaaatcatc gtcctttcct tggctgctcg cctgtgttgc cacctggatt ctgcgcggga
     4681 cgtccttctg ctacgtccct tcggccctca atccagcgga ccttccttcc cgcggcctgc
     4741 tgccggctct gcggcctctt ccgcgtcttc gccttcgccc tcagacgagt cggatctccc
     4801 tttgggccgc ctccccgcat cgataccgtc gacctcgaga cctagaaaaa catggagcaa
     4861 tcacaagtag caatacagca gctaccaatg ctgattgtgc ctggctagaa gcacaagagg
     4921 aggaggaggt gggttttcca gtcacacctc aggtaccttt aagaccaatg acttacaagg
     4981 cagctgtaga tcttagccac tttttaaaag aaaagggggg actggaaggg ctaattcact
     5041 cccaacgaag acaagatatc cttgatctgt ggatctacca cacacaaggc tacttccctg
     5101 attggcagaa ctacacacca gggccaggga tcagatatcc actgaccttt ggatggtgct
     5161 acaagctagt accagttgag caagagaagg tagaagaagc caatgaagga gagaacaccc
     5221 gcttgttaca ccctgtgagc ctgcatggga tggatgaccc ggagagagaa gtattagagt
     5281 ggaggtttga cagccgccta gcatttcatc acatggcccg agagctgcat ccggactgta
     5341 ctgggtctct ctggttagac cagatctgag cctgggagct ctctggctaa ctagggaacc
     5401 cactgcttaa gcctcaataa agcttgcctt gagtgcttca agtagtgtgt gcccgtctgt
     5461 tgtgtgactc tggtaactag agatccctca gaccctttta gtcagtgtgg aaaatctcta
     5521 gcagggcccg tttaaacccg ctgatcagcc tcgactgtgc cttctagttg ccagccatct
     5581 gttgtttgcc cctcccccgt gccttccttg accctggaag gtgccactcc cactgtcctt
     5641 tcctaataaa atgaggaaat tgcatcgcat tgtctgagta ggtgtcattc tattctgggg
     5701 ggtggggtgg ggcaggacag caagggggag gattgggaag acaatagcag gcatgctggg
     5761 gatgcggtgg gctctatggc ttctgaggcg gaaagaacca gctggggctc tagggggtat
     5821 ccccacgcgc cctgtagcgg cgcattaagc gcggcgggtg tggtggttac gcgcagcgtg
     5881 accgctacac ttgccagcgc cctagcgccc gctcctttcg ctttcttccc ttcctttctc
     5941 gccacgttcg ccggctttcc ccgtcaagct ctaaatcggg ggctcccttt agggttccga
     6001 tttagtgctt tacggcacct cgaccccaaa aaacttgatt agggtgatgg ttcacgtagt
     6061 gggccatcgc cctgatagac ggtttttcgc cctttgacgt tggagtccac gttctttaat
     6121 agtggactct tgttccaaac tggaacaaca ctcaacccta tctcggtcta ttcttttgat
     6181 ttataaggga ttttgccgat ttcggcctat tggttaaaaa atgagctgat ttaacaaaaa
     6241 tttaacgcga attaattctg tggaatgtgt gtcagttagg gtgtggaaag tccccaggct
     6301 ccccagcagg cagaagtatg caaagcatgc atctcaatta gtcagcaacc aggtgtggaa
     6361 agtccccagg ctccccagca ggcagaagta tgcaaagcat gcatctcaat tagtcagcaa
     6421 ccatagtccc gcccctaact ccgcccatcc cgcccctaac tccgcccagt tccgcccatt
     6481 ctccgcccca tggctgacta atttttttta tttatgcaga ggccgaggcc gcctctgcct
     6541 ctgagctatt ccagaagtag tgaggaggct tttttggagg cctaggcttt tgcaaaaagc
     6601 tcccgggagc ttgtatatcc attttcggat ctgatcagca cgtgttgaca attaatcatc
     6661 ggcatagtat atcggcatag tataatacga caaggtgagg aactaaacca tggccaagtt
     6721 gaccagtgcc gttccggtgc tcaccgcgcg cgacgtcgcc ggagcggtcg agttctggac
     6781 cgaccggctc gggttctccc gggacttcgt ggaggacgac ttcgccggtg tggtccggga
     6841 cgacgtgacc ctgttcatca gcgcggtcca ggaccaggtg gtgccggaca acaccctggc
     6901 ctgggtgtgg gtgcgcggcc tggacgagct gtacgccgag tggtcggagg tcgtgtccac
     6961 gaacttccgg gacgcctccg ggccggccat gaccgagatc ggcgagcagc cgtgggggcg
     7021 ggagttcgcc ctgcgcgacc cggccggcaa ctgcgtgcac ttcgtggccg aggagcagga
