LOCUS       sequence-102003-         946 bp    DNA     linear   UNA 15-DEC-2025
DEFINITION  Localization: Focal Adhesions, Excitation: 448 / 473, Emission: 500 
            / 528.
ACCESSION   .
VERSION     .
KEYWORDS    .
SOURCE      natural DNA sequence
  ORGANISM  unspecified
REFERENCE   1  (bases 1 to 946)
  AUTHORS   Shu X, Lev-Ram V, Deerinck TJ, Qi Y, Ramko EB, Davidson MW, Jin Y, 
            Ellisman MH, Tsien RY
  TITLE     A genetically encoded tag for correlated light and electron 
            microscopy of intact cells, tissues, and organisms.
  JOURNAL   PLoS Biol. 2011 Apr;9(4):e1001041. doi: 
            10.1371/journal.pbio.1001041. Epub 2011 Apr 5.
  PUBMED    21483721
REFERENCE   2  (bases 1 to 946)
  AUTHORS   .
  TITLE     Direct Submission
  JOURNAL   Exported Dec 15, 2025 from SnapGene Server 8.0.1
            https://www.snapgene.com
COMMENT     SGRef: number: 1; type: "Journal Article"; journalName: "PLoS 
            Biol."; date: "2011-04"; volume: "9(4):e1001041. doi"; pages: " 
            10.1371/journal.pbio.1001041. Epub 2011 Apr 5"
COMMENT     Sequence Label: mCherry-miniSOG-Vinculin-23
FEATURES             Location/Qualifiers
     source          1..946
                     /mol_type="genomic DNA"
                     /organism="unspecified"
     regulatory      33..42
                     /label=Kozak sequence
                     /note="vertebrate consensus sequence for strong initiation 
                     of translation (Kozak, 1987)"
                     /regulatory_class="other"
     CDS             39..356
                     /codon_start=1
                     /product="mini singlet oxygen generator, a fluorescent 
                     flavoprotein suitable for correlated light and electron 
                     microscopy (Shu et al., 2011)"
                     /label=miniSOG
                     /translation="MEKSFVITDPRLPDNPIIFASDGFLELTEYSREEILGRNGRFLQG
                     PETDQATVQKIRDAIRDQREITVQLINYTKSGKKFWNLLHLQPMRDQKGELQYFIGVQL
                     DG"
     primer_bind     complement(508..526)
                     /label=mCherry-R
                     /note="mCherry, reverse primer"
     primer_bind     776..795
                     /label=mCherry-F
                     /note="mCherry, forward primer"
ORIGIN
        1 gtgaaccgtc agatccgcta gcgctaccgg tcgccaccat ggagaagagc ttcgtgatca
       61 ccgacccccg cctgcccgac aaccccatca tcttcgccag cgacggcttc ctggagctga
      121 ccgagtacag cagagaggag atcctgggcc gcaacggccg cttcctgcag ggccccgaga
      181 ccgaccaggc caccgtgcag aagatccgcg acgccatccg cgaccagcgc gagatcaccg
      241 tgcagctgat caactacacc aagagcggca agaagttctg gaacctgctg cacctgcagc
      301 ccatgcgcga ccagaagggc gagctgcagt acttcatcgg cgtgcagctg gacggcggcg
      361 gcatggtgag caagggcgag gaggataaca tggccatcat caaggagttc atgcgcttca
      421 aggtgcacat ggagggctcc gtgaacggcc acgagttcga gatcgagggc gagggcgagg
      481 gccgccccta cgagggcacc cagaccgcca agctgaaggt gaccaagggt ggccccctgc
      541 ccttcgcctg ggacatcctg tcccctcagt tcatgtacgg ctccaaggcc tacgtgaagc
      601 accccgccga catccccgac tacttgaagc tgtccttccc cgagggcttc aagtgggagc
      661 gcgtgatgaa cttcgaggac ggcggcgtgg tgaccgtgac ccaggactcc tccctgcagg
      721 acggcgagtt catctacaag gtgaagctgc gcggcaccaa cttcccctcc gacggccccg
      781 taatgcagaa gaagaccatg ggctgggagg cctcctccga gcggatgtac cccgaggacg
      841 gcgccctgaa gggcgagatc aagcagaagc tgaagctgaa ggacggcggc cactacgacg
      901 ctgaggtcaa gaccacctac aaggccaaga agcccgtgca gctgcc
//
