LOCUS       sequence-102008-         820 bp    DNA     linear   UNA 15-DEC-2025
DEFINITION  5' Gateway Entry Clone Containing 4xnrUAS.
ACCESSION   .
VERSION     .
KEYWORDS    .
SOURCE      natural DNA sequence
  ORGANISM  unspecified
REFERENCE   1  (bases 1 to 820)
  AUTHORS   Akitake CM, Macurak M, Halpern ME, Goll MG
  TITLE     Transgenerational analysis of transcriptional silencing in 
            zebrafish.
  JOURNAL   Dev Biol. 2011 Apr 15;352(2):191-201. doi: 
            10.1016/j.ydbio.2011.01.002. Epub 2011 Jan 9.
  PUBMED    21223961
REFERENCE   2  (bases 1 to 820)
  AUTHORS   .
  TITLE     Direct Submission
  JOURNAL   Exported Dec 15, 2025 from SnapGene Server 8.0.1
            https://www.snapgene.com
COMMENT     SGRef: number: 1; type: "Journal Article"; doi: 
            "10.1016/j.ydbio.2011.01.002"; journalName: "Dev Biol"; date: 
            "2011-04-15- 15"; volume: "352"; issue: "2"; pages: "191-201"
COMMENT     Sequence Label: p5E-4xnrUAS (L4-R1)
FEATURES             Location/Qualifiers
     source          1..820
                     /mol_type="genomic DNA"
                     /organism="unspecified"
     primer_bind     61..83
                     /label=M13/pUC Forward
                     /note="In lacZ gene"
     primer_bind     75..92
                     /label=M13 Forward
                     /note="In lacZ gene. Also called M13-F20 or M13 (-21) 
                     Forward"
     primer_bind     76..92
                     /label=M13 fwd
                     /note="common sequencing primer, one of multiple similar 
                     variants"
     protein_bind    132..227
                     /gene="mutant version of attL"
                     /label=attL4
                     /bound_moiety="LR Clonase(TM)"
                     /note="recombination site for the Gateway(R) LR reaction"
     protein_bind    404..527
                     /gene="mutant version of attR"
                     /label=attR1
                     /bound_moiety="LR Clonase(TM)"
                     /note="recombination site for the Gateway(R) LR reaction"
     primer_bind     complement(599..618)
                     /label=T7
                     /note="T7 promoter, forward primer"
     promoter        complement(600..618)
                     /label=T7 promoter
                     /note="promoter for bacteriophage T7 RNA polymerase"
     primer_bind     complement(623..639)
                     /label=M13 rev
                     /note="common sequencing primer, one of multiple similar 
                     variants"
     primer_bind     complement(623..639)
                     /label=M13 Reverse
                     /note="In lacZ gene. Also called M13-rev"
     primer_bind     complement(741..760)
                     /label=pENTR-R
                     /note="pENTR vectors, reverse primer"
ORIGIN
        1 tcgttttatt tgatgcctgg cagttcccta ctctcgcgtt aacgctagca tggatgtttt
       61 cccagtcacg acgttgtaaa acgacggcca gtcttaagct cgggcccgag ttaacgctac
      121 catggagctc caaataatga ttttattttg actgatagtg acctgttcgt tgcaacaaat
      181 tgataagcaa tgctttttta taatgccaac tttgtataga aaagttgacg gtggcttcta
      241 atccgtgagt cctagcgggt gacagccctc cgtcttcaca ggcggaggag agtcttccgt
      301 agggttcctc ggagtactgt cctccgacgc gtgcaagggt cgactctaga gggtatataa
      361 tggatcccat cgcgtctcag cctcactttg agctcctcca caccaagttt gtacaaaaaa
      421 gttgaacgag aaacgtaaaa tgatataaat atcaatatat taaattagat tttgcataaa
      481 aaacagacta cataatactg taaaacacaa catatgcagt cactatgaac caactactta
      541 gatggtatta gtgacctgta gaattcgagc tctagagctg cagggcggcc gcgatatccc
      601 ctatagtgag tcgtattaca tggtcatagc tgtttcctgg cagctctggc ccgtgtctca
      661 aaatctctga tgttacattg cacaagataa aaatatatca tcatgaacaa taaaactgtc
      721 tgcttacata aacagtaata caaggggtgt tatgagccat attcaacggg aaacgtcgag
      781 gccgcgatta aattccaaca tggatgctga tttatatggg
//