     7081 ctgacacgtg ctacgagatt tcgattccac cgccgccttc tatgaaaggt tgggcttcgg
     7141 aatcgttttc cgggacgccg gctggatgat cctccagcgc ggggatctca tgctggagtt
     7201 cttcgcccac cccaacttgt ttattgcagc ttataatggt tacaaataaa gcaatagcat
     7261 cacaaatttc acaaataaag catttttttc actgcattct agttgtggtt tgtccaaact
     7321 catcaatgta tcttatcatg tctgtatacc gtcgacctct agctagagct tggcgtaatc
     7381 atggtcatag ctgtttcctg tgtgaaattg ttatccgctc acaattccac acaacatacg
     7441 agccggaagc ataaagtgta aagcctgggg tgcctaatga gtgagctaac tcacattaat
     7501 tgcgttgcgc tcactgcccg ctttccagtc gggaaacctg tcgtgccagc tgcattaatg
     7561 aatcggccaa cgcgcgggga gaggcggttt gcgtattggg cgctcttccg cttcctcgct
     7621 cactgactcg ctgcgctcgg tcgttcggct gcggcgagcg gtatcagctc actcaaaggc
     7681 ggtaatacgg ttatccacag aatcagggga taacgcagga aagaacatgt gagcaaaagg
     7741 ccagcaaaag gccaggaacc gtaaaaaggc cgcgttgctg gcgtttttcc ataggctccg
     7801 cccccctgac gagcatcaca aaaatcgacg ctcaagtcag aggtggcgaa acccgacagg
     7861 actataaaga taccaggcgt ttccccctgg aagctccctc gtgcgctctc ctgttccgac
     7921 cctgccgctt accggatacc tgtccgcctt tctcccttcg ggaagcgtgg cgctttctca
     7981 tagctcacgc tgtaggtatc tcagttcggt gtaggtcgtt cgctccaagc tgggctgtgt
     8041 gcacgaaccc cccgttcagc ccgaccgctg cgccttatcc ggtaactatc gtcttgagtc
     8101 caacccggta agacacgact tatcgccact ggcagcagcc actggtaaca ggattagcag
     8161 agcgaggtat gtaggcggtg ctacagagtt cttgaagtgg tggcctaact acggctacac
     8221 tagaagaaca gtatttggta tctgcgctct gctgaagcca gttaccttcg gaaaaagagt
     8281 tggtagctct tgatccggca aacaaaccac cgctggtagc ggtggttttt ttgtttgcaa
     8341 gcagcagatt acgcgcagaa aaaaaggatc tcaagaagat cctttgatct tttctacggg
     8401 gtctgacgct cagtggaacg aaaactcacg ttaagggatt ttggtcatga gattatcaaa
     8461 aaggatcttc acctagatcc ttttaaatta aaaatgaagt tttaaatcaa tctaaagtat
     8521 atatgagtaa acttggtctg acagttacca atgcttaatc agtgaggcac ctatctcagc
     8581 gatctgtcta tttcgttcat ccatagttgc ctgactcccc gtcgtgtaga taactacgat
     8641 acgggagggc ttaccatctg gccccagtgc tgcaatgata ccgcgagacc cacgctcacc
     8701 ggctccagat ttatcagcaa taaaccagcc agccggaagg gccgagcgca gaagtggtcc
     8761 tgcaacttta tccgcctcca tccagtctat taattgttgc cgggaagcta gagtaagtag
     8821 ttcgccagtt aatagtttgc gcaacgttgt tgccattgct acaggcatcg tggtgtcacg
     8881 ctcgtcgttt ggtatggctt cattcagctc cggttcccaa cgatcaaggc gagttacatg
     8941 atcccccatg ttgtgcaaaa aagcggttag ctccttcggt cctccgatcg ttgtcagaag
     9001 taagttggcc gcagtgttat cactcatggt tatggcagca ctgcataatt ctcttactgt
     9061 catgccatcc gtaagatgct tttctgtgac tggtgagtac tcaaccaagt cattctgaga
     9121 atagtgtatg cggcgaccga gttgctcttg cccggcgtca atacgggata ataccgcgcc
     9181 acatagcaga actttaaaag tgctcatcat tggaaaacgt tcttcggggc gaaaactctc
     9241 aaggatctta ccgctgttga gatccagttc gatgtaaccc actcgtgcac ccaactgatc
     9301 ttcagcatct tttactttca ccagcgtttc tgggtgagca aaaacaggaa ggcaaaatgc
     9361 cgcaaaaaag ggaataaggg cgacacggaa atgttgaata ctcatactct tcctttttca
     9421 atattattga agcatttatc agggttattg tctcatgagc ggatacatat ttgaatgtat
     9481 ttagaaaaat aaacaaatag gggttccgcg cacatttccc cgaaaagtgc cacctgacac
//
